Antioxidant and Protective Activities of Aqueous Theobroma cacao Seed Extract against Aluminium-induced Hippocampal Toxicity in Wistar Rats
Keywords:
Aluminium, Neurotoxicity, Neuroprotection, Hippocampus, Theobroma cacaoAbstract
Aluminium (Al) is a known toxic metal linked to oxidative damage and neurodegeneration. Hence, the antioxidant and neuroprotective effects of aqueous Theobroma cacao seed extract (CO) in aluminium chloride-exposed Wistar rats was examined. The study consisted of six (6) groups containing six (6) Wistar rats each. Group A (Control) received 1 ml of distilled water/day. Group B (Al) received 100 mg/kg body weight (BW) of Al only. Group C received 250 mg/kg BW/day of CO and 100 mg/kg BW of Al. Group D received 500 mg/kg BW of CO and 100 mg/kg BW of Al. Group E received 250 mg/kg BW of CO only. Group F received 500 mg/kg BW of CO only. The administration was via an orogastric tube for 28 days after which the Novel Object Recognition (NOR) neurobehavioral test was conducted. Thereafter, the rats were sacrificed and the hippocampi were harvested for antioxidant and histological evaluations. A significant decrease in the antioxidant enzymes activities, final body weight and neurobehavioural functions were observed in the rats treated with Al only following comparison with the control and CO-treated groups. However, CO protected against these Al-induced effects. Also, several histological alterations were observed in the Al groups, whereas control and CO-treated groups were relatively normal. Taken together, CO had potent antioxidant and protective activities against Al-induced toxicity in the experimental rats.
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Anand, K. S. and Dhikav, V. (2012). Hippocampus in health and disease: An overview. Annals of Indian Academy of Neurology, 15(4): 239.
Andújar, I., Recio, M. C., Giner, R. M. and Ríos, J. (2012). Cocoa polyphenols and their potential benefits for human health. Oxidative Medicine and Cellular Longevity, 2012:23.
Azzaoui, F. Z., Ahami, A. O. and Khadmaoui, A. (2008). Impact of aluminum sub-chronic toxicity on body weight and recognition memory of wistar rat. Pakistan Journal of Biological Sciences, 11(14): 1830-1834.
Baharum, Z., Akim, A. M., Hin, T. Y. Y., Hamid, R. A. and Kasran, R. (2016). Theobroma cacao: review of the extraction, isolation, and bioassay of its potential anti-cancer compounds. Tropical Life Sciences Research, 27(1): 21.
Bittencourt, L. O., Damasceno-Silva, R. D., Aragão, W. A. B., Eiró-Quirino, L., Oliveira, A. C. A., Fernandes, R. M. and Lima, R. R. (2022). Global proteomic profile of aluminum-induced hippocampal impairments in rats: are low doses of aluminum really safe?. International Journal of Molecular Sciences, 23(20): 12523.
Buege, J. A. (1978). Microsomal lipid peroxidation. Methods in Enzymology, 52: 302-310.
Cohen, G., Dembiec, D. and Marcus, J. (1970). Measurement of catalase activity in tissue extracts. Analytical Biochemistry, 34(1): 30-38.
Dey, M. and Singh, R. K. (2022). Neurotoxic effects of aluminium exposure as a potential risk factor for Alzheimer’s disease. Pharmacological Reports, 74(3): 439-450.
Drury, R. A. B. and Wallington, E. A. (1980). Carleton’s histological technique 5th ed. New York: Churchill Livingstone, Oxford University Press: pp520
Enogieru, A. B. and Egbon, F. O. (2022). Neurobehavioural and histological alterations in lead acetate-exposed rats pretreated with aqueous leaf extract of Vernonia amygdalina. African Journal of Biomedical Research, 25(2): 205-213.
Enogieru, A. B. and Inegbedion, G. O. (2022). Attenuation of oxidative stress and cognitive impairment in cadmium chloride-exposed wistar rats pre-treated with ethanolic turmeric root extract. The Journal of Phytopharmacology, 11(2): 118-124.
Enogieru, A. B. and Momodu, O. I. (2021). The developing cerebellum as a target for toxic substances: protective role of antioxidants. The Cerebellum, 20:1-17.
Enogieru, A. B. and Omoruyi, S. I. (2022). Exploration of aqueous Phyllanthus amarus leaf extract as a protective agent in mercury chloride-exposed wistar rats: A neurobehavioural study. Journal of Applied Sciences and Environmental Management, 26(4): 629-637.
Enogieru, A. B., Omoruyi, S. I. and Ekpo, O. E. (2018). Antioxidant and apoptosis-inhibition potential of Carpobrotus edulis in a model of parkinson’s disease. Journal of African Association of Physiological Sciences, 6(2): 126-135.
Evans, W. C. (2009). Trease and Evans' pharmacognosy. Elsevier Health Sciences: pp 47-135
Exley, C. (2004). The pro-oxidant activity of aluminum. Free Radical Biology and Medicine, 36(3): 380-387.
Exley, C. (2013). Human exposure to aluminium. Environmental Science: Processes and Impacts, 15(10): 1807-1816.
Fernández‐Millán, E., Cordero‐Herrera, I., Ramos, S., Escrivá, F., Alvarez, C., Goya, L., and Martín, M. A. (2015). Cocoa‐rich diet attenuates beta cell mass loss and function in young Zucker diabetic fatty rats by preventing oxidative stress and beta cell apoptosis. Molecular Nutrition and Food Research, 59(4): 820-824.
Gemmell, E., Bosomworth, H., Allan, L., Hall, R., Khundakar, A., Oakley, A. E. and Kalaria, R. N. (2012). Hippocampal neuronal atrophy and cognitive function in delayed poststroke and aging-related dementias. Stroke, 43(3): 808-814.
Ighodaro, O. M. and Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4): 287-293.
Ishaq, S. and Jafri, L. (2017). Biomedical importance of cocoa (Theobroma cacao): significance and potential for the maintenance of human health. Matrix Science Pharma, 1(1): 1-5.
Julka, D., Vasishta, R. K. and Gill, K. D. (1996). Distribution of aluminum in different brain regions and body organs of rat. Biological Trace Element in Research, 52: 181-192.
Kandimalla, R., Vallamkondu, J., Corgiat, E. B. and Gill, K. D. (2016). Understanding aspects of aluminum exposure in alzheimer's disease development. Brain Pathology, 26(2): 139-154.
Kebe, E. O., Oria, R. S., Ugwuja, J. O. and Enya, E. L. (2019). Neurotoxicity of aqueous extract of Theobroma cacao on the cerebellum of adult wistar rats. Acta Scientific Neurology, 2: 72-75.
Kim, H. J., Kong, M. K. and Kim, Y. C. (2008). Beneficial effects of Phellodendri Cortex extract on hyperglycemia and diabetic nephropathy in streptozotocin-induced diabetic rats. BMB Reports, 41(10): 710-715.
Kim, J., Lee, K. W. and Lee, H. J. (2011). Cocoa (Theobroma cacao) seeds and phytochemicals in human health. In Nuts and seeds in health and disease prevention, Academic Press: pp. 351-360.
Lamuela-Raventós, R. M., Romero-Pérez, A. I., Andrés-Lacueva, C. and Tornero, A. (2005). Health effects of cocoa flavonoids. Food Science and Technology International, 11(3): 159-176.
Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54: 275-287.
Malik, J., Kumar, M., Deshmukh, R. and Kumar, P. (2013). Ameliorating effect of lyophilized extract of Butea frondosa leaves on scopolamine-induced amnesia in rats. Pharmaceutical Biology, 51(2): 233-239.
Marosi, K., Bori, Z., Hart, N., Sárga, L., Koltai, E., Radák, Z. and Nyakas, C. (2012). Long-term exercise treatment reduces oxidative stress in the hippocampus of aging rats. Neuroscience, 226: 21-28.
Martín, M. A., Goya, L. and de Pascual-Teresa, S. (2020). Effect of cocoa and cocoa products on cognitive performance in young adults. Nutrients, 12(12): 3691.
Mastroiacovo, D., Kwik-Uribe, C., Grassi, D., Necozione, S., Raffaele, A., Pistacchio, L. and Desideri, G. (2015). Cocoa flavanol consumption improves cognitive function, blood pressure control, and metabolic profile in elderly subjects: the cocoa, cognition, and aging (CoCoA) study—a randomized controlled trial. The American Journal of Clinical Nutrition, 101(3): 538-548.
Maya, S., Prakash, T., Madhu, K. D. and Goli, D. (2016). Multifaceted effects of aluminium in neurodegenerative diseases: A review. Biomedicine and Pharmacotherapy, 83: 746-754.
Misra, H. P. and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 247(10): 3170-3175.
Mitkus, R. J., King, D. B., Hess, M. A., Forshee, R. A. and Walderhaug, M. O. (2011). Updated aluminum pharmacokinetics following infant exposures through diet and vaccination. Vaccine, 29(51): 9538-9543.
Montilla, P., Barcos, M., Munoz, M. C., Bujalance, I., Munoz-Castaneda, J. R. and Tunez, I. (2005). Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats. Journal of Biochemistry and Molecular Biology, 38(5): 539-544.
Nayak, P. and Chatterjee, A. K. (2001). Effects of aluminium exposure on brain glutamate and GABA systems: an experimental study in rats. Food and Chemical Toxicology, 39(12): 1285-1289.
Nyman, M. (1959). Serum hatoglobin; methodological and clinical studies. Scandinavian Journal of Clinical and Laboratory Investigation, 11: 1-169.
Ogueche, P. N., Ugwu, C. E., Ezejindu, D. N., Omeje, M., Dike, C. C., Okonkwo, C. O. and Obidoa, H. C. (2014). Aluminum intoxication induced biochemical and histopathological alterations in male wistar albino rats hepotocytes. Journal of Natural Sciences Research, 4(24): 2224-3186.
Parkinson, I. S., Ward, M. K. and Kerr, D. N. J. (1981). Dialysis encephalopathy, bone disease and anaemia: the aluminum intoxication syndrome during regular haemodialysis. Journal of Clinical Pathology, 34(11): 1285.
Pitsikas, N., Rigamonti, A. E., Cella, S. G., Locatelli, V., Sala, M. and Muller, E. E. (2001). Effects of molsidomine on scopolamine-induced amnesia and hypermotility in the rat. European Journal of Pharmacology, 426(3): 193-200.
Rahimzadeh, M. R., Rahimzadeh, M. R., Kazemi, S., Amiri, R. J., Pirzadeh, M. and Moghadamnia, A. A. (2022). Aluminum poisoning with emphasis on its mechanism and treatment of intoxication. Emergency Medicine International, 2022:13.
Saiyed, S. M. and Yokel, R. A. (2005). Aluminium content of some foods and food products in the USA, with aluminium food additives. Food Additives and Contaminants, 22(3): 234-244.
Sallam, S. M. A., Nasser, M. E. A., Yousef, M. S. H., El-Morsy, A. M., Mahmoud, S. A. S. and Yousef, M. I. (2005). Influence of aluminum chloride and ascorbic acid on performance, digestibility, caecal microbial activity and biochemical parameters of rabbits. Research Journal of Agriculture and Biological Sciences, 1: 10-16.
Sofowora, A. (1993). Phytochemical screening of medicinal plants and traditional medicine in Africa, Spectrum Books Limited: pp 150–156.
Sorond, F. A., Lipsitz, L. A., Hollenberg, N. K. and Fisher, N. D. (2008). Cerebral blood flow response to flavanol-rich cocoa in healthy elderly humans. Neuropsychiatric Disease and Treatment, 4(2): 433-440.
Taïr, K., Kharoubi, O., Taïr, O. A., Hellal, N., Benyettou, I. and Aoues, A. (2016). Aluminium-induced acute neurotoxicity in rats: Treatment with aqueous extract of Arthrophytum (Hammada scoparia). Journal of Acute Disease, 5(6): 470-482.
Usen, D. U. and Enogieru, A. B. (2023). Aluminium chloride-induced prefrontal cortex toxicity in wistar rats: evidence from pretreatment with aqueous Psidium guajava leaf extract. Dutse Journal of Pure and Applied Sciences, 9(3b): 1-10.
Yuan, C. Y., Lee, Y. J. and Hsu, G. S. W. (2012). Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. Journal of Biomedical Science, 19(1): 1-9
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