Combined Effect of Sorghum Bicolor Formulated Diet and Glibenclamide on Some Biochemical Changes in Alloxan-Induced Diabetic Rats
Abstract
Food-drug synergy and safety are primarily focused on the potential benefits of using foods to lower the risk of chronic diseases. For the past few years, reports have shown that whole grains, particularly sorghum (Sorghum bicolor), may be potential alternatives to ameliorate type 2 diabetes mellitus (T2DM) symptoms. This study, evaluated the combined effect of sorghum formulated diet and glibenclamide on alloxan induced diabetic rats. Fifty-five (55) albino rats (Wistar strain) were randomly divided into eleven groups of five rats each. The animals received different dosage of the drug (1 mg/kg, 0.5 mg/kg and 0.25 mg/kg), formulated diet (20 g/kg, 15 g/kg and 10 g/kg b.w) and the combination for 28 days. The glycemic index (GI) and glycemic load (GL) of the formulated diet were determined. Fasting blood glucose of rats was measured weekly while lipid profiles were estimated. The GI and GL were found to be 38.02% and 18.39% respectively. There were significant (p<0.05) reductions in the FBG concentrations of the groups treated with diet + glibenclamide compared to the negative control throughout the treatment period with a pronounced at third and fourth week. A significant reduction was observed in LDL-cholesterol (p<0.001), total cholesterol (p<0.05), and triglycerides (p<0.05) levels in groups given a combined treatment as compared to groups given single treatments of diet or glibenclamide. It can be concluded that a combination of the formulated diet and glibenclamide may be useful in the management of diabetes mellitus.
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