Phytochemical Composition, Antioxidant and Antitrypanosomal Properties of Leaves and Stem Bark Extract of Detarium microcarpum: An in vitro study


  • Jonathan U. Emohchonne Federal University
  • Uche S. Ndidi
  • Aishat S. Kashim
  • Ibrahim Sofiyullahi
  • Sani Binta
  • Ismail Abdulmalik
  • Awai J. Kagbu
  • Abdulyekin Jamila



Trypanosomiasis is a major disease in the Africa continent that affects both humans and livestock. This study investigated the phytochemical composition, antioxidant and antitrypanosomal activities of the extracts from Detarium microcarpum leaves and stem bark. Sequential extraction of the plant samples was done with the solvents of increasing polarity and these extracts were assayed for antioxidant properties, phytochemical constituents, and antitrypanosomal (Trypanosoma congolense and Trypanosoma brucei brucei) activity using standard protocols. The result from the phytochemical studies showed that methanolic extract of the stem bark has the highest flavonoid and phenolic contents (596.88 ± 0.13 mgQE/mg of extract and 367.3 ± 15.84 mg/GAE/mg of extract). The in vitro antioxidant study showed that there was no significant difference in the DPPH scavenging activities of the various extracts compared to ascorbic acid. However, aqueous stem bark extract and methanolic extracts of the leaves and stem bark showed a significant difference in H2O2 scavenging activity when compared to ascorbic acid, with an IC50 value of 1227.04 ± 77.19 μg/mL, 109.61 ± 13.38 μg/mL and 447.40 ± 12.04 μg/mL. From the in vitro antitrypanosomal studies, methanolic leaves extract of D. microcarpum showed an efficient antitrypanosomal effect with complete cessation in motility at 5 min against Trypanosoma congolense, and at 10 min against Trypanosoma brucei brucei at the highest concentration of the extract (20 mg/mL). The result of this study shows that the various extracts of D. microcarpum possess possible inhibitory potential against free radicals in addition to its antitrypanosomal effect.


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How to Cite

Emohchonne, J. U. ., Ndidi, U. S. ., Kashim, A. S. ., Sofiyullahi, I. ., Binta, S. ., Abdulmalik, I. ., Kagbu, A. J. ., & Jamila, A. . (2022). Phytochemical Composition, Antioxidant and Antitrypanosomal Properties of Leaves and Stem Bark Extract of Detarium microcarpum: An in vitro study. Nigerian Journal of Biochemistry and Molecular Biology, 37(3), 158–165.



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