Effects of Aqueous Extracts of Some Citrus Peels on key Enzymes Linked With Type-2 diabetes

Authors

  • Oboh, G. Department of Biochemistry, Federal University of Technology, Akure, Ondo-State, Nigeria Author
  • Ademosun, A. O. Department of Biochemistry, Federal University of Technology, Akure, Ondo-State, Nigeria Author

Abstract

An approach to controlling blood glucose levels in individuals with type-2 diabetes is the inhibition of enzymes involved in the breakdown of starch and absorption of glucose, such as α-amylase and α-glucosidase. This study therefore, sought to characterize the interaction of aqueous extracts from some Nigerian citrus peels [orange (Citrus sinensis), grapefruit (Citrus paradisii) and shaddock (Citrus maxima)] with α-amylase and α-glucosidase in vitro. The total phenolic, flavonoid, vitamin C content and antioxidant activities were also determined. The results revealed that orange peels had the highest total phenol content (8.33mg/g) and flavonoid content (0.58mg/g) as compared to grapefruit [total phenol (6.50mg/g), flavonoid (0.29mg/g)] and shaddock peels [total phenol (5.00mg/g), flavonoid (0.15mg/g)]. Also, orange peels’ aqueous extracts showed the highest antioxidant potential as typified by a higher reducing power, ABTS radical scavenging ability and inhibition of Fe2+ - and sodium nitroprusside- induced lipid peroxidation in rat’s pancreas – in vitro. The aqueous extracts from the citrus peels inhibited α-glucosidase and α-amylase activities, though with a stronger inhibition of α-glucosidase [orange (37.50%), grape (50.0%), shaddock (37.50%)] than α-amylase activities [orange (25.32%), grape (29.29%), shaddock (21.34%)]. The phenolic contents, vitamin C contents, antioxidant activities and inhibition of key enzymes linked with type 2 diabetes suggests the potential use in the control of blood glucose levels associated with type-2 diabetes.

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Author Biography

  • Ademosun, A. O., Department of Biochemistry, Federal University of Technology, Akure, Ondo-State, Nigeria

    An approach to controlling blood glucose levels in individuals with type-2 diabetes is the inhibition of enzymes involved in the breakdown of starch and absorption of glucose, such as α-amylase and α-glucosidase. This study therefore, sought to characterize the interaction of aqueous extracts from some Nigerian citrus peels [orange (Citrus sinensis), grapefruit (Citrus paradisii) and shaddock (Citrus maxima)] with α-amylase and α-glucosidase in vitro. The total phenolic, flavonoid, vitamin C content and antioxidant activities were also determined. The results revealed that orange peels had the highest total phenol content (8.33mg/g) and flavonoid content (0.58mg/g) as compared to grapefruit [total phenol (6.50mg/g), flavonoid (0.29mg/g)] and shaddock peels [total phenol (5.00mg/g), flavonoid (0.15mg/g)]. Also, orange peels’ aqueous extracts showed the highest antioxidant potential as typified by a higher reducing power, ABTS radical scavenging ability and inhibition of Fe2+ - and sodium nitroprusside- induced lipid peroxidation in rat’s pancreas – in vitro. The aqueous extracts from the citrus peels inhibited α-glucosidase and α-amylase activities, though with a stronger inhibition of α-glucosidase [orange (37.50%), grape (50.0%), shaddock (37.50%)] than α-amylase activities [orange (25.32%), grape (29.29%), shaddock (21.34%)]. The phenolic contents, vitamin C contents, antioxidant activities and inhibition of key enzymes linked with type 2 diabetes suggests the potential use in the control of blood glucose levels associated with type-2 diabetes.

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Published

2011-12-30

How to Cite

Effects of Aqueous Extracts of Some Citrus Peels on key Enzymes Linked With Type-2 diabetes. (2011). Nigerian Journal of Biochemistry and Molecular Biology, 26(2), 116-125. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/275