Phytochemical Composition and Radical Scavenging Activity of Hexane, Ethyl acetate and Methanol Extracts of Spermacoce ocymoides (Burm F.) DC and Annona muricata L. Leaves and Fruit Juice
Abstract
This study investigated the in vitro free radical scavenging capacity and phytochemical constituents of methanol, ethyl
acetate and n-hexane extracts of leaves of Spermacoce ocymoides (Burm F.) DC (Rubiaceae) and Annona muricata L.
(Annonaceae) as well as the fruit juice of A. muricata. A. muricata fruit juice was also investigated for its vitamin composition.
The radical scavenging activities of the solvent extracts of the leaves and fruit juice were determined by measuring their reducing
activities against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, hydrogen peroxide and ferric ion. The methanol, ethyl acetate
and hexane extracts of S. ocymoides and A. muricata leaves contained phenolics, alkaloids and tannins, while only the methanol
and ethyl acetate extracts of both plants contained flavonoids and triterpenes, respectively. Saponins were present in the hexane
extracts of both plants and ethyl acetate extract of A. muricata leaves. Steroids, phlobatannins and glycosides were not detected in
the extracts. A. muricata fruit juice contained the highest amount of flavonoids while the A muricata methanol leaf extract
contained the least amount of flavonoids. S. ocymoides methanol leaf extract contained the highest amount of phenolics, while
the A muricata ethyl acetate leaf extract contained the least amount of phenolics. A muricata fruit juice contained more vitamin C
than vitamin B1, with vitamin B2 having the lowest concentration. The extracts of the leaves of both plants as well as A. muricata
fruit juice reduced DPPH, hydrogen peroxide and ferric ion at concentrations varying from 0.2 mg/ml to 1.0 mg/ml.
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Ahalya, B., Ravishankar, K. and PriyaBandhavi, P. (2013). Evaluation of in vitro anti oxidant activity of Annona muricata bark. International Journal of Pharmaceutical, Chemical and Biological Sciences 3(2): 406-410
Ayyanar, M. and Ignacimuthu, S. (2009). Herbal medicines for wound healing among tribal people in Southern India: Ethnobotanical and scientific evidences. International Journal of Applied Research in Natural Products 2(3): 29-42.
Baskar, R., Rajeswari, V. and Kumar, T. S. (2007). In vitro antioxidant studies in leaves of Annona species. Indian Journal of Experimental Biology 45: 480-485.
Bender, D. A. and Mayes, P. A. (2003). Vitamins & Minerals. In: Harper’s Illustrated Biochemistry, Twenty-sixth edition (Murray, R. K., Granner, D. K., Mayes, P. A. and Rodwell, V. W. Eds), The McGraw-Hill Companies Inc., New York, pp 481-497.
Boakye, A. A., Wireko-Manu, F. D., Agbenorhevi, J. K. and Oduro, I. (2015). Antioxidant activity, total phenols and phytochemical constituents of four underutilised tropical fruits. International Food Research Journal 22(1): 262-268.
Burkill, H. M. (1985). Useful Plants of West Tropical Africa. Vol. 1
Conserva, L. M. and Ferreira Jr., J. C. (2012). Borreria and Spermacoce species (Rubiaceae): A review of their ethnomedicinal properties, chemical constituents, and biological activities. Pharmacognosy Reviews 6(11): 46–55.
Dai, Y., Hogan S., Schmelz, E. M., Ju, Y. H., Canning, C. and Zhou, K. (2011). Selective growth inhibition of human breast cancer cells by graviola fruit extract in vitro and in vivo involving down-regulation of EGFR expression. Nutrition and Cancer 63(5): 795–801.
Ghasemzadeh, A., Jaafar, H. Z. E. and Rahmat, A. (2010). Antioxidant activities, total phenolics and flavonoid content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules 15: 4324-4333.
Harborne, J. B. (1973). Phytochemical Methods: A guide to modern techniques of plant analysis. New York: Chapman and Hall.
Harisaranraj, R., Suresh, K. and Saravanababu, S. (2009). Evaluation of the chemical composition Rauwolfia serpentina and Ephedra vulgaris. Advances in Biological Research 3(5-6): 174-178.
Jagadish, L. K., Krishnan, V. V., Shenbhagaraman, R. and Kaviyarasan, V. (2009). Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricus bisporus imbach before and after boiling. African Journal of Biotechnology 8: 654-661.
Jeyachandran, M., Kumar, T. A. and Gandhimathi, S. (2013). Phytochemical investigation of ethnomedicinal Spermacoce ocymoides roots. Journal of Pharmacognosy and Phytochemistry 2 (3): 86-88.
Moodely, R., Chenia, H., Jonnalagadda, S. B. and Koorbanally, N. (2011). Antibacterial and antiadhesion activity of the pentacyclic triterpenoids isolated from the leaves and edible fruits of Carissa macrocarpa. Journal of Medicinal Plants Research 5: 4851-4858.
Morton, J. F. (1987). "Soursop (Annona muricata)". Fruits of warm climates. Miami: Florida Flair Books. Purdue University, pp. 75–80. Retrieved 28 October 2012.
Muthu, S. and Durairaj, B. (2015). Evaluation of antioxidant and free radical scavenging activity of Annona muricata. European Journal of Experimental Biology 5(3): 39-45.
Nafiu, M. O., Akanji, M. A. and Yakubu, M. T. (2011). Phytochemical and mineral constituents of Cochlospermum planchonii (Hook. Ef. x Planch) root. Bioresearch Bulletin 5:51-58.
Odebiyi, O. O. and Sofowora, E. A. (1978). Phytochemical screening of Nigerian medicinal plants II. Lloydia 41: 234-246.
Okigbo, R. N., Anuagasi, C. L. and Amadi, J. E. (2009). Advances in selected medicinal and aromatic plants indigenous to Africa. Journal of Medicinal Plants Research 3: 86-95.
Okwu, D. E. and Okwu, M. E. (2004). Chemical composition of Spondias mombin Linn plant part. Journal of Sustainable Agriculture and the Environment 6(2): 140-147.
Olajire, A. A. and Azeez, L. (2011). Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African Journal of Food Science and Technology 2: 22-29.
Oloyede, O. B., Ajiboye, T.O., Komolafe, Y. O. and
Salau, A. K. (2013). Polyphenolic extract of Blighia sapida aril prevents N-nitrosodiethylamine-mediated oxidative onslaught on microsomal protein, lipid and DNA. Food Bioscience 1: 48-56.
Oloyede, O. B., Salau, A. K., Akeusola, R. T., Ganiyu, O. T., Azeez, L. and Ogunbode, S. M. (2012). Phytochemical content, radical scavenging and antibacterial properties of aqueous extract of Jatropha curcas Linn leaves. Fountain Journal of Natural and Applied Sciences 1(1): 41–48.
Omaye, S. T., Turbull, T. P. and Sauberchich, H. C. (1979). Selected methods for determination of ascorbic acid in cells, tissues, and fluids. Methods in Enzymology 6: 3–11.
Onawumi, O. O. E., Adelowo, F. E., Ipadeola, A. O., Edewor, T. A. I., Ayoola, P. B. and Odunola, O. A. (2012). Preliminary studies on phytochemical and antimicrobial investigation of plants (Irawo-Ile) Mitracarpus villosus, Euphorbia hirta and Spermacoce ocymoides. International Journal of Research and Reviews in Applied Sciences 10(1): 78-81.
Oskoueian, E., Abdullah, N., Ahmad, S., Saad, W. Z., Omar, A. R. and Ho, Y. W. (2011). Bioactive compounds and biological activities of Jatropha curcas L. kernel meal extract. International Journal of Molecular Sciences 12: 5955-5970.
Oyaizu, M. (1986). Studies on products of browning reaction: antioxidative activities of product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 44: 307-315.
Raybaudi-Massilia, R., Suárez, A. I., Arvelo, F., Sojo, F., Mosqueda-Melgar, J., Zambrano, A. and Calderón-Gabaldón, M. I. (2015). An analysis in-vitro of the cytotoxic, antioxidant and antimicrobial activity of aqueous and alcoholic extracts of Annona muricta L. seed and pulp. British Journal of Applied Science & Technology 5(4): 333-341.
Ruch, R. J., Cheng, S. J., Klaunig, J. E. (1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10:1003-1008.
Salau, A. K, Yakubu, M. T. and Oladiji, A. T. (2015). In vitro and in vivo antioxidant activity of aqueous extracts of Anogeissus leiocarpus (DC) Guill & Perr and Terminalia avicennioides Guill & Perr root barks. Cameroon Journal of Biological and Biochemical Sciences 23: 9-16.
Shahjahan, M., Sabitha, K. E., Mallika, J. and Shyamala-Devi, C. S. (2004). Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian Journal of Medical Research 120: 194-198.
Shahjahan, M., Vani, G. and Shyamaladevi, C. S. (2005). Effect of Solanum trilobatum on the antioxidant status during diethyl nitrosamine induced and phenobarbital promoted hepatocarcinogenesis in rat. Chemico-Biological Interactions 156: 113-123.
Shanmugam, S., Annadurai, M. and Rajendran, K. (2011). Ethnomedicinal plants used to cure diarrhoea and dysentery in Pachalur Hills of Dindigul District in Tamil Nadu, Southern India. Journal of Applied Pharmaceutical Science 1(8): 94-97.
Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I. and Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry 101: 267-273.
Upadhyay, R. K. (2011). Plant natural products: their pharmaceutical potential against disease and drug resistant microbial pathogens. Journal of Pharmacy Research 4(4): 1179-1185.
Urquiaga, I. and Leighton, F. (2000). Plant polyphenol, antioxidants and oxidative stress. Biological Research 33: 159-165.
Vijayameena, C., Subhashini, G., Loganayagi, M. and Ramesh, B. (2013). Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. International Journal of Current Microbiology and Applied Sciences 2(1): 1- 8.
Yücekutlu, A. N. and Bildacı, I. (2008). Determination of plant saponins and some of Gypsophila species: a review of the literature. Hacettepe Journal of Biology & Chemistry 36(2): 129-135.
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