Cucumeropsis mannii (African White Melon) Seed Oil Mitigates Dysregulation of Redox Homeostasis, Inflammatory Response, and Apoptosis in Testis of Bisphenol A Exposed Male Rats

Authors

  • Patrick M. Aja Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria. https://orcid.org/0000-0001-7805-9027
  • Peter C. Agu Department of Science Laboratory Technology, Biochemistry Option, Our Savior Polytechnic Enugu, Nigeria. https://orcid.org/0000-0003-4739-2040
  • Hilary A. Ogwoni Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Ugbala E. Ekpono Department of Science Laboratory Technology, Biochemistry Option, Federal Polytechnic Oko, Nigeria.
  • Ernest M. Ezeh Department of Science Laboratory Technology, Biochemistry Option, Our Savior Polytechnic Enugu, Nigeria. https://orcid.org/0000-0002-0164-9325
  • Onyedika G. Ani Department of Clinical Sciences and Nutrition, University of Chester, Parkgate Road Tower Building, United Kingdom https://orcid.org/0000-0002-0986-8020
  • Atamgba A. Asuk Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Cross River University of Technology, Calabar, Nigeria
  • Joshua N. Awoke Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Boniface A. Ale Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria. https://orcid.org/0000-0002-3060-066X
  • Felix E. Nwite Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Oliver U. Ukachi Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria. https://orcid.org/0000-0002-8901-9984
  • Obasi U. Orji Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Peter C. Nweke Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria. https://orcid.org/0000-0002-1174-115X
  • Ugbala E. Ekpono Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Gift O. Ewa Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Ikechuku O. Igwenyi Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Esther U. Alum Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria. https://orcid.org/0000-0003-4105-8615
  • Darlington C. Chukwu Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria. https://orcid.org/0000-0002-1241-605X
  • Ekenechukwu Maduagwuna, Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.
  • Francisca C. Nwiziogo Department of Science Laboratory Technology, Biochemistry Option, Our Savior Polytechnic Enugu, Nigeria.

DOI:

https://doi.org/10.2659/njbmb..74

Keywords:

CMSO, BPA, Testicular toxicity, Redox Homeostasis, Inflammations, Apoptosis

Abstract

Testicular toxicity is a prime threat to male reproductive health. Humans are constantly exposed to Bisphenol A (BPA) via oral route, thus inducing reproductive toxicity. Cucumeropsis mannii seed oil (CMSO) has nutraceuticals with antioxidant potential. This present study investigated the potential of CMSO to mitigate testicular oxidative damages induced by exposure to BPA. Thirty-six (36) male Wistar albino rats, 2-3 months old, were randomly assigned into six (6) study groups; 3 control groups (CG1, CG2, and CG3) and 3 test groups (TG1, TG2, and TG3), respectively. CG1 rats received 1 ml of olive oil. CG2 rats received 100 mg/kg body weight of BPA. CG3 rats received 7.5 ml/Kg body weight of CMSO. TG1 rats were co-administered 100 mg/kg body weight of BPA and 7.5 ml/kg bodyweight of CMSO. TG2 rats were co-administered with 100 mg/kg body weight of BPA and 5 ml/kg body weight of CMSO. TG3 rats were co-administered with 100 mg/kg body weight of BPA and 2.5 ml/kg body weight of CMSO. After the trial, the rats' testes were extracted and taken for biochemical analysis. The result showed that the exposure of rats to BPA significantly (p < 0.05) increased the levels of ROS and MDA but considerably decreased the levels of GSH, CAT, SOD, GPx, IL-1β, IL-6, TNF-α, NF-ĸB, and Caspase-3 activity in the testicular homogenate. However, co-administration of BPA with CMSO significantly (p < 0.05) reversed all the dysregulations. This study reported that CMSO is a potential nutraceutical for mitigation of BPA-induced testicular oxidative damages.

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Published

2022-12-30

How to Cite

Aja, P. M., Agu, P. C., Ogwoni, H. A., Ekpono, U. E., Ezeh, E. M., Ani, O. G., Asuk, . A. A., Awoke, J. N., Ale, B. A., Nwite, F. E., Ukachi, O. U., Orji, O. U., Nweke, P. C., Ekpono, U. E., Ewa, G. O., Igwenyi, I. O., Alum, E. U., Chukwu, D. C., Maduagwuna, , E., & Nwiziogo , F. C. (2022). Cucumeropsis mannii (African White Melon) Seed Oil Mitigates Dysregulation of Redox Homeostasis, Inflammatory Response, and Apoptosis in Testis of Bisphenol A Exposed Male Rats. Nigerian Journal of Biochemistry and Molecular Biology, 37(4), 272–281. https://doi.org/10.2659/njbmb.74