Impact of Some Heavy Metals in Soil on Oxidative Stress Indices and Chlorophyll Levels of Telfaria occidentalis Leaves

Authors

  • Nwaogu, L. A. Department of Biochemistry, School of Science, Federal University of Technology Owerri, Nigeria Author
  • Ibegbulem, C. O. Department of Biochemistry, School of Science, Federal University of Technology Owerri, Nigeria Author
  • Ujowundu, C. O. Department of Biochemistry, School of Science, Federal University of Technology Owerri, Nigeria Author
  • Alozie, S. C. Department of Biochemistry, School of Science, Federal University of Technology Owerri, Nigeria Author

Abstract

The effect of some heavy metals [mercury (Hg), lead (Pb) and cadmium (Cd)] in soil at different concentrations (50, 80 and 100 g/dm3) on oxidative stress indices [glutathione (GSH), ascorbic acid (AA), lactate dehydrogenase (LDH) activity] and levels of chlorophyll from aqueous extract of Telfaria occidentalis leaves was investigated after one hundred and twenty days of exposure. Telfaria occidentalis from soil sample without heavy metal pollution served as control. Results revealed that there was a significant ( p< 0.05) decrease in all these parameters (the concentrations of GSH, AA, activity of LDH and levels of chlorophyll) as the concentration of these metals increases, when compared to the control. The results indicated that these metals at 80 and 100 μg/dm3 after exposure for one hundred and twenty days, induced stress in T. occidentalis leaves.

Downloads

Download data is not yet available.

References

Achuba, F. I. (2011). Effects of refined petroleum products in soil on seedlings of maize (Zea mays) and cowpea (Vigna ungulculata). Ph.D. Thesis, Delta State University, Abraka, Delta State, Nigeria. pp. 284-285.

Ajibola, V. O., Folaranmi, F. M., Fasae, O. A. and Adegoke. D. (2002). Uptake and accumulation of some metals by spinach (Amaranthus tricolor). Journal of Tropical Biosciences. 2: 41-44.

Amadi, B. A., Agomuo, E. U. and Ibegbulem, C. O. (2004). Research methods in Biochemistry. Supreme Publishers, Owerri, Nigeria. pp. 120-134.

Chen, H., Lin, K., Hung, T., Wei, B. I., Hour, T., Yen, M., Pu, Y. and Lin, C. (2010). Terpenoids-induced cell cycle arrest and apoptosis from the stems of Celastrus kusanri associated with reactive oxygen species. Journal of Agriculture and Food Chemistry. 58:3808-3812.

De Fillips, L. F. and Ziegler, H. (1993). Effect of sub lethal concentrations of zinc, cadmium and mercury on the photosynthesis and carbon reduction cycle of Euglena. Journal of Plant Physiology. 142:167-172.

Eja, M. E., Ogri, O. R. and Arikpo, G. E. (2003). Bioconcentration of heavy metals in surface sediments from the great Kwa River Estuary, Calabar, South eastern Nigeria. Journal of Nigerian Environment and Society. 1:247-256.

Esiba, R. O. (1982). Cultivating the fluted pumpkin in Nigeria world crops, March/ April, 70-72.

Gills, E., Lofflers, S., Winnacker, E. I. and Zeak, M. H. (1989). Phytochelatins, the heavy-metal building peptides of plants are synthesized from glutathione by a specific -glutamyl cysteine dipeptidyl transpeptidase ( phytochelatin synthase) . Proceedings of the National Academy of Science, USA. 86: 6838-6842.

He, Z. L., Zhang, M. K., Calvert, D. V., Stoffela, P. J., Yang, X.E. and Ya, S. (2004). Transport of heavy metals in surface run-off from vegetable and citrus fields. American Journal of Soil Science.68:1662-1669.

Hogan, C. M. (2010). Heavy metal. In: Encyclopedia of National Council for Science and the Environment. Monosson, E. and Clereland, C (eds) Washington, DC. pp. 2345-2358.

Jollow, D. J., Michel, J. R, Zampageionic, N. and Gillette, J. R. (1974). Necrosis protective role of glutathione and evidence for 3,4-brombezene oxide as a hepatotoxic metabolites. Pharmacology. 11: 151-169.

Kaiglova, A., Reichrtova, E., Aclameakova, A. and Wsolora, L. (2001). Lactate dehydrogenase activity in human placenta following exposure to environmental pollutants.Physiology Research. 50:525-528.

Keller, T. and Schwager, M. (1977). Air pollution and ascorbic acid. Environmental Journal of Pathology. 7:338-350.

Lacatusa, R. (2003). Application levels of soil contamination and pollution with heavy metals. Environmental Soil Bureau Research Report. No. 4.

Levine, R. C., Gaxland, D. C., Oliver, C. N., Amice, A., Clement, T., Lenz, A. U., Alin, B. W., Shaliel, S. and Stadima, E. R. (1990). Determination of carbonyl concentration in oxidatively modified proteins. Methods in Enzymology. 186:404-478.

Ma, Q. Y., Traina, Q. J., Traina, S. J. and Logan, T. J. (1994). Effect of aqueous Al, Cd Fe II, Ni and Zn on Pb immobilization by hydroxyapatite. Environmental Soil Technology. 28(7): 1219-1228.

McKee, T. and McKee, J. R. (1999). Biochemistry, An Introduction. 2nd edition. pp.132-135.

Misra, V. and Chaturvedi, P. K. (2007). Plant uptake bioavailability of heavy metals from the contaminated soil after treatment with humus soil and hydroxyl apatite. Environmental Monitor and Assessment. 133:169-176

Nkang, A., Omokaro, O., Egbe, A. and Amanke, G. (2003). Variation in fatty acids and proportion during desiccation of Telfaria occidentalis seeds harvested at physiological and agronomic condition. African Journal of Biotechnology. 2:33-39.

Ndiokewere, C. C. and Ezehe, C. A. (1990). The occurrence of heavy metals in the vicinity of industrial complexes in Nigeria. Environmental International. 16:291-295.

Nwaogu, L. A., Agha, N. C., Alisi, C. S. and Ihejirika, C. E. (2011). Investigation on the effect of cassava effluent-polluted soil on germination, emergence and oxidative stress parameters of Telfaria occidentalis. Journal of Biodiversity and Environmental Sciences. 1(6): 104-111.

Nriagu, J. O. (1989). A global assessment of natural sources of atmospheric trace metals. Nature 338:47-49.

Rennenberg, H. (1995). Processes involved in glutathione metabolism. In: Wallsgrove, R.M.(ed). amino acids and their derivatives in higher plants. UK, Cambridge University. pp. 155-177.

Roon, K. P. (2003). Construction and characterization of multiple heavy metal resistant phenol degrading Pseudomonas. Trends in Microbiology and Biotechnology. 13(6): 1001-1007.

Singh, S. N. and Rao, D. N. (1981). Certain responses of wheat plant to cement dust pollution. Environmental Pollution. ( series A). 24:75-81.

Valarmathe, S. and Azariah, J. (2003). Effect of copper chloride on the enzyme activities of the crab (Sesarma quadratum) ( Fabricius). Turkish Journal of Zoology. 27:253-256.

Woodward,R. B., Ayer, W. A. and Beaton, J. M. (1990). The total synthesis of chlorophyll. PDF tetrahedron. 46 (22): 7599-7659.

Zauyah, S., Juliana, B., Noorhafizah, R., Fauziah, C. I. and Rosenami, A. B. (2004). Concentration and speculation of heavy metals in some cultivated and uncultivated utisoils and inceptisoils in Peninsular, Malaysia Super soil, Australian. New Zealand Soil Conference. University of Sydney, Australia.

Published

2012-05-20

How to Cite

Impact of Some Heavy Metals in Soil on Oxidative Stress Indices and Chlorophyll Levels of Telfaria occidentalis Leaves. (2012). Nigerian Journal of Biochemistry and Molecular Biology, 27(1&2), 46-52. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/283