• Human Salivary Thiocyanate Level of Adult Volunteers in Sokoto, Nigeria
    Vol. 19 No. 1 (2004)


    Salivary thiocyanate levels of 379 volunteers aged 18-55 years were estimated spectrophotometrically. The results obtained were analyzed on the basis of sex, smoking, kola nut eating and gari eating habits. The levels of salivary thiocyanate levels of smokers, smokers who eat gari, smoker who eat kola nut and smokers who eat gari and kola nut are 3.61±2.07mM, 3.27±1.96mM, 4.19±2.15mM and 4.18±2.39mM respectively. These values are significantly higher than those of non-smokers who, eats kola nut (1.35±1.05mM), eat gari (1.49±1.09mM), eat gari and kola nut (1.64±1.02mM) and neither eat gari nor kola
    nut (1.30±O.93mM). The values obtained were significantly higher for females. Significant percentage of smokers (70-80%) had salivary thiocyanate level >2.0mmolll. Only about 20% of non-smokers had salivary thiocyanate levels >2.0mmolll. Analysis of the results did not show any synergistic effect of these habits on salivary thiocyanate of the subjects combining these habits. No correlation was also found between salivary thiocyanate level and age. Cyanide exposure may therefore be another risk factor in
    tobacco smoking.

  • Biochemical Mechanisms of Wound Healing Using Extracts ofChromolaena odorata-Linn
    Vol. 24 No. 2 (2009)

    Protection against hydroxyl radical-induced oxidative damage, reduction in glucose concentration and inhibition of dehydrogenase enzymes as possible mechanisms of wound healing using ethanol extracts of Chromolaena odorata-Linn were studied. Hydroxyl radical (OH˙)-scavenging activity was measured by studying the competi[1]tion between deoxyribose degradation and C. odorata for hydroxyl radicals generated via the Fe3+ / ascorbate /EDTA / H2O2 system. Hypoglycaemic potential was assessed by the glucose tolerance test in white New Zealand rabbits. Inhibition of dehydrogenase activity (DHA) in menacing wound pathogenic bacteria was investigated via dehydrogenase assay using 2,3,5-triphenyl tetrazolium chloride (TTC) as the artificial electron acceptor. Pure cultures of the organisms (Escherichia sp., Staphylococcus sp., and Pseudomonas sp.) were exposed to graded concentrations of ethanol extracts of C. odorata (ETECO 0-2000µg/ml). Our results reveal the potential of the extract to protect against hydroxyl radical-induced damage as demonstrated by its ability to scavenge hydroxyl radical in-vitro. The ethanol extract of C. odorata was shown to be hypoglycaemic in the glucose tolerance test. C. odorata exhibited a concentration-dependent response against dehydrogenase activity in the tested organisms. Dehydrogenase activity (mg Formazan/mg cell dry weight /h) was inhibited in a manner that obeyed a logistic dose response model (abcd). Inhibitory concentrations (IC20, IC50, IC70, and IC100) of ETECO showed that the plant C.odorata is a good inhibitor of dehydrogenase activity in the tested pathogens. %Inhibition of hydroxyl radicals: followed a logistic dose response model (abcd) similar to percentage inhibition of DHA.