Nutritional and toxicological aspects of Aspergillus niger fermented cassava (Manihot esculenta, Crantz) products-based diets


  • Oboh, G. Biochemistry Department, Federal University of Technology Author


This study sought to evaluate the nutritional and toxicological potential of Aspergillus niger fermented cassava products (flour and gari). Cassava mash was fermented with pure strain of A. niger for 72 days and subsequently processed to flour and gari (the forms in which it is popularly consumed in Nigeria). Three weeks old Wistar rats were assigned into 3 groups: 1-3 such that Group 1 was fed with the basal diet (control) while Groups 2 and 3 were fed with the A. niger fermented gari- and flour-based diets (40 % inclusion) and water ad libitum for 21days. Feed intake, weight gain and feaces were monitored while haematological indices, serum enzymes and metabolites were determined at the end of the feeding experiment. The results revealed that there was no significant change (P>0.05) in the average daily feed intake, daily weight gain, feed conversion ratio, apparent and dry matter digestibility, and nitrogen intake of the rats fed A. niger fermented cassava products diet. However, there was significant increase (P<0.05) in the serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities in rats fed A. niger fermented cassava products; while there was no significant (P>0.05) change in the total bilirubin and albumin in rats fed cassava flour. Furthermore there were no significant (P>0.05) changes in the Packed Cell Volume (PCV) and White Blood Cell (WBC), while there was significant increase (P<0.05) in the Red Blood Cell (RBC). However, the A. niger fermented cassava products (flour and gari) could not be considered safe


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Akindahunsi, A. A., Oboh, G. and Oshodi, A. A. (1999). Effect of fermenting cassava with Rhizopus oryzae on the chemical composition of its flour and gari, Riv Ital Sostanze Grasse. 76: 437-440.

Aletor, V. A. (1993). Cyanide in garri. 2: Assesssment of some aspects of the nutrition, biochemistry and haematology of the rats fed garri containing varying residual cyanide levels. Int J Food Sci Nut. 44: 289 - 292.

American Liver Foundation (1995): Liver function tests,

Aning, K. G., Ologun, A. G., Onifade, A., Alokan, J. A., Adekola, A. I. and Aletor, V. A. (1998). Effects of replacing dried brewer’s grains with sorghum rootlets on growth, nutrient utilization and some blood constituents in the rat. Animal Feed Sci. Tech., 71: 185-190.

Bailey, K. V. (1961). Rural Nutrition Studies in Indonesia III, Epidemiology of hunger Edema in the cassava area, Trop. Geo. Med.,13: 289-295.

Belewu, M. A. and Jimoh, N. O. (2005). Blood, carcass and organ measurements as influenced by Aspergillus’ treated cassava waste in the diet of West African Dwarf (WAD) goat. Global J. Agric. Sci., 4: 125-128.

Bressani, R. (1999). Nutritional evaluation in humans. Bioconversion of organic residues for rural communities.

Canope, J. and Tamiya, B. (1977). Preliminary experiments in the use of chlorella as human food. Food Technol. J., 8: 179-182.

Cock, H. J. (1985). Cassava new potential for a neglected crop. Colorado, Boulder: Westview press.

Collins, J. L. and Falasinnu, G. A. (1977). Yam (Dioscorea spp) flour fortification with soy flour. J. Food Sci., 42, 821-833.

Conn, E. E. (1973). Cyanogenic glucosides: their occurrence biosynthesis and function. In chronic cassava toxicity. Nestle, B. and Mclntyer, B. (eds). Canada, Ottawa: International Development Research Centre. p. 55-63.

David, E. and Johnston, M. D. (1999). Special considerations in interpreting liver function tests. American Family Physician. Published by the American Academy of Family Physicians.

Dhilon, J. K. and Shirvaram, N. (1999). Biodegradation of cyanide compounds by a Pseudomonas species. Can. J. Microb., 45: 201-208

Ejukonemu, F. E. and Nwafor, O. E. (2004). Bioconversion of cassava wastes for protein enrichment using amylolytic fungi. A preliminary report. Global J. Pure & App. Sci., 10:505-507.

Oboh, G. (2006). Nutrient enrichment of cassava peels using Saccharomyces cerevisae & Lactobacillus spp solid media fermentation techniques. Elect. J. Biotech., 9: 46-49.

Oboh, G. and Akindahunsi, A. A. (2003). Biochemical changes in cassava products (flour & gari) subjected to Saccharomyces cerevisae solid media fermentation. Food Chem., 82: 599-602.

Oboh, G. and Akindahunsi, A. A. (2005). Nutritional and toxicological evaluation of Saccharomyces cerevisae fermented cassava flour. J. Food Comp. Anal., 18: 731 – 738.

Oboh, G. and Akindahunsi, A. A. and Oshodi A. A. (2000). Aflatoxin and moisture content of micro-fungi fermented cassava products (flour and gari). App. Trop. Agric., 5: 154-157.

Oboh, G., Akindahunsi, A. A. and Oshodi, A. A. (2002). Nutrient and anti-nutrient content of Aspergillus niger fermented cassava products (flour and gari). J Food Comp. Anal., 15: 617-622.

Oboh, G., Akindahunsi, A. A. and Oshodi, A. A. (2003). Dynamics of Phytate-Zn balance of fungi fermented cassava products (Flour & Gari). Plant Food Hum. Nut., 58: 1-7.

Oboh, G. and Oladunmoye, M. K. (2007). Biochemical changes in Micro-Fungi fermented Cassava Flour produced from Low- And Medium-Cyanide variety of Cassava Tubers. Nut. Health., 18: 355 - 367

O’Brien, G. M., Taylor, A. J. and Poulter, N. H. (1991). Improved enzymatic assay for cyanogens in fresh and processed cassava J. Sci. Food Agric., 56: 277-289.

Odetokun, S. M., Aiyesanmi, F. A. and Esuoso, K. O. (1998). Enhancement of nutritive value of pupuru, a fermented cassava

products. Riv Ital Sostanze Grasse, 75: 155-158.

Oshodi, A. A. (1988). Protein enrichment of foods that are protein deficient. II. Fortification of processed cassava (Gari) with bovine blood plasma protein concentrate. Nig. J. App. Sci., 6: 61- 64.

Reade, A. E. and Gregory, K. F. (1975). High temperature protein Enriched feed from cassava fungi. App. Microb., 30: 897-907.

Reinbault, M. and Alazard, D. (1980). Culture method to study fungal growth in solid fermentation. Eur. J. App. Microb. Biotech., 9: 199-209.

Van Weerden, E. J. (1999). Nutritional evaluation of bioconversion products for farm animal.

Zar, J. H. (1984). Biostatistical Analysis. USA: Prentice-Hall, Inc.. p. 620



How to Cite

Nutritional and toxicological aspects of Aspergillus niger fermented cassava (Manihot esculenta, Crantz) products-based diets. (2011). Nigerian Journal of Biochemistry and Molecular Biology, 26(1), 1-9.