Phytochemical Analysis of Allium cepa and Allium fistulosum by Gas Chromatography-Mass Spectrometry Analysis: A Comparative Study

Authors

  • Gabriel O. Ajayi Department of Biochemistry, College of Basic and Applied Sciences, Mountain Top University, Prayer City, Ogun State, Nigeria Author
  • Mushafau A. Akinsanya Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria Author https://orcid.org/0000-0001-8842-1069

Keywords:

GC-MS, Allium cepa, Allium fistulosum, Bioactive components

Abstract

Allium cepa and Allium fistulosum are herbs used in many parts of the world as spice, food and medicine. To evaluate the phytochemical components, the aqueous extracts of the leaves of Allium fistulosum and fleshy leaves of Allium cepa were used. The phytochemical components of both plants were evaluated and compared using GC-MS analysis. There were ten (10) bioactive components in Allium cepa revealed by GC-MS analysis which are majorly 16-Deoxo-5,6-dihydrokryptogenin diformate (Retention Time (RT): 32.245 minutes, Molecular. Weight (mol. wt).: 474, yield: 30.636%), acetic acid, dichloro- (RT: 3.280-3.349, mol. wt.: 128, yield: 12.710%), 9-eicosene, (E)- (RT: 33.033, mol. wt.: 280, yield: 7.139%) and others with less than 6% yield. Whereas, in Allium fistulosum, five (5) bioactive components were present and D-limonene (RT: 6.694, mol. wt.: 136, yield: 98.942%) as the main component. acetic acid, dichloro- was common in both plants but higher in Allium cepa (12.710%) than Allium fistulosum (0.484%). It is therefore concluded that A. cepa and A. fistulosum possess different and diverse phytochemical compounds that could be responsible for their biological activities.

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References

Ajayi, G. O., Akinsanya, M. A., Agbabiaka, A. T., Oyebanjo, K. S., Hungbo, T. D. and Olagunju, J. A. (2019). D-Limonene: A major bioactive constituent in Allium fistulosum identified by GC-MS analysis. Journal of Phytopharmacology, 8(5), 257-259. http://doi.org/10.31254/phyto.2019.8509

Ajayi, G. O., Olagunju, J. A., Ademuyiwa, O. and Martins, O. C. (2011). Gas chromatography-mass spectrometry analysis and phytochemical screening of ethanolic root extract of Plumbago zeylanica, Linn. Journal of Medicinal Plants Research, 5(9), 1756-1761.

Belakhdar, G., Benjouad, A. and Abdennebi, E. H. (2015). Determination of some bioactive chemical constituents from ThesiumhumileVahl. Journal of Materials and Environmental Science, 6(10), 2778-2783.

Benmalek, Y., Yahia, O. A., Belkebir, A. and Fardeau, M. L. (2013). Antimicrobial and anti-oxidant activities of Illicium verum, Crataegusoxyacanthasspmonogyna and Allium cepa red and white varieties. Bioengineered, 4(4), 244-248.

Chen, J. H., Chen, H. I., Wang, J. S., Tsai, S. J. and Jen, C. J. (2000). Effects of welsh onion extracts on human platelet function in vitro. Life Sciences, 66(17), 1571-1579.

Dawang, S. N., Affiah, D. U., Lanka, N. J. and Fannap, L. M. (2016). Preliminary checklist of spices and culinary herbs sold in Jos, Plateau State, Nigeria. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 11(4), 24-29. http://doi.org/10.9790/3008-1104032429

De-Fatima, A., Modolo, L. V., Conegero, L. S., Pilli, R. A., Ferreira, C. V., Kohn, L. K. and De-Carvalho, J. E. (2006). Lactones and their derivatives: biological activities, mechanisms of action and potential leads for drug design. Current Medicinal Chemistry, 13(28), 3371-3384.

Dinesh, K. G., Karthik, M. and Rajakumar, R. (2018). GC-MS analysis of bioactive compounds from ethanolic leaves extract of Eichhorniacrassipes (Mart) Solms. and their pharmacological activities. The Pharma Innovation Journal, 7(8), 459-462.

Erasto, P. and Viljoen, A. M. (2008). Limonene - A Review: Biosynthetic, Ecological and Pharmacological Relevance. Natural Product Communications, 3(7), 1193-1202.

Fedel-Miyasato, L. E. S., Formagio, A. S. N., Auharek, S. A., Kassuya, C. A. L., Navarro, S. D., Cunha-Laura, A. L., ... & Oliveira, R. J. (2014). Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: a comparative study. Genetic Molecular Research, 13(2), 3411-3425.

Ifesan, B. O. T. (2017). Chemical composition of onion peel (Allium cepa) and its ability to serve as a preservative in cooked beef. International Journal of Science and Research Methodology, 7(4), 25-34.

Kadan, S., Saad, B., Sasson, Y. and Zaid, H. (2013). In vitro evaluations of cytotoxicity of eight antidiabetic medicinal plants and their effect on GLUT4 translocation. Evidence Based Complementary and Alternative Medicine, 2013, 549345.

Kaiser, P., Youssouf, M.S., Tasduq, S.A., Singh, S., Sharma, S.C., Singh, G.D., Gupta, V.K., Gupta, B.D. and Johri, R.K., 2009. Anti-allergic effects of herbal product from Allium cepa (bulb). Journal of Medicinal Food, 12(2), 374-382.

Keller, E. R. J., Blattner, F. R., Fritsch, R., Pistrick, K., Senula, A. and Zanke, C. D. (2012). The Genus Allium in the Gatersleben plant collections–progress in germplasm preservation, characterization and phylogenetic analysis. Acta Horticulturae, 969, 273-287.

Kris-Etherton, P. M., Hecker, K. D., Bonanome, A., Coval, S. M., Binkoski, A. E., Hilpert, K. F., ... &Etherton, T. D. (2002). Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. The American Journal of Medicine, 113(9), 71-88.

Kumar, K. E., Harsha, K. N., Sudheer, V. and Nelli, G. B. (2013). In vitro antioxidant activity and in vivo hepatoprotective activity of aqueous extract of Allium cepa bulb in ethanol induced liver damage in Wistar rats. Food Science and Human Wellness, 2, 132-138.

Kumari, K and Augusti, K. T. (2007). Lipid lowering effect of S-methyl cysteine sulfoxide from Allium cepa Linn in high cholesterol diet fed rats. Journal of Ethnopharmacology, 109(3), 367-371.

Kuppuswamy, K. M., Jonnalagadda, B. and Arockiasamy, S. (2013). GC-MS analysis of chloroform extract of Croton bonplandianum. International Journal of Pharmacology and Biological. Sciences, 4(4), 613-617.

Lai, W. W., S. C. Hsu, Chueh, F. S., Chen, Y. Y., Yang, J. S., Lin, J. P., Lien, J. C., Tsai, C. H. and Chung, J. G. (2013). Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-κB and matrix metalloproteinase-2/-9 signaling pathways. Anticancer Research, 33(5), 1941-1950.

Lee, J. B., Miyake, S., Umetsu, R., Hayashi, K., Chijimatsu, T and Hayashi, T. (2012). Anti-influenza A virus effects of fructan from Welsh onion (Allium fistulosum L.). Food Chemistry, 134(4), 2164-2168.

Mamani, R. and Alhaji, N. M. (2019). GC-MS analysis of phytocomponents in methanolic extract of Coleus aromaticus. Journal of Pharmacognosy and Phytochemistry, 8(4), 106-109.

Mantawy, M. M., Ali, H. F. and Rizk, M. Z. (2011). Therapeutic effects of Allium sativum and Allium cepa in Schistosoma mansoni experimental infection. Revista do Instituto de Medicina Tropical de São Paulo (Journal of the Institute of Tropical Medicine of São Paulo), 53, 155-163.

Marchese, A., Orhan, I. E., Daglia, M., Barbieri, R., Lorenzo, A. D., Nabavi, S. F., Gortzi, O., Izadi, M. and Nabavi, S. M. (2016). Antibacterial and antifungal activities of thymol: a brief review of the literature. Food Chemistry, 210, 402-414. http://dx.doi.org/10.1016/j.foodchem.2016.04.111

Mootoosamy, A. and Mahomoodally, M. F. (2014). Ethnomedicinal application of native remedies used against diabetes and related complications in Mauritius. Journal of Ethnopharmacology, 151(1), 413-44.

Nasri, S. and Anoush, M. (2012). Evaluation of analgesic and anti-inflammatory effects of fresh onion juice in experimental animals. African Journal of Pharmacy and Pharmacology, 6(23), 1679-1684.

Oloyede, A., Okpuzor, J. and O. Omidiji, O. (2009). Cytological and toxicological properties of a decoction used for managing tumors in Southwestern Nigeria. Pakistan Journal of Biological Sciences, 12(4), 383-387.

Onion (2020). History. Available on https://www.onions-usa.org/all-about-onions/history-of-onions/ [Last retrieved on September 19, 2020]

Onwuamah, C. K., Ekama, S. O., Audu, R. A., Ezechi, O. C., Poirier, M. C. and Odeigah, P. G. (2014). Exposure of Allium cepa root cells to zidovudine or nevirapine induces cytogenotoxic changes. PLoS One, 9(3), e90296.

Park, I. K. and Shin, S. C. (2005). Fumigant activity of plant essential oils and components from garlic (Allium sativum) and clove bud (Eugenia caryophyllata) oils against the Japanese termite (Reticulitermes speratus Kolbe). Journal of Agricultural and Food Chemistry, 53(11), 4388-4392.

Perez, O. A., Viera, M. H., Patel, J. K., Konda, S., Amini, S., Huo, R., et al. (2010). A comparative study evaluating the tolerability and efficacy of two topical therapies for the treatment of keloids and hypertrophic scars. Journal of Drugs in Dermatology, 9(11), 5184-5188.

Porchezhian, E. and Ansari, S. H. (2000). Effect of liquid extract from fresh Abutilon indicum leaves and Allium cepa bulbs on paracetamol and carbon tetrachloride induced hepatotoxicity. Pharmazie, 55, 702-703.

Rivas da Silva, A. C., Lopes, P. M., Barros de Azevedo, M. M., Coata, D. C., Alviano, C. S. and Alviano, D. S. (2012). Biological activities of α-pinene and β-pinene enantiomers. Molecules, 17(6), 6305-6316.

Russo, E. B. (2011). Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects. British Journal of Pharmacology, 163(7), 1344-1364. https://doi.org/10.1111/j.1476-5381.2011.01238

Sakakibara, H., Yoshino, S., Kawai, Y. and Terao, J. (2008). Antidepressant-like effect of onion (Allium cepa L.) powder in a rat behavioral model of depression. Bioscience, Biotechnology and Biochemistry, 72(1), 94-100.

Salami, H. A., John, A. I. and Ekanem, A. U. (2012). The effect of aqueous preparation of Allium Cepa (onion) and Allium Sativa (garlic) on erythrocyte osmotic fragility in wistar rats: in vivo and in vitro studies. Nigerian Journal of Physiological Sciences, 27(1), 029-034.

Sang, S., Lao, A., Wang, Y., Chin, C. K., R. T. Rosen, R. T. and Ho, C.T. (2002). Antifungal constituents from the seeds of Allium fistulosum L. Journal of Agricultural and Food Chemistry, 50(22), 6318-6321.

Shaik, Y.B., Castellani, M.L., Perrella, A., Conti, F., Salini, V., Tete, S., Madhappan, B., Vecchiet, J., De Lutiis, M.A., Caraffa, A. and Cerulli, G., 2006. Role of quercetin (a natural herbal compound) in allergy and inflammation. Journal of Biological Regulators and Homeostatic Agents, 20(3-4), 47-52.

Singh, B. K. and Ramakrishna, Y. (2017). Welsh Onion (Allium fistulosum L.): A Promising Spicing-Culinary Herb of Mizoram. Indian Journal of Hill Farming, 30(2), 201-208.

Sung, Y. Y., Kim, D. S., Kim, S. H. and Kim, H. K. (2018). Aqueous and ethanolic extracts of welsh onion, Allium fistulosum, attenuate high-fat diet-induced obesity. BMC Complementary and Alternative Medicine, 18(105), 1-11. http://doi.org/10.1186/s12906-018-2152-6

Sung, Y. Y., Kim, S. H., Kim, D. S., Park, S. H., Yoo B. W. and Kim, H. K. (2014). Nutritional composition and anti-obesity effects of cereal bar containing Allium fistulosum (welsh onion) extract. Journal of Functional Foods, 6, 428-437.

Tataranni, T. and Piccoli, C. (2019). Dichloroacetate (DCA) and Cancer: An Overview towards Clinical Applications. Hindawi Oxidative Medicine and Cellular Longevity, 2019, 1-14. https://doi.org/10.1155/2019/8201079

Upadhyay, R. K. (2016). Nutraceutical, pharmaceutical and therapeutic uses of Allium cepa: A review. International Journal of Green Pharmacy, 10(1), 46-64.

Wananukul, S., Chatpreodprai, S., Peongsujarit, D. and Lertsapcharoen, P. (2013). A prospective placebo-controlled study on the efficacy of onion extract in silicone derivative gel for the prevention of hypertrophic scar and keloid in median sternotomy wound in pediatric patients. Journal of Medical Association of Thailand, 96(11), 1428-1433.

Yamamoto, Y., S. Aoyama, S., Hamagushi, N. and Rhi, G. S. (2005). Antioxidative and antihypertensive effects of welsh onion on rats fed with a high-fat high sucrose diet. Bioscience, Biotechnology and Biochemistry, 69(7), 1311-1317.

Published

2023-03-24

How to Cite

Phytochemical Analysis of Allium cepa and Allium fistulosum by Gas Chromatography-Mass Spectrometry Analysis: A Comparative Study. (2023). Nigerian Journal of Biochemistry and Molecular Biology, 38(2), 73-82. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/143