Effects of Aqueous Extract of Blighia sapida Leaves on Alloxan-induced Diabetic Rats


  • Oladiji, A. T. Department of Biochemistry, University of Ilorin, Ilorin, Nigeria Author
  • Yakubu, M. T. Department of Biochemistry, University of Ilorin, Ilorin, Nigeria Author
  • Odeyemi, S. W. Department of Biochemistry, University of Ilorin, Ilorin, Nigeria Author
  • Oyegoke, R. A. Department of Biochemistry, University of Ilorin, Ilorin, Nigeria Author


Aqueous extract of Blighia sapida leaves was investigated for anti-diabetic activity in alloxan-induced diabetic albino rats. Thirty six albino rats (186.50 ± 7.58 g) were completely randomized into six groups (A-F) such that animals in group A (non-diabetic rats) received orally 0.5 ml of distilled water while the diabetic animals (made diabetic by intraperitoneal administration of single dose of 150 mg/kg body weight) in groups B, C, D, E and F were treated with 0.5 ml of distilled water, 2.5 mg/kg body weight of glibenclamide, 25, 50 and 100 mg/kg body weight of the extract respectively. The levels of blood glucose, serum total cholesterol, triacylglycerol, albumin, creatinine, urea, malondialdehyde, and glycogen in the liver of the animals as well as haemoglobin (Hb) and packed cell volume (PCV) were evaluated. The results revealed that the levels of blood glucose, serum cholesterol, albumin, creatinine, urea, triacylglyceride and malondialdehyde increased significantly (P<0.0.05) in the diabetic animals whereas the liver glycogen, Hb, PCV, liver-, kidney- and pancreas-body weight ratios decreased significantly (P<0.05). These alterations were not significantly different in the diabetic rats administered 25, 50 and 100 mg/kg body weight of the extract whereas these parameters in the diabetic animals treated with glibenclamide compared well with the non-diabetic distilled water administered animal. Contrary to ethno-medicinal claim, this study has revealed that the aqueous extract of Blighia sapida leaves at the doses of 25, 50, 100 mg/kg body weight did not have anti-hyperglycemic activity and is not suitable for managing complications associated with diabetes.


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Abolaji, O. A., Adebayo, A. H. and Odesanmi, O. S. (2007). Nutritional qualities of three medicinal plant parts Xylopia aethiopica, Blighia sapida and Parinari polyandra commonly used by pregnant women in the western part of Nigeria. Pakistan Journal of Nutrition 6: 665-668.

Adeneye A. A., Adeyemi O. O., Agbaje E.O. and Sofidiya M. O. (2012). The novel antihyperglycaemic action of Hunteria umbellata seed fractions mediated via intestinal glucose uptake inhibition. African Journal of Traditional Complementary and Alternative Medicine 9(1):17‐24.

Adisa, R. A., Choudhary, M. I., Adewoye, E. O. and Olorunsogo, O. O. (2010). Hypoglycaemic and biochemical properties of Cnestis ferruginea. African Journal of Traditional Complementary and Alternative Medicine 7 (3):185 – 194.

Antwi, S., Martey, O. N. K., Donkor, K. and Okine, L. K. N. A. (2009). Anti-diarrhoeal activity of Blighia sapida (Sapindaceae) in rats and mice. Journal of Pharmacology and Toxicology 4: 117-125.

Atolani, O., Olatunji, G. A. and Fabiyi, O. A. (2009). Blighia Sapida; The plants and its Hypoglycinsan- Overview. Journal of Scientific Research 39(2):15-25

Blass, K. G., Thierbert, R. J. and Lam, L. K. (1974). A study of the mechanism of the Jaffe reaction. Journal of Clinical Chemistry and Clinical Biochemistry, 12, 336-343.

Bopanna, K. N., Kannan, J., Sushma, G., Balaraman, R. and Ratho, S. P. (1997). Antidiabetic and antihyperlipidaemic effects of neem seed kernel power on alloxan diabetic rabbits. Indian Journal of Pharmacology 29: 162-167.

Buege, J. A. and Aust, S. D. (1978). Microsomal lipid peroxidation. Methods in Enzymology 52: 302-310.

Doumas, B. T., Watson, W. A. and Biggs, H. G. (1971). Albumin standards and the measurement of serum albumin with bromcresol green. Clinical Chemistry Acta 31: 87-96.

Ezeigbo, I. I. and Asuzu, I. U. (2012). Anti-diabetic activities of the methanol leaf extracts of Hymenocardia acida (Tul.) in alloxan-induced diabetic rats. African Journal of Traditional Complementary and Alternative Medicine 9(2):204-209.

Felig, P. E., Ohman, J. L. and Chall, G. F. (1970). Plasma amino acid levels in diabetic ketoacidosis. Diabetes 19: 727-734.

Fossati, P. and Precipe, L. (1982). Serum triglycerides determined colourimetrically with an Enzyme that produces hydrogen peroxide. Clinical Chemistry 28(10): 2077-2080.

Fredrickson, D. S., Levy, R. I. and Lees, R. S. (1967). Fat transport in lipoproteins-an integrated approach to mechanisms and disorders. New England Journal of Medicine 276:215-225.

Gbolade, A. A. (2009). Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. Journal of Ethnopharmacology 121, 135-139.

Goodman, L. S. and Gilman, A. (1985). The pharmacological basis of therapeutics, 7th Edition. Mac Millan, New York. pp. 1490-510.

Gray, A. A. and Flat, P. R. (1999). Insulin releasing like activity of the traditional Antidiabetic plant Coriander sativum (coriander). Journal of Nutrition 81: 203-208.

Horie, S., Ishii, H. and Suga, T. (1981). Changes in peroxisomal fatty acid oxidation in diabetic rat liver. Journal of Biochemistry 90: 1691-1696.

Irvine, F. R. (1965). Botany and Medicine in West Africa. Ibadan University Press, Ibadan Nigeria. pp. 102-130.

Kazeem, M. I., Raimi, O. G., Balogun, R. M. and Ogundajo, A. L. (2013). Comparative Study on the α-Amylase and α-Glucosidase Inhibitory Potential of Different Extracts of Blighia Sapida Koenig. American Journal of Research Communication (in press).

Kean, E. A. and Hare, E. R. (1980). Gamma glutamyl trans-peptidase of the ackee plant Blighia sapida. Phytochemistry 19:199-204.

Kemp, A., Adrienne, J. M. and Heijningen, K. V. (1954) A colorimetric micro-method for the determination of glycogen in tissues. Biochemistry Journal 56(4): 646-648.

Kikkawa, R., Koya, D. and Haneda, M. (2003). Progress of diabetic neuropathy American Journal of Kidney Disfunction 41: 19-21.

Koenig, K. O. (2002). Germplasm resource information network. United States Department of Agriculturre. Retrieved 2011-10-18.

Kristen, L. (2003). Tropical flowering plants: A Guide to identification and cultivation. Timber Press.

Kumarappan, C. T., Rao, T. N and Mandal, S. C. (2007). Polyphenolic extract of Ichnocarpus frutescens modifies hyperlipidemia status in diabetic rats. Journal of Cell and Molecular Biology 6(2): 175–187.

Mbanya, J. C., Bonicci, F. and Nagan, K. (1996). Guidelines for the management of NIDDM in Africa. A consensus document, Greece, Novo Nordisk A/s. pp. 1-35.

Morton, J. F. (1987). Ackee: Fruits of warm climates. In: Morton, J. F. and Miami, F. L. (eds). pp. 269 -271.

Musbayane, C. T., Bwititi, P. T. and Ojewole, J. A. O. (2006). Effect of oral administration of some herbal extracts on food consumption and blood glucose levels in normal and streptozotocin treated diabetic rats. Methods and Findings in Experimental and Clinical Pharmacology 28(4): 223-228.

Odetola, A. A, Akinloye, O., Egunjobi, C., Adekunle, W. A. and Ayoola, A. O. (2006). Possible antidiabetic and antihyperlipidemic effect of fermented Parkia Biglobosa (JACQ) extract in alloxan-induced diabetic rats. Clinical and Experimental Pharmacology and Physiology 33: 808-812.

Oladiji, A. T., Shoremekun, K. L. and Yakubu, M. T. (2009). Physicochemical properties of the oil from the fruit of Blighia sapida and toxicological evaluation of the oil-based diet in Wistar rats. Journal of Medicinal Food 12(5): 1127-1135.

Oyedemi, S. O., Yakubu, M. T. and Afolayan A. J. (2011). Antidiabetic activities of aqueous leaves extract of Leonotis leonurus in streptozotocin-induced diabetic rats. Journal of Medicinal Plants Research 5(1):119-125.

Pari, L. and Amarnath, S. (2004). Antidiabetic activity of Boerhavia diffusa L. effect on hepatic key enzymes in experimental diabetes. Journal of Ethnopharmacology 91: 109-113.

Pari, L. and Latha, M. (2002). Effect of Cassia auriculata flowers on blood sugar levels, serum and tissue lipids in streptozotocin diabetic rats. Singapore Medical Journal 43(12): 617-621.

Quattara, H., Niamké, B., Dally, T. and Kati-Coulibaly, S. (2010). Nutritional composition studies of sun dried Blighia sapida (K. koenig) aril from Côte d’Ivoire, Journal of Applied Bioscience 32: 1989-1994.

Robert, K. M., Daryl, K. G., Peter, A. M. and Victor, W. R. (2003). Harper’s Illustrated Biochemistry, a LANGE Medical Book. 26th edition.

Robert, R. (1998). The Tropical Look. Timber Press.

Rodríguez De La P., Gloria C., Hleap, Z., José I., Zuluaga, G. and Claudia, L. (2012). Evaluación Del Extracto Del Arilo Del Ackee (Blighia Sapida K) Con Propiedades Hipoglucemiantes, En Biomodelos Biotecnología en el Sector Agropecuario y Agroindustrial, 10(2): 8.

Saidu, A. N., Mann, A. and Onuegbu, C. D. (2012). Phytochemical screening and hypoglycaemic effect of aqueous Blighia sapida root bark extract on normoglycemic albino rats. British Journal of Pharmaceutical Research 2(2): 89-97.

Schwartz, S. L. (2006). Diabetes and dyslipidemias. Diabetes Obesity Metabolism 8: 355-364.

Sokeng, D. S., Rokeya, B., Mostafa, M., Nahar, N., Mosihuzzaman, N., Ali, L. and Kamtchouing, P. (2005). Antihyperglycemic effect of Bridellia ndellernsis ethanol extract and fractions in streptozotocin-induced diabetic rats. African Journal of Traditional Complementary and Alternative Medicine 2:94-102.

Szkudelski, T. (2001). The mechanism of alloxan and streptozotocin action-cells of the rat pancreas. Physiology Research 50: 536-546

Trivedi, N. A., Mazumder, B., Bhatt, J. D. and Hemavathi, K. G. (2004). Effect of Shilajit on blood glucose and lipid profile in alloxan-induced diabetic rats. Indian Journal of Pharmacology 36: 373-376.

Udoh, A. E., Iya, N., Okon, E. and Mary, N. (2007). Red cell catalase activity in diabetics. Pakistan Journal of Nutrition 6: 511-515.

Veniamin, M. P. and Varkirtzi, C. (1970). Chemical basis of the carbamidodi-acetyl micro-method for estimation of urea, citruline and carbamyl derivatives Clinical Chemistry 16: 3-6

Wadkar, R. R., Supale, R. S., Tiwari, K. M., Patil, K. S. and Jalalpuri, S. S. (2008). Screening of roots of Baliospermum montanum for hepatoprotective activity against paracetamol induced liver damage in albino rats. International Journal of Green Pharmacy 220- 223.

WHO (1999). WHO news: Traditional medicine strategy launched. Bullettin of the World health Organization 80: 610-610.

Wild, S., Roglic, G., Green, A., Sicree, R. and King, H. (2004). Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 27: 1047-1053.

Yadav, J. P., Kalia, A. N. and Dangi, A. S. (2008). Hypoglycemic activity of the extract of Salvadora oleoides in normal and alloxan induced diabetes rats. Indian Journal of Pharmacology 40:23-27.

Yakubu, M. T. (2012). Effect of a 60-day oral gavage of a crude alkaloid extracts from Chromolaena odoratum leaves on hormonal and spermatogenic indices of male rats. Journal of Andrology 33(6): 1199-1207.

Yakubu, M. T., Akanji, M. A. and Oladiji, A. T. (2008). Effect of oral administration of aqueous extract of Fadogia agrestis stem on some testicular function indices of male rats. Journal of Ethnopharmacology 111(2): 288–292.

Yakubu, M. T., Awotunde, S. O., Ajiboye, T. O., Oladiji, A. T. and Akanji, M. A. (2011). Pro-sexual effects of aqueous extracts of Massularia acuminata root in male Wistar rats. Andrologia 43 (5): 334-340.

Yakubu, M. T., Salau, A. K., Oloyede, O. B. and Akanji, M. A. (2013). Anti-diabetic activity of aqueous leaf extract of Ficus exasperata in

alloxan-induced diabetic Wistar rats. Cameroon Journal of Experimental Biology (in press)



How to Cite

Effects of Aqueous Extract of Blighia sapida Leaves on Alloxan-induced Diabetic Rats. (2013). Nigerian Journal of Biochemistry and Molecular Biology, 28(1&amp;2), 11-21. https://www.nsbmb.org.ng/journals/index.php/njbmb/article/view/311