Hypolipidemic effect of Clerodendrum violaceum methanol leaf extract in mice


  • Hauwa A. Zailani Department of Biochemistry Author https://orcid.org/0000-0003-2930-430X
  • Elizabeth A. Balogun Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria,Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria Author
  • Joseph O. Adebayo Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria Author https://orcid.org/0000-0003-3151-2247


Clerodendrum violaceum, cardiovascular, risk factors, leaf extract


Effects of methanol leaf extract of Clerodendrum violaceum on selected indices of cardiovascular disease in mice were evaluated. Six groups of ten mice each were used. Groups B-F received orally 31.25, 62.5, 125, 250 and 500 mg/kg body weight of methanol leaf extract of Clerodendrum violaceum respectively while group A received 5% DMSO (control). Half of the animals in each group were sacrificed after 14 days of administration while the remaining half were sacrificed after 28 days of administration. Selected indices of cardiovascular disease were then determined. At the end of 14 days, serum concentrations of total cholesterol, triglyceride and LDL-c, with the atherogenic index were all significantly decreased (p<0.05) at higher doses compared to control; however, serum HDL-c concentration was significantly increased (p<0.05). A similar pattern was observed for these parameters after 28 days of administration. There was significant decrease (p<0.05) in AST activity at the highest dose of the extract in heart and serum and significant increase (p<0.05) in serum AST at other doses on days 14 and 28. Creatine kinase activity was significantly increased (p<0.05) at all doses of the extract in heart and serum on day 14 compared to control but was reversed back to the range of control on day 28. The extract at all doses did not cause any significant change (p>0.05) in serum lipoprotein (a) concentration on days 14 and 28 compared to controls. The results suggest that methanol leaf extract of Clerodendrum violaceum may not predispose subjects to cardiovascular diseases.


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Author Biographies

  • Elizabeth A. Balogun, Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria,Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria

    Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria

  • Joseph O. Adebayo, Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria

    Department of Biochemistry, University of Ilorin, Ilorin, Kwara State, Nigeria


Abolaji, A. O., Adebayo, A. H., & Odesanmi, O. S. (2007). Effect of ethanolic extract of Parinari polyandra (Rosaceae) on serum lipid profile and some electrolytes in pregnant rabbits. Research Journal of Medicinal Plants, 1, 121-127.

Adebayo, J. O., Yakubu, M. T., Egwim, C.E., & Owoyele, B. O. (2003). Effect of ethanolic extract of Khaya senegalensis stem bark on some biochemical parameters of rat kidney. Journal of Ethnopharmacology, 88, 69-72.

Adebayo, J. O., Adesokan, A. A., Olatunji, L. A., Buoro, D. O., & Soladoye, A. O. (2005). Effect of ethanolic extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biokemistri, 17, 45-50.

Adebayo, J. O., Akinyinka, A. O., Odewole, G. A., & Okwusidi, J. I. (2007). Effect of caffeine on the risk of coronary heart disease- A re-evaluation. Indian Journal of Clinical Biochemistry, 22(1), 29-32.

Adebayo, J. O., Igunnu, A., Arise, R. O., & Malomo, S. O. (2011). Effects of co-administration of artesunate and amodiaquine on some cardiovascular disease indices of rats. Food and Chemical Toxicology, 49, 45-48.

Adebayo, J. O., Balogun, E. A., & Zailani, A. H. (2022). In vitro and in vivo antiplasmodial activities and cytotoxicities of Clerodendrum violaceum leaf extracts. African Journal of Biomedical Research, 25(2), 249-255.

Allain, C.C., Poon, L., Chan, S.G., Richmond, W., & Fu, P. (1974). Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20, 470.

Al-Snafi, A. E. (2017). Medicinal plants for prevention and treatment of cardiovascular diseases - A review. IOSR Journal of Pharmacy, 7(4), 103-163.

Ashafa, A. O. T., Yakubu, M. T., Grierson, D. S., & Afolayan, A. J. (2009). Effects of aqueous extract from the leaves of Chrysocomaciliata L on some biochemical parameters of Wistar rats. African Journal of Biotechnology, 8(8), 1425-1430.

Afsheen, N., Rehman, K., Jahan, N., Ijaz,M., Manzoor, A., Khan,K. M., & Hina, S. (2018). Cardioprotective and metabolomic profiling of selected medicinal plants against oxidative stress. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2018/9819360.

Bachorik, P.S., Walker, R., Brownell, K.D., Stunkard, A.J., & Kwiterovich, P.O. (1980). Determination of high density lipoproteincholesterol in stored human plasma. Journal of Lipid Research, 21, 608- 616.

Bailey, S. A., Zidell, R. H., & Perry, R. W. (2004). Relationships between organ weight and body/brain weight in the rat: What is the best analytical endpoint. Toxicologic Pathology, 32, 448–466.

Baloch, S., Gachal, G. S., & Memon, S. A. (2010). Investigation of creatine phosphokinase (CPK) concentration in serum of malaria patients. Sindh University Research Journal (Science Series), 42(2), 71-72.

Balogun, E.A., Adebayo, J.O., Zailani, A.H., Kolawole, O.M., & Ademowo, O.G. (2010). Activity of ethanolic extract of Clerodendrum violaceum leaves against Plasmodium berghei in mice. Agriculture and Biology Journal of North America, 1, 307-312.

Balogun, E.A., Zailani A. H., & Adebayo, J. O. (2014). Augmentation of antioxidant system: Contribution to antimalarial activity of Clerodendrum violaceum leaf extract. CELLMED, 4(4), 26-31.

Chia, B. C. (1991). Cholesterol and coronary artery disease- issue in the 1990’s. Singapore Medical Journal, 32, 29-294.

Di Witt, C., & Trendelenburg, J. (1982). Quantitives determination of creatine kinase in serum. Clinical Chemistry and Clinical Biochemistry, 20, 235.

Eran, B.A., Noah, S., Lee, H. G., Kamer, M., Suha, O., & Elad, S. (2016). Potential risks associated with traditional herbal medicine use in cancer care: A study of Middle Eastern oncology health care professionals. Cancer, 122, 598–610.

Erukainure, O. L., Narainsprasad, N., Singh, M., Olakunle, S., & Islam, M. S. (2018). Clerodendrum volubile inhibits key enzymes linked to type 2 diabetes but induces cytotoxicity in human embryonic kidney (HEK 293) cells via exacerbated oxidative stress and pro inflammation. Biomedicine and Pharmacotherapy, 106, 114-1152.

Friedwald, W. T., Levey, R. I., & Friedrickson, D. S. (1972). Estimation of the concentration of LDL in plasma without the use of preparative concentrating. Clinical Chemistry, 18, 499-502.

Gornall, A. G, Bardawill, C. T., & David, M. M. (1949). Determination of serum protein by means of Biuret reaction. Journal of Biological Chemistry, 177, 751.

Jacobs, N. J., & VanDemark, P. J. (1960). Enzymatic determination of serum triglyceride. Archives of Biochemistry and Biophysics, 88, 250-255.

Krause, W. J. (2001). The art of examining and interpreting histologic preparations. A student handbook. Partheton Publishing group, U.K. Pp 9-10.

Lazic, S. E., Semenova, E., & Williams, D. P. (2020). Determining organ weight toxicity with Bayesian causal models: Improving on the analysis of relative organ weights. Scientific Reports, 10, 6625. doi: 10.1038/s41598-020-63465-y.

Marcovina, S. M., Albers, J. J., Wijsman, E., Zhang, Z., Chapman, N. H., & Kennedy, H. (1996). Differences in lipoprotein (a) and apo(a) polymorphs between black and white Americans. Journal of Lipid Research, 37, 2569-2585.

Nath, P., & Yadav, K.A. (2015). Acute and sub-acute oral toxicity assessment of the methanolic extract from leaves of Hibiscus rosa-sinensis L. in mice. Journal of Intercultural Ethnopharmacology, 4, 70–73.

Nicholls, S. J., & Nelson, A. J. (2019). HDL and Cardiovascular disease. Pathology, 51(2), 142-147.

Nordestgaard, B. G., Chapman, M. J., Ray, K., Borén, J., Andreotti, G. F., Watts, H., Ginsberg, P., Amarenco, A. L., Catapano, O. S., Descamps, E., Fisher, P. T., Kovanen, J. A., Kuivenhoven, P., Lesnik, L., Masana, Z., Reiner, M. R., Taskinen, L., Tokgözoglu, A., & Tybjærg-Hansen, A. (2010). Lipoprotein (a) as a cardiovascular risk factor: current status. European Heart Journal, 31(23), 2844-2853.

Olatunji., L. A., Adebayo, J. O., Oguntoye, O. B., Olatunde, N. O., Olatunji, V. A., & Soladoye, A.O. (2005). Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipid and haematological variables in Rats. Pharmaceutical Biology, 43(5), 471-474.

Porwal, M., Ali Khan, N., & Maheshwari, K. K. (2017). Evaluation of acute and subacute oral toxicity induced by ethanolic extract of Marsdenia tenacissima leaves in experimental rats. Scientia Pharmaceutica, 85, 29. doi:10.3390/scipharm85030029.

Rafiee, L., Keshvari, M., Atar, A. M., Hamidzadeh, Z., Dashti, G. R., Kopaei, M. R., & Asgary, S. (2017). Effect of Gundelia tournefortii L. on some cardiovascular risk factors in an animal model. Journal of Herbmed Pharmacology, 6(4), 191-195.

Razmpoosh, E., Abdollahi, S., & Mousavirad, M. (2022). The effects of olive leaf extract on cardiovascular risk factors in the general adult population: a systematic review and meta-analysis of randomized controlled trials. Diabetology and Metabolic Syndrome, 14, 151. https://doi.org/10.1186/s13098-022-00920-y

Reitman, S., & Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. The American Journal of Clinical Pathology, 28(1), 56–63.

Remya, K., Kaushik, C., & Musuvathi, M. B. (2021). Hepatoprotective and antioxidant capacity of Clerodendrum paniculatum flower extract against carbon tetrachloride-induced hepatotoxicity in rats. ACS Omega, 6(40), 26489-26498.

Roth, G. A., Mensah G. A., & Fuster, V. (2020). The global burden of cardiovascular diseases and risks: a compass for global action. Journal of the American College of Cardiology, 76, 2980-2981.

Saikia, H., & Lama, A. (2011). Effect of Bourgainvillea spectabilis leaves on serum lipids in albino rats fed with high fat diet. International Journal of Pharmaceutical Sciences and Drug Research, 3(2), 141-145.

Shendge, A. K., Basu, T., & Mandal, N. (2021). Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma. Indian Journal of Pharmacology, 53(5), 377-383.

Shrivastatva, N., & Patel, T. (2007). Clerodendrum and Healthcare: An overview Part II Phytochemistry and Biotechnology. Medicinal and Aromatic Plant Science and Biotechnology, 42, 209-217.

Tiwari, R. K., Chanda, S. M. U., Singh, M., & Agarwal, S. (2021). Anti-inflammatory and anti-arthritic potentials of standardized extract of Clerodendrum serratum (L). Moon. Frontiers in Pharmacology, 12, 629607.

Tsao, C., Aday, A. W., Beaton, A. Z., Bittencourt, M., Boehme, A. K., Carson, A. et al., (2022). Heart Diseases and Stroke Statisitics-2022 update: A report from the American Heart Association. Circulation, 45, e153-e639

Vaduganathan, M., Mensah, G., Turco, J. V., Fuster, V., & Roth, A. G. (2022). The global burden of cardiovascular diseases and risk. Journal of the American College of Cardiology, 80(25), 2361–2371. https://doi.org/10.1016/j.jacc.2022.11.005

Wright, P. J. Walker, S., & Plummer, D.T. (1972). Enzymes in rat urine alkaline phosphatase. Enzymologia, 42, 317-327.

Yakubu, M. T., Adebayo, J. O., Egwim, E. C., & Owoyele, V. B. (2005). Increased liver alkaline phosphatase and amino transferase activities following administration of ethanolic extract of Khaya senegalensis stem bark torats. Biokemistri, 17(1), 27-32.

Yakubu, M. T., Akanji, M. A., & Oladiji, A. T. (2008). Alterations in serum lipid profile of male rats by oral administration of aqueous extract of Fadogia agrestis stem. Research Journal of Medicinal Plants, 2(2), 66-73.

Zhang, Y., Pletcher, M., J., & Vittinghoff, E. (2021). Association between cumulative low-density lipoprotein cholesterol exposure during young adulthood and middle age and risk of cardiovascular events. JAMA Cardiology, 6, 1406-1413.



How to Cite

Hypolipidemic effect of Clerodendrum violaceum methanol leaf extract in mice. (2023). Nigerian Journal of Biochemistry and Molecular Biology, 38(1), 33-42. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/120

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