In vivo and in vitro anti-diabetic activity of extracts of Anthoclestia vogelii Planch and isolation of Decussatin, a new α-amylase inhibitor from its stem bark and leaves




The aqueous methanol crude extracts of Anthocleista vogelii, Planch were investigated for their anti-diabetic activities in normoglycaemic and alloxan-induced diabetic rats. Bioassay guided fractionation in vitro was used to investigate the α-amylase inhibitory activities. The leaves and stem bark crude extracts at 1 g/kg body weight (BW) gave 60.35 % and 69.68 % (p < 0.05) peak reduction in blood glucose levels on day-7 and day-5 respectively, which was higher than the 58.43 % for the reference group, glibenclamide given on day-7. Decussatin, a xanthone at 1 mg/mL with a 78.0 % (p < 0.001) α-amylase inhibition was isolated with comparable anti-diabetic activity to acarbose, a known synthetic α-glucosidase drug, which gave a 54.9 % inhibition at the same concentration. Spectral characterization of the isolated compound using IR, GC-MS, and NMR established the structure of the compound to be decussatin. In addition, computational calculations further confirmed the structure as decussatin with spectroscopic data that matched the experiment. The anti-diabetic activity as revealed by the results further validates, scientifically, the traditional use of the plant in managing diabetes. As such, we provide evidence of a probable mode of operation by α-amylase inhibition due to the presence of decussatin isolated from the leaves and stem bark.


Abdel-Barry, J. A., Abdel-Hassan, I. A., & Al-Hakiem, M. H. (1997). Hypoglycaemic and antihyperglycaemic effects of Trigonella foenum-graecum leaf in normal and alloxan induced diabetic rats. Journal of ethnopharmacology, 58(3), 149-155.

Abuh, F. Y., Wambebe, C., Rai, P. P., & Sokomba, E. N. (1990). Hypoglycaemic activity of Anthocleista vogelii (Planch) aqueous extract in rodents. Phytotherapy Research, 4 (1), 20-24.

American Diabetes Association (2010). Diagnosis and classification of diabetes mellitus. Diabetes care, (Suppl 1), S62–S69.

Alaribe, C. S. A., Coker, H. A. B., Shode F.O., Ayoola G., Adesegun, S. A., Ezenwa, J. B. I., & Anyim, C. A. (2012). Antiplasmodial and Phytochemical Investigations of Leaf Extract of Anthocleista vogelii (Planch). Journal of Natural Products, 5, 60-67.

Ampofo, O. (1977). Some clinical observations of the treatment of selected diseases by herbal preparations. Ile-Ife: Drug Research Unit, University of Ife. 35-45.

Benfield, P. (1951). Enzymes of starch degradation and synthesis. In: Advances in enzymology XII, (F. Noed, ed.), 379. Interscience, New York.

Burkill, H. M. (1995). The Useful Plants of West Tropical Africa. 2nd ed. Volume 3. Botanical Gardens, London. 124-126.

Chen, L., Magliano, D. J., & Zimmet, P. Z. (2011). The worldwide epidemiology of type 2 diabetes mellitus--present and future perspectives. Nature reviews. Endocrinology, 8(4), 228–236.

Evans J. L. and Rushakoff, R. J. (2007). Oral pharmacological agents for type 2 diabetes: sulfonylureas, meglitinides, metformin, thiazolidinediones, ????-glucosidase inhibitors, and emerging approaches. In: Chapter 14. Endo Carbohydrate Metabolism and diabetes mellitus, (I.D. Goldfine, R.J. Rushakoff, ed.)

Frisch, M.J. Gaussian 09. Revision E.01, (M.J. Frisch, et al. Gaussian, Inc., Wallingford CT, 2013).

Handa, S. S., Khanuja, S. P. S., Longo, G., and Rakesh, D. D. (2008). Extraction technologies for medicinal and aromatic plants, (1st Ed.), no. 66, Italy: United Nations

Industrial Development Organisation and the International Centre for Science and High Technology International Diabetes Federation (2017). Diabetes Atlas.

Jegede, I. A., Ibrahim, J. A. & Kunle, O. F. (2011). Phytochemical and pharmocognostic studies of the leaf and stem-bark of Anthocleista vogelii (Planch). Journal of Medicinal Plants Research, 5(26), 6136-6139.

Kang, W., Song, Y. & Gu, X. (2012). Alpha-glucosidae inhibitory in vitro and antidiabetic activity in vivo of Osmanthus fragans. Journal of Medicinal Plants Research, 6(14), 2850-2856.

Kwon, Y. I., Apostolidis, E., Kim, Y. C., & Shetty, K. (2007). Health benefits of traditional corn, beans, and pumpkin: in vitro studies for hyperglycemia and hypertension management. Journal of medicinal food, 10(2), 266–275.

Lebovitz H. E. (1997). alpha-Glucosidase inhibitors. Endocrinology and metabolism clinics of North America, 26(3), 539–551.

Li Y., Wen, S., Kota, P. B., Peng, G., Li Q. G., Tamahara, J. & Roufogalis, D. B. (2005). Punica granatum flower extract, a potent alpha-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. Journal of Ethnopharmacology, 99(2), 239-244.

Madingou, N. O. K., Souza, A., Lamidi, M., Mengome, L. E., Mba, C. E. M., Bading, B., J. Mavoungou, J., & Traore, A. S. (2012). Study of medicinal plants used in the management of cardiovascular diseases at Libreville (Gabon): an ethnopharmacological approach. International Journal of Pharmaceutical Sciences and Research, 3 (1), 111-119.

Mbouangouere, R. N., Tane, P., Ngamga, D., Khan, S. N. Choudhary, M. I., & Ngadjui, B. T. (2007). A new steroid and glucosidase inhibitors from Anthocleista schweinturthi. Research Journal of Medicinal Plants, 1 (3), 106-111.

Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428.

Maitra, A., & Abbas, A. K. (2005). Endocrine system. In: Kumar, V., Fausto, N., & Abbas A. K. (eds). Robbins and Cotran Pathologic basis of disease (7th ed). Philadelphia, Saunders; 1156-1226.

Muhammed, M., & Lakshimi, P. (2005). Diabetes Management: The therapeutic role of Ayurvedic herbs. 1-10.

Oboh, G., Akinyemi, A. J., and Ademiluyi, A. O. (2012). Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfairia occidentalis (fluted pumpkin) leaf. Asian Pacific Journal of Tropical Biomedicine, 2 (9), 733–738.

Okorie, D. A. (1976). A new phthalide and xanthones from Anthoclesista djalonensis and Anthocleista vogelii. Phytochemistry, 15, 1799-1800.

Olubomehin, O. O., Abo, K. A., & Ajaiyeoba, E. O. (2013). Alpha–amylase inhibitory activity of two Anthocleista species and in vivo Rat model anti-diabetic activities of Anthocleista djalonensis extracts and fractions. Journal of Ethnopharmacology, 146(3), 811-814.

Osadebe, P. O., Uzor, P. F., Omeje, E.O., Agbo, M. O., & Obonga, W. O. (2014). Hypoglycemic activity of the extract and fractions of Anthocleista vogelii (Planch) stem bark. Tropical Journal of Pharmaceutical Research, 13 (9), 1437-1443.

Pubchem., Accessed date: 17/12/2019.

Qamar, F., Afroz, S., Feroz, Z., Siddiqui, S., & Ara, A. (2011). Evaluation of hypoglycaemic effect of Eassia italica. Journal of Basic and Applied Sciences, 7 (1), 61-64.

Rahimzadeh, M., Jahanshahi, S., Moein, S., & Moein, M. R. (2014). Evaluation of alpha-amylase inhibition by Urticadioica and Juglansregia extracts. Iranian Journal of Basic Medical Sciences, 17 (6), 465–469.

Silva, L. F. R., Lima E. S., de Vasconcellos, M. C., Aranha, E. S. P., Costa, D. S., Elba, E. V., de Morais, S. K. R., Alecrim, M. C., Nunomura, S. M., Struwe, L., Valter Ferreira de Andrade-Neto, V. F., & Pohlit, A. M. (2013). In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the Amazonian plant Tachia grandiflora (Gentianaceae). Memórias do Instituto Oswaldo Cruz, 108(4), 501–507.

Sim, L., Jayakanthan, K., Mohan S., Nasi, R., Johnston, B. D., Pinto, B. M., & Rose, D. R. (2010). New glucosidase inhibitors from an ayurvedic herbal treatment for type 2 diabetes: structures and inhibition of human intestinal maltase glucoamylase with compounds from Salacia reticulate. Biochemistry, 49 (3), 443–451.

Sunday, R. M., Ilesanmi, O. R., Obuotor, E. M., Ibeh, A. J., & Ayannuga, O. A. (2015). Anti-diabetic effect of Anthocleista vogelii aqueous root extract in Streptozotocin-induced diabetic rats. Ife Journal of Science, 17(3), 755-763.

Sunday, R. M., Ilesanmi, O. R., & Obuotor, E. M. (2016). Anti-diabetic effect of Anthocleista vogelii ethanolic root extract in alloxan-induced diabetic rats. Research Journal of Medicinal Plant, 10(1), 79-88.

Thilagam, E., Parimaladevi, B., Kumarappan, C., & Chandra-Mandal, S. (2013). Alpha-glucosidase and a-amylase inhibitory activity of Senna surattensis. Journal of Acupuncture and Meridian Studies, 6(1), 24-30.

Zheng, Y., Ley, S. H., & Hu, F. B. (2018). Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature Reviews. Endocrinology, 14(2), 88-98.



How to Cite

Olubomehin, O. O., Faponle, A. S., Ajaiyeoba, E. O., & Abo, K. A. (2022). In vivo and in vitro anti-diabetic activity of extracts of Anthoclestia vogelii Planch and isolation of Decussatin, a new α-amylase inhibitor from its stem bark and leaves. Nigerian Journal of Biochemistry and Molecular Biology, 37(1), 9–16.



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