Curative Influence of Natural Honey Bee on Liver Function Parameters in Aluminium Nitrate-induced Wistar Rats

Authors

DOI:

https://doi.org/10.2659/njbmb.2022.86

Abstract

The search for antidotes for Al toxicity is on-going. Thus, this study investigated the curative influence of natural honey bee on liver function parameters in aluminium nitrate-induced Wistar rats. Thirty (30) Wistar rats (150±6g) were divided into six groups: Group one served as control and received only feed and water. Groups 2-6 served as the test groups and received single dose of Al(NO3)3 (6.5 mg/kg body weight). Groups 3-6 received varying doses of NBH (10 %, 25 %, 50 % diluted with distilled water and 100 % natural BH orally at a dose of 2.5 g/kg respectively) 24 h after Al(NO3)3 intoxication for 14 consecutive days. The activities of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase in the plasma and liver and the levels of total protein, albumin and globulin were evaluated. Exposure to aluminium significantly (p< 0.05) increased the activities of ALT, AST and ALP relative to rats in the control group. However, the treatment of Al-exposed rats with varying doses of NBH significantly reduced the activities of the enzymes, compared to rats maintained on Al alone, with the exception of Al-exposed rats given 100 % NBH. Albumin concentrations were significantly higher in control rats than in rats exposed to Al. However, the administration of 50% NBH to Al-exposed rats significantly (p< 0.05) increased albumin concentration relative to rats exposed to Al alone. This study showed that NBH has significant positive effects on aluminum toxicity in rats with the 50 % NBH treatment having the greatest effect.

Downloads

Download data is not yet available.

References

Abdel-Moneim, A. E., Othman, M. S. Mohmoud, S. M. and El-Deib, K. M. (2013). Pomegranate peel attenuates aluminum-induced hepatorenal toxicity. Toxicology Mechanisms and Methods, 23(8): 624–633.

Abdel-Wahab, W. M. (2012). AlCl3-induced toxicity and oxidative stress in liver of male rats: protection by melatonin. Life Sciences, 9(4): 1173–1182.

Achuba, F. I. and Nwokogba, C.C. (2015). Effects of honey supplementation on hydrocarbon-induced kidney and liver damage in Wistar albino rats. Biokemistri, 27(1): 50–55.

Akpanyung, E. O., Nwaokonko, D. U., Ekong, M. B. and Ekpo, M. M. (2018). Evaluation of the protective effect of Moringa oleifera leaf extract against aluminium induced liver damage in male albino Wistar rats. International Journal of Sciences, 7(2): 21-31.

Al Dera, H. S. (2016). Protective effect of resveratrol against aluminum chloride induced nephrotoxicity in rats. Saudi Medical Journal, 37(4): 369–378.

Al-Kahtani M. and Morsy, K. (2019). Ameliorative effect of selenium nanoparticles against aluminum chloride-induced hepatorenaltoxicity in rats. Environmental Science and Pollution Research, 26(31): 32189–32197.

Al-Kahtani, M., Abdel-Daim, M. M., Sayed, A. A. El-Kott,A. and Morsy, K. (2020). Curcuminphytosome modulates aluminum-induced hepatotoxicity via regulation of antioxidant, Bcl-2, and caspase-3 in rats. Environmental Science and Pollution Research International, 27(17):21977–21985.

Al-Olayan, E. M., El-Khadragy, M. F., and Abdel Moneim, A. E. (2015). The protective properties of melatonin against aluminium-induced neuronal injury. International Journal of Experimental Pathology; 96(3):196–202.

Al-Qayim M., Ghali, L. and Al-Azwai, T. (2014). Comparative effects of propolis and malic acid on hematological parameters of aluminum exposed male rats. Global Journal of Bioscience and Biotechnology, 3(1):6–11

Alqayim, M.A.J. (2015). Propolis cardioprotective role from the impact of aluminium chloride in female rabbits. Basic Journal of Veterinary Research,14(2):136-149.

Al-Waili, N. S., Saloom, K. Y., Akmal, M., Al-Waili, F., Al-Waili, T. N., Al-Waili, A. N. and Ali, A. (2006). Honey ameliorates infliuence of hemorrhage and food restriction on renal and hepatic functions, and hematological and biochemical variables. International Journal of Food Science and Nutrition, 57(5–6):353–362.

Annino, J. S. and Giese, R. W. (1976) Clinical chemistry, principles and procedures, 4th edition, Little Brown and Company, Boston. pp. 76-82.

Bakour, M., Al-Waili, N. S., El Menyiy, N., Imtara, H., Figuira, A. C., Al-Waili, T. andLyoussi, B. (2017). Antioxidant activity and protective effect of bee bread (honeyand pollen) in aluminum-induced anemia, elevationof inflammatory makers and hepato-renal toxicity. Journal of Food Science and Technology, 54(13): 4205–4212.

Bhasin, P., Singla, N. and Dhawan, D. K. (2014). Protective role of zinc during aluminum‐induced hepatotoxicity. Environmental Toxicology, 29(3): 320-327.

Blasa, M. Candiracci, M. Accorsi, A. Piacentini, M. P. Albertini, M. C.and Piatti, E. (2006). Raw Millefiori honey is packed full ofantioxidants.Food Chemistry, 97(2): 217–222.

Bulan, Ö. K. Bayrak, B. B. Sarikaya-Ünal, G. and R. Yanardağ (2019). The influence of melatonin supplementation againstaluminum-induced toxicity in brains of male rats. Journal of Research in Pharmacy, 23(2): 275–283.

Cheraghi, E. and Roshanaei, K. (2019). The protective effect of curcumin against aluminum chloride-induced oxidative stress and hepatotoxicity in rats. Pharmaceutical and Biomedical Research, 5(1): 6–13.

Dass, A. P. and Ramoji, P.C. (2017). The effect of aqueous ginger extract on aluminium chloride (AlCl3) induced alteration in lipid profile of male Wister rats. International Journal of Basic and Clinical Pharmacology, 6: 1-4.

Doumas, B. T., Watson, W. A. and Biggs, H. G. (1971). Albumin standards and the measurement of serum albumin with bromcresol green. Clinica Chimica Acta, 31(1): 87-96.

Ekakitie, L. I., Okpoghono, J., Orororo, O. C. and Ekakitie, O. A. (2021). Ameliorative prowess of bee honey in the tissues of rats administered aluminium nitrate. Scientific African, 12, e00782.

El Rabey, H. A.,Al-Seeni, M. N. and Al-Solamy, S.M. (2013). Bees’ Honey Protects the Liver of Male Rats againstMelamine Toxicity. BioMed Research International Volume 2013, Article ID 786051, 8 pages.

El-Khayat Z. and Ahmed, H. H. (2000). Antitumer efficacy of edible Portulacaoleracea and bees honey in mice inoculated with Ehrlich ascttestumer cells. Journal of Union of Arab Biologists, 13: 583–605.

Erejuwa, O. O., Sulaiman, S. A. and AbWahab, M. S. (2012). Honey: a novel antioxidant. Molecules, 17(4):4400–4423.

Fiorani, M. Accorsi, A. Blasa, M. Diamantini, G. and Piatti, E. (2006). Flavonoids fromItalianmultifloral honeys reduce the extracellular ferricyanide in human red blood cells. Journal of Agricultural and Food Chemistry, 54(21): 8328–8334.

Lentini, P. Zanoli, L. Granata, A. Signorelli, S. S. Castellino, P. and Dellaquila, R. (2017). Kidney and heavy metals - the role of environmental exposure (review). Molecular Medicine Reports, 15(5): 3413–3419.

Oda, S. S. (2016). The influence of Omega3 fatty acids supplementation against aluminum-induced toxicity in male albino rats. Environmental Science and Pollution Research International, 223(14): 14354–14361

Okail, H. A., Ibrahim, A. S. and Badr, A. H. (2020). The protective effect of propolis against aluminum chloride-induced hepatorenal toxicity in albino rats. The Journal of Basic and Applied Zoology, 81(1): 1-11.

Othman, M. S., Fareid, M. A., Abdel Hameed, R. S. and Abdel Moneim, A. E. (2020). The protective effects of melatonin on aluminum-induced hepatotoxicity and nephrotoxicity in rats. Oxidative Medicine and Cellular Longevity, 2020, Article ID 7375136, 12 pages.

Reitman, S. and Frankel, S. A. (1957). Colomentric method for determination of serum glutamic oxalacetic acid and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28: 56–63.

Sharma, D. R., Wani, W. Y., Sunkaria, A., Kandimalla, R. J., Sharma, R. K., Verma, D., Bal, A. and Gill, K. D. (2016) Quercetin attenuates neuronal death against aluminum-induced neuro degeneration in the rat hippocampus. Neuroscience, 324: 163–176.

Shati, A. A. and Alamri, S. A. (2010). Role of saffron (Crocus sativus L.) and honey syrup on aluminum-induced hepatotoxicity. Saudi Medical Journal, 31(10): 1106–1113.

Shrivastava, S. (2013). Amelioration of aluminium induced toxicity by Allium sativum. Scientific Research and Assays, 8(4): 168-177.

Sivakumar, S., Khatiwada, C. P. andSivasubramanian, J. (2012). Bioaccumulations of aluminum and the effects ofchelating agents on different organs of Cirrhinusmrigala. Environmental Toxicology and Pharmacology, 34(3): 791-800.

Tietz, N. W. (1999). Fundamental of clinical chemistry. 3rd ed. Carl AB, Edward RA (ed.). W. B. Saunders, Co,

Turgut, G., Kaptanoglu, B., Enli, Y. and Genc, O. (2004). Effect of chronic aluminum administration on blood and liver iron related parameters in mice. Yonsei Medical Journal, 45(1): 135-9

Wang, X. Gong, J. Gui, Z. Hu, T. and Xu, X. (2018). Halloysitenanotubes-induced Al accumulation and oxidative damagein liver of mice after 30-day repeated oral administration. Environmental Toxicology, 33(6): 623–630.

WHO (2010). Aluminium in drinking water. Geneva Switzerland, p. 1.

Yakubu, O.E., Nwodo, O.F. C., Imo, C., Abdulrahaman, M. and Uyeh, L. B. (2016). Effects of Vitexdoniana leaf extract on aluminium induced toxicity in male albino Wistar rats. Journal of Applied Biology and Biotechnology, 4(5): 37-40

Yousef, M. I. (2004). Aluminium-induced changes in hemato-biochemicalparameters, lipid peroxidation and enzyme activities of male rabbits: Protective role of ascorbic acid. Toxicology, 199(1): 47–57.

Yousef, M. I. Mutar, T. F. and. Kamel, M. A. E. L. N (2019). Hepatorenal toxicity of oral sub-chronic exposure to aluminum oxide and/or zinc oxide nanoparticles in rats. Toxicology Reports, 6: 336–346.

Zhu, W., Jia, Q., Wang, Y., Zhang, Y., and Xia, M. (2012). The anthocyanincyanidin-3-O-b-glucoside, a flavonoid, increases hepatic glutathione synthesis and protects hepatocytes against reactive oxygen species during hyperglycemia: Involvement of a cAMP-PKA-dependent signaling pathway. Free Radical Biology and Medicine, 52: 314–327.

Published

2022-10-05

How to Cite

Ekakitie, L. I. ., Orororo, O. C. ., & Okpoghono, J. . (2022). Curative Influence of Natural Honey Bee on Liver Function Parameters in Aluminium Nitrate-induced Wistar Rats. Nigerian Journal of Biochemistry and Molecular Biology, 37(3), 175–180. https://doi.org/10.2659/njbmb.2022.86

Issue

Section

Research Articles

Categories