Antioxidant Outcomes of L-arginine on Monosodium Glutamate-induced Oxidative Stress in Rats’ Testes
Keywords:
Superoxide dismutase, malondialdehyde, reduced glutathione, catalaseAbstract
Oxidative stress was implicated in monosodium glutamate-related adversity in male reproductive organs. This study examined the possible antioxidant outcomes of L-arginine on oxidative stress induced by monosodium glutamate burden (8000 mg/kg of body weight) in rats’ testes after oral intubation for 28 days. Exposure to monosodium glutamate significantly (p < 0.05) increased malondialdehyde (3.84±0.34 mg/dl) level but diminished the level of reduced glutathione (9.60 ± 0.28 mg/dL), catalase (5.51 ± 0.35 IU/L) and superoxide dismutase (1.74 ± 0.20 IU/L) in the rats’ testes homogenate compared to control and other groups. L-arginine treatment alone or l-arginine co-treatments with monosodium glutamate significantly (p < 0.05) decreased the level of malondialdehyde but increased that of reduced glutathione, catalase and superoxide dismutase in the rats’ testes compared to control and monosodium glutamate treated groups. Thus, the oxidant effects induced by monosodium glutamate at a dose of 8000 mg/kg in the rats’ testes were significantly mitigated by L-arginine. This portends the antioxidative role of L-arginine in oxidative stress related testes dysfunctions by probable deregulation in malondialdehyde but up-regulation in reduced glutathione, catalase, and superoxide dismutase expression and functions.
Downloads
References
Abdul-Hamid, M., Galaly, S. R., Ahmed, R. R. and Hamdalla, H. M. (2021). Histopathological and biochemical effect of quercetin on monosodium glutamate supplementation‑induced testicular toxicity. Beni-Suef University Journal of Basic and Applied Sciences, 10(73), 1-15.
Chen, J. Q. and Li, C. M. (2018). Dietary l-arginine supplementation improves semen quality and libido of boars under high ambient temperature. Animal, 12(8), 1611-1620.
Demir, F., Uzun, F. G., Durak, D. and Kalender, Y. (2011). Subacute chlorpyrifos induced oxidative stress in rat erythrocytes and the protective effects of catechin and quercetin. Pesticide Biochemistry and Physics, 99(1), 77-81.
Egbuonu, A. C. C. and Ejike, G. E. (2017). Effect of pulverized Mangifera indica (mango) seed kernel on monosodium glutamate-intoxicated rats’ serum antioxidant capacity, brain function and histology. EC Pharmacology and Toxicology, 4(6), 228-243.
Egbuonu, A. C. C., Obidoa, O., Ezeokonkwo, C. A., Ejikeme, P. M. and Ezeanyika, L. U. S. (2010). Some biochemical effects of sub-acute oral administration of L-arginine on monosodium glutamate-fed Wistar albino rats 1: Body weight change, serum cholesterol, creatinine and sodium ion concentrations. Toxicological and Environmental Chemistry, 92(7), 1331-1337.
Egbuonu, A. C. C., Opara, C. I., Akachukwu, D. and Onyedikachi, U. B. (2018). Effect of ethanolic extract of avocado pear (Persea americana) seed on normal and monosodium glutamate-compromised rats’ hepatic histo-morphology and serum bio-functional parameters. Research Journal of Environmental Sciences, 2(2), 53-62.
Ellman, G. L. (1959). Tissue sulfhydryl groups. Archive of Biochemistry and Biophysics, 82, 7-77
Ezeanyika, L. U. S. and Egbuonu, A. C. C. (2011). Impact of nitric oxide and insulin resistance on the pathophysiology of the metabolic syndrome: Possible role of l-arginine and glutamate. Journal of Medicine and Medical Sciences, 2(2), 657-662.
Fabio, R., Seiva, F., Gustavo, L. Chuffa, A., Braga, C. P., Paulo, J., Amorim, A. and Fernandes, A. A. H. (2012). Quercetin ameliorates glucose and lipid metabolism and improves antioxidant status in postnatal monosodium glutamate-induced metabolic alterations. Food and Chemical Toxicology, 50(10), 3556-3561.
Ighodaro, O. M. and Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287-293.
Ilaria, M., Fabio, A. and Ilaria, P. (2017). Measurement and clinical significance of biomarkers of oxidative stress in humans. Oxidative Medicine and Cellular Longevity, Article ID 6501046: 1-32.
Johansson, L. H. and Borg, L.A. (1988). A spectrophotometric method for determination of catalase activity in small sample tissues. Analytical Biochemistry, 174(1), 331-336.
Joseph, N., Zhang-James, Y., Pearl, A. and Faraone, S. V. (2015). Oxidative stress and ADHD: A meta-analysis. Journal of Attention Disorder, 19(11), 915-924.
Jubaidi, J. F., Mathialagan, R. D., Noor, M. M., Taib, I. and Budin, S. B. (2019). Monosodium glutamate daily oral supplementation: Study of its effects on male reproductive system on rat model. Systems Biology in Reproductive Medicine, 65(3), 194-204.
Liang, M., Wang, Z., Li, H., Cai, L., Pan, J., He, H., Wu, Q., Tang, Y., Ma, J. and Yang, L. (2018). L-arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway. Food and Chemical Toxicology, 115, 315-328.
Madesh, M. and Balasubramanian, K. A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics, 35(3), 184-188.
Malik, S. S., Nawaz, G. and Masood, N. (2016). Genotypes of GSTMI and GSTTI: Useful Determinants for Clinical Outcome for Bladder Cancer in Pakistani Population. Egyptian Journal of Medical and Human Genetics, 10(1), 1016.
Mandal, A., Das, S. Roy, S., Ghosh, A. K., Sardar, A. H., Verma, S., Saini, S., Singh, R., Abhishek, K., Kumar, A., Mandal, C. and Das, P. (2016). Deprivation of l-arginine induces oxidative stress mediated apoptosis in Leishmania donovani promastigotes: Contribution of the polyamine pathway. PLoS Neglected Tropical Diseases, 10, 1-26.
National Research Council (NRC), USA). (2011). Guide for the care and use of laboratory animals. 8th Edition, Committee for the Update of the Guide for the Care and Use of Laboratory Animals, National Research Council, National Academies Press, Washington DC, USA.
Otasevic, V., Korac, A., Vucetic, M., Macanovic, B., Garalyic, E. and Ivanovic-Burmazovic, I. (2013). Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility. Antioxidant and Redox Signalling, 18(2), 170-178.
Phaniendra, A., Jestadi, D. B. and Peryasamy, L. (2015). Free radicals: properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry, 30, 11-26.
Poljak, B. and Fink, R. (2014). The protective role of antioxidants in the defence against ROS/RNS-mediated environmental pollution. Oxidative Medicine and Cellular Longevity, Article ID 671539, 1-22.
Qiu, Y., Yang, X., Wang, L., Gao, K. and Jiang, Z. (2019). L-arginine inhibited inflammatory response and oxidative stress induced by lipopolysaccharide via arginase-1 signaling in IPEC-J2 cells. International Journal of Molecular Sciences, 20(7), 1800-1813.
Sedlak, J. and Lindsay, R. H. (1968). Estimation of total protein-bound, and non protein sulfhydryl groups in tissue with Ellman’s Reagent. Analytical Biochemistry, 25, 1192-1205.
Wallin, B., Rosengren, B., Shertzer, H. G. and Camejo, G. (1993). Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtiter plate: its use for evaluation of antioxidants. Analytical Biochemistry, 208(1), 10-15.
Zhang, H., Peng, A., Yu, Y., Guo, S., Wang, M. Z. and Wang, H. R. (2019). L-arginine protects ovine intestinal epithelial cells from lipopolysaccharides-induced apoptosis through alleviating oxidative stress. Journal of Agricultural and Food Chemistry, 67(6), 1683-1690.
Additional Files
Published
Issue
Section
Categories
License
Copyright (c) 2022 Anthony Cemaluk C. Egbuonu
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
