Antioxidant, Anti-inflammatory, and Anticancer Potentials of Ethyl acetate Extract of Andira inermis Stem Bark
Abstract
Inflammation is a prominent factor implicated in many diseases. Cancer being one of such disease is the second leading cause of death globally. In this study, the antioxidant activity of different extracts of Andira inermis stem bark was investigated. Further, the ethyl acetate extract was studied for anti-inflammatory and anticancer activities. Successive extraction of A. inermis stem bark with different solvents of increasing polarity gave significantly (p < 0.05) different extracts yields between 3.94 g (1.31%) to 21.66 g (7.22%). Ethyl acetate had a significant amount of total phenolics (133.28 ± 3.20 mg GAE/g) and flavonoids (117 ± 2.96 mg QE/g) when compared to other extracts. The ethyl acetate extract showed significant antioxidant activity using DPPH and ABTS assays with IC50 of 56.11 μg/mL and 35.13 μg/mL respectively. Carrageenan administration resulted in a significant increase in the paw thickness of rats compared to control. Ethyl acetate extract treatment gave a significant reduction in paw size from 1st to 5th hour compared to the negative control group. Treatment of HepG-2 and MCF-7 cell lines with different concentrations of ethyl acetate extract produced IC50 values of 247.26 μg/mL and 365 μg/mL respectively. The antioxidant, anti-inflammatory and anticancer activities of ethyl acetate extract were evidently due to the high total phenolics and flavonoids. The ethyl acetate extract of A. inermis could be used as a potential source of antioxidant, anti-inflammatory, and anticancer agents.
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