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Vol. 24 No. 2 (2009): Biochemical Mechanisms of Wound Healing Using Extracts ofChromolaena odorata-Linn
Protection against hydroxyl radical-induced oxidative damage, reduction in glucose concentration and inhibition of dehydrogenase enzymes as possible mechanisms of wound healing using ethanol extracts of Chromolaena odorata-Linn were studied. Hydroxyl radical (OH˙)-scavenging activity was measured by studying the competition between deoxyribose degradation and C. odorata for hydroxyl radicals generated via the Fe3+ / ascorbate /EDTA / H2O2 system. Hypoglycaemic potential was assessed by the glucose tolerance test in white New Zealand rabbits. Inhibition of dehydrogenase activity (DHA) in menacing wound pathogenic bacteria was investigated via dehydrogenase assay using 2,3,5-triphenyl tetrazolium chloride (TTC) as the artificial electron acceptor. Pure cultures of the organisms (Escherichia sp., Staphylococcus sp., and Pseudomonas sp.) were exposed to graded concentrations of ethanol extracts of C. odorata (ETECO 0-2000µg/ml). Our results reveal the potential of the extract to protect against hydroxyl radical-induced damage as demonstrated by its ability to scavenge hydroxyl radical in-vitro. The ethanol extract of C. odorata was shown to be hypoglycaemic in the glucose tolerance test. C. odorata exhibited a concentration-dependent response against dehydrogenase activity in the tested organisms. Dehydrogenase activity (mg Formazan/mg cell dry weight /h) was inhibited in a manner that obeyed a logistic dose response model (abcd). Inhibitory concentrations (IC20, IC50, IC70, and IC100) of ETECO showed that the plant C.odorata is a good inhibitor of dehydrogenase activity in the tested pathogens. %Inhibition of hydroxyl radicals: followed a logistic dose response model (abcd) similar to percentage inhibition of DHA.