Effect of methanolic extract of Tetracarpidium conophorum (African walnut) seeds on haematological parameters of carbon tetrachloride treated male rats

Authors

  • Uadia, P. O. Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria. Author
  • Oriakhi, K. Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria Author
  • Osemwenkhae, P. O. Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria Author
  • Emokpae, M. A. Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria Author

Abstract

The effect of the methanolic extract of Tetracarpidium conophorum seeds on the haematological parameters of carbon tetrachloride treated male rats was investigated. Thirty two albino rats (170 ±10.0) g were assigned into 8 groups of 4 rats each. Animals in Group I (control) was given distilled water only, while those in groups II, III and IV were administered T. conophorum extract at different concentrations of 250, 500, 750 mg/kg body weight daily for two weeks, thereafter, they were given the extract three times per week for three weeks. Group V received CCl4 only (positive control) at a dose of 0.598g/kg body weight. Groups VI, VII, and VIII animals were given the extract (same doses), for two weeks, then CCl4 was given orally for two weeks, three times per week simultaneously with the extract. The results showed that CCl4 only produced significant decrease (P<0.05) in white blood cell (WBC count), red blood cell (RBC), hematocrit (HCT), platelets, plasma viscosity (PV), fibrinogen (FB), mean cell volume (MCV) and mean cell hemoglobin (MCH) while there was significant increase (P>0.05) in lymphocytes when compared with the control. When the extract was administered alone there was significant increase (P<0.05) in white blood cell, lymphocytes, plasma viscosity, and fibrinogen, while platelets were reduced significantly (P<0.05) when compared with the control. Furthermore, when the extract was administered to CCl4 treated rats, there was significant increase (P<0.05) in WBC, RBC, HCT, PV and FB while platelets, neutrophils were reduced significantly (P<0.05) when compared with the positive control.

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References

Ajaiyeoba, E. O. and Fadare, D. A. (2006). Antimicrobial potential of extracts and fractions of the African walnut Tetracarpidium conophorum. African Journal of Biotechnology 5:2322-2327.

ATSDR "Agency for Toxic Substance and Diseases Registry" (1994). Toxicological profile for carbon tetrachloride, U.S. Department of Health and Human Services, Public Health Service, TP-39/02. Atlanta, CA.

Guo, T. L., McCay, J. A., Brown, R. D., Musgrove, D. L., Germolec, D. R., Butterworth, L., Munson A. E. and White Jr, K. L. (2000). Carbon tetrachloride is immunosuppressive and decreases host resistance to Listeria monocytogenes and Streptococcus pneumoniae in female B6C3F1 mice. Toxicology 154: 85-101.

Herbert, J. R., Hurley, T. G., Olendzki, B. C, Teas, J., Ma, Y. and Ha, J. S. (1998). Nutritional and socioeconomic factors in reaction to prostate cancer mortality: a cross-national study. Journal of National Cancer Institute 90: 1637-1647.

Hutchinson, J. and Dalziel, J. M. (1987). Flora of west Tropical Africa. 2nd Edn., pp. 111- 112.

Ingram, C. I. C (1961). A suggested schedule for rapid investigation of acute haemostatic failure. Journal of Clinical Pathology. 14: 356-360.

Jirova, D., Sperlingova, I., Halaskova, M., Bendova, H. and Dabrowska, L. (1996). Immunotoxic effects of carbon tetrachloride-the effect on morphology and function of the immune system in mice. Gent. European Journal of Public Health 4:16- 20.

Kaneto, H., Kajimoto, Y., Miyagawa, J., Matuoka, T., Fujitani, Y., Umayahara, Y., Hanafusa, T., Matsuzawa, Y., Yamasaki, Y. and Hori, M. (1999). Beneficial effect of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes. 48(12): 2398-2406.

Mandal, A., Karmakar, R., Bandyopadhyay, S. and Chatterjee, M. (1998). Antihepatotoxic potential of Trianthema portulacastrum in carbon tetrachloride-induced chronic hepatocellular injury: Biochemical characteristics. Archives of Pharmaceutical Research 21:223- 230.

McCord, J. M., Gao, B., Leff, J. and Flores, S. C. (1994). Neutrophil-generated free radicals: Possible mechanisms of injury in adult respiratory distress syndrome. Environment Health Perspective. 102:57- 60.

McKnight,D. C., Mills,R.G., Bray,J. J. and Crag, P. A. (1999). Human Physiology. 4th Edn. Churchill Livingstone, pp. 290.

NIH (1985). National Institute of Health. Guide for the Care and Use of Laboratory Animals. USA, Washington DC: National Research Council, NIH Publication No. 83-127.

Nuhu, A.M., Mshelia.M.S. And Yakubu, Y. (2000). Antimicrobial screening of the bark extract of Pterocarpus erinaceous tree. Journal of Chemical Society of Nigeria. 25:85-92.

Okerulu, I. O. and Ani, C. J. (2001). The phytochemical analysis and antibacterial screening of extracts of Tetracarpidium conophorum. Journal of Chemical Society Nigeria 26(1): 53-55.

Reid, H. I. and Ugwu, A. C. (1987). A simple technique for rapid determination of plasma viscosity. Nigeria Journal of Physiological Science. 3: 45-48.

Yakubu, M. T, Akanji, M. A. and Oladiji, A. T. (2007). Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem. Pharmacognosy Magazine 3:34-38.

Published

2012-12-20

How to Cite

Effect of methanolic extract of Tetracarpidium conophorum (African walnut) seeds on haematological parameters of carbon tetrachloride treated male rats. (2012). Nigerian Journal of Biochemistry and Molecular Biology, 27(1&amp;2), 64-69. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/285