Assessment of Groundnut Milk Intake on Nutritional Status and some Biochemical Indices of Normal Rats


  • Muazu G. Aliyu Department of Biochemistry, Bayero University, Kano, Nigeria
  • Adamu J. Alhassan Department of Biochemistry, Bayero University, Kano, Nigeria
  • Abbas Sani Department of Biochemistry, Bayero University, Kano, Nigeria
  • Abba Babandi Department of Biochemistry, Bayero University, Kano, Nigeria
  • Abdullahi M. Umar Department Integrated Science, Federal College of Education, Kano, Nigeria
  • Kamaluddeen Babagana Department of Biochemistry, Bayero University, Kano, Nigeria


Groundnut milk, Biochemical parameters, Organogenic indices


Groundnut milk also known as peanut milk, is an increasingly popular based beverage that serves as a potential alternative to cow’s milk. It is rich in essential nutrients such as proteins, healthy fats, vitamins, and minerals but limited research has explored its effect on nutritional status and biochemical indices. Therefore, this study assessed the effect of groundnut milk intake on nutritional status and some biochemical indices of Wistar rats. The Wistar rats (n=20), with body weight ≤50g were randomly divided into four groups of five rats each: Group 1 served as control. Groups 2, 3, and 4 were administered groundnut milk at daily doses of 247.2, 494.3, and 988.6 mg/kg respectively for eight weeks. Significant increase in abdominal circumference (P<0.005) between groups was observed. However, increase in the BMI and thoracic circumference between groups were insignificant (P>0.05). The levels of TC, TG and LDL-c were decreased significantly (P<0.005). However, HDL-c showed no significant difference. The decrease in atherogenic index, atherogenic coefficient and cardiac risk ratio indices were insignificant (P>0.05). The groundnut milk had no effect on serum total protein, adiponectin, and albumin while it increased serum leptin level significantly (P<0.005). The blood glucose levels of group 2, 3 and 4 were lowered dose-dependently (P<0.005) compared to the control. Groundnut milk showed no effect on liver and kidney-to-body weight ratio (P>0.05), but showed effect on heart-to-body weight ratio (P<0.005). The ratio of food and water intake of groups 2, 3, and 4 decreased significantly (P<0.005) compared to the control group.


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Aguwa, U.S., Okeke, S.N., Eze C. and Ovie F. (2020). Evaluating methods of rat euthanasia on the liver and kidney of wistar rats: cervical dislocation, chloroform inhalation, diethyl ether inhalation and formalin inhalation. Letters in Health Biological Science, 5(1), 8-13.

Ajeigbe, H.A., Waliyar, F., Echekwu, C.A., Ayuba, K., Motagi, B.N., Eniayeju, D. and Inuwa A. (2014). A farmer’s guide to groundnut production in Nigeria. Patancheru, 502, 324,

Alhassan, A. J. Sule, M. S., Atiku, M. K., Wudil, A. M., Abubakar, H. and Mohammed, S. A. (2012). Effects of aqueous avocado pear (Perseaamericana) seed extract on alloxan induced diabetes rats. Greener Journal of Medical Sciences; 2 (1), 005-011.

Altura, B. T., Brust, M., Bloom, S., Barbour, R. L., Stempak, J. G., & Altura, B. M. (1990). Magnesium dietary intake modulates blood lipid levels and atherogenesis. Proceedings of the National Academy of Sciences, 87(5), 1840-1844.

Amit, G., Vandana, S. and Sidharth, M. (2011). Hyperlipidemia: An updated review. International Journal of Biopharmaceutics and Toxicological Research. 1, 81-89.

AOAC, (2000). Official Method of Analysis. Association of Official Analytical Chemists, 17th Ed.; Gaithersburg, MD, USA.

Atama, J.E and Idu, M. (2007). Histopathologic effects of mathanolic extract of Momordica charantia leaves on the liver of Wister rats. Trends in Medical Research Journal, 2(4), 176-184.

Awad, A.B., Chan, K.C., Downie, A.C. and Fink, C.S. (2000). Peanuts as a source of â-sitosterol, a sterol with anticancer properties. Nutrition and Cancer; 36(2), 238–41.

Bao, Y., Han, J., Hu, F. B., Giovannucci, E. L., Stampfer, M. J., Willett, W. C. and Fuchs, C. S. (2013). Association of nut consumption with total and cause-specific mortality. New England Journal of Medicine; 369(21), 2001–2011.

Barnea, M., Shamay, A., Stark, A.H. and Madar, Z. (2006). A high-fat diet has a tissue-specific effect on adiponectin and related enzyme expression. Obesity (Silver Spring); 14 (12):2145-53.

Bhat, E. A, Sajjad, N, Manzoor, I. and Rasool A. (2019). Bioactive Compounds in Peanuts and Banana. Biochemistry and Analytical Biochemistry; 8(382), 382.

Cowley, M.A., Smart, J.L., Rubinstein, M., Cerdan, M.G., Diano, S., Horvath, T.L., Cone, R.D. and Low, M.J. (2001). Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus. Nature; 411: 480-484.

Ho, C. T., Mussinan, C., Shahidi, F., & Contis, E. T. (Eds.). (2010). Recent advances in food and flavor chemistry: food flavors and encapsulation, health benefits, analytical methods, and molecular biology of functional foods.

Facey A., Dilworth L. and Irving R. (2017). A Review of the Leptin Hormone and the Association with Obesity and Diabetes Mellitus. Journal of Diabetes Metabolism; 8, 727.

FAO (1998). Crop evapotranspiration. Irrigation and drainage paper, Rome, 56.

Fiona, M., Phoebe, L., Anna, K., Regiane, L. S., Neuza, M. B. C., Josefina, B., Rita, C. G. A. and Richard, D. M. (2010). Effects of peanut processing on body weight and fasting plasma lipids. British Journal of Nutrition; 104(3), 418–426.

Foley, Y. J., Buckley, J. and Murphy, M. F. (1974). Commercial testing and product control in the dairy industry. University College, Cork.

Gebauer, S.K., Novotny, J.A., Bornhorst, G.M. and Baer, D.J. (2016). Food processing and structure impact the metabolizable energy of almonds. Food and Functions; 7 (10), 4231–4238.

Gonzalez, C. A. and Salas-Salvado, J. (2006). The potential of nuts in the prevention of cancer. British Journal of. Nutrition; 96(S2), S87–S94.

Gornall, A. G., Bardawill, C. J., & David, M. M. (1949). Determination of serum proteins by means of the biuret reaction. Journal of biological Chemistry, 177(2), 751-766.

Harding, J. R., & Keyser, J. W. (1968). Bromocresol green as a reagent for serum albumin. Proceedings of the Association of Clinical Biochemists, 5(2), 51-53.

Hasina, A., Nasim, J. and Nayma, S. (2015). Effect of peanut (Arachis HypogaeaL.) on dyslipidemia in young adult. Journal of Bangladesh Society of Physiologist, 10(1), 11-16.

Hession, M., Rolland, C., Kulkarni, U., Wise, A. and Broom, J. (2009) “Systematic review of randomized controlled trials of low carbohydrate vs. low-fat/low-calorie diets in the management of obesity and its comorbidities,” Obesity Reviews, 10(1), 36–50.

Hofman, P.J., Vuthapanich, S., Whiley, A.W., Klieber, A. and Simons, D.H. (2002). Tree yield and fruit minerals concentrations influence “Hass” avocado fruit quality. Science Horticulture; 92: 113–123

Hu, F.B., Stampfer, M.J, Manson, J.E. and Willett, W.C. (1998). Frequent nut consumption and risk of coronary heart disease in women prospective cohort study. British Medical Journal; 317(14): 1341–45.

Ibrahim, S. I., Ameh, D.A., Atawodi, S.E. and Umar, I.A. (2016). Carbonic anhydrase: a new therapeutic target for managing diabetes. Journal of Metabolic Syndrome, 5(1), 1000196.

Izadi, V., Farabad, E. and Azadbakht, L. (2013). Epidemiologic evidence on serum adiponectin level and lipid profile. International Journal of Preventive Medicine; 4(2), 133-140.

Janice, R.H. (2019). Protein and the body. Oklahoma cooperative extension fact sheets:

Jennette, H. (2005). The potential role of peanuts in the prevention of obesity. Nutrition & Food Science: 35(5),353-358.

Joan, S.M.D., Keiji, O. M.A. and Emilio, R.M.D. (2010). Nut consumption and blood lipid levels; a pooled analysis of 25 intervention trials. Archives of Internal Medicine; 170(9), 821-827.

Jones, J. B., Provost, M., Keaver, L., Breen, C., Ludy, M. J. and Mattes, R. D. (2014). A randomized trial on the effects of flavorings on the health benefits of daily peanut consumption. American Journal of clinical Nutrition; 99(3), 490–496.

Khan, S.A, Qurrat-ul-Ain, K.M., Khan M., Muhammad, S., and Ghayas, R. (2017,). Dry fruits and diabetes mellitus. International Journal of Medical Research & Health Sciences; 6(4), 116-119. 116.

Kirkmeyer, S. and Mattes, R.D. (2000). Effects of food attributes in hunger and food intake. International Journal of Obesity; 24, 1167-75.

Kjeldahl, J. (1883). Determination of protein nitrogen in food products. Encyclopedia of Food. Sciences, 439 - 441.

Kris-Etherton, P.M., Pearson, T.A., Wany, Y., Hargrave, R.L., Moriarty, K. and Fishell, V. (1999). High monounsaturated fatty acid diets lower both plasma cholesterol and triacylglycerol concentration. American Journal of Clinical Nutrition; 70(6), 1009-15.

Leticia, F., De Felice, F. G. and Vieira, M. N. (2019). The role of leptin and adiponectin in obesity-associated cognitive decline and alzheimer’s disease. Frontiers in Neuroscience; 12, 1027.

Maeda, N., Shimomura, I., Kishida, K… and Matsuzawa, Y. (2002). Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nature Medicine; 8:731–737.

Martin, G., Myers, M., Cowley A. and Heike, M. (2008). Mechanisms of Leptin Action and Leptin Resistance. Annual Review Physiology.70:537-556.

Mohammed, A., Wudil, M., Alhassan, A. J., Imam, A.A., Muhammad, I. U. and Idi, A. (2017). Hypoglycemic activity of Curcuma longa Linn root extracts on alloxan induced diabetic rats. Saudi Journal of Life Science; 2(2), 43-49.

Moreno, J. P., Johnston, C. A., El-Mubasher, A. A., Papaioannou, M. A., Tyler, C., Gee, M. and Foreyt, J. P. (2013). Peanut consumption in adolescents is associated with improved weight status. Nutrition Research33(7), 552–526.

Nam, E. K., Ae, W. H., Hye, W. W. and Woo, K. K. (2014). Peanut sprouts extract (Arachis hypogaea) has anti-obesity effects by controlling the protein expressions of PPARγ and adiponectin of adipose tissue in rats fed high-fat diet. Nutrition Research and Practice; 8(2), 158-164.

Novelli, E. L. B., Diniz, Y. S., Galhardi, C. M., Ebaid, G. M. X., Rodrigues, H. G., Mani, F., ... & Novelli Filho, J. L. V. B. (2007). Anthropometrical parameters and markers of obesity in rats. Laboratory animals, 41(1), 111-119.

O’Connor, C. B. (1994). Rural Dairy Technology. ILCA Training manual. International Livestock Research Institute, Addis Ababa, Ethiopia pp133.

Okafor, O. E., Ezeanyika, L. U. S., Nkwonta, C. G. and Okonkwo, C. J. (2015). Plasma lipid profiles and atherogenic indices of rats fed raw and processed jack fruit (Artocarpusheterophyllus) seeds diets at different concentrations. International Scholarly and Scientific Research & Innovation; 9(8). 885-889.

Okafor, P. N., Nwankpa, P., Ekweogu, C. N., Etteh, C. C. and Ugwueznmba, P.C, (2018) Assessment of the weight reducing potentials of ethanolic seed extract of cola lepidota k schum in high fat fed female albino wistar rats. American Journal of Phytomedicine and Clinical Therapeutics; 6(3),14. ISSN 2321-2748.

Omole, J. O., and Ighodaro, O. M. (2012). Proximate composition and quality attributes of milk substitute from melon seeds (Citrulus vulgaris schrad). Magnesium, 16(2), 5.

Perugu, B. Y., Patel, S. L. E. and Bhaskara D. R (2018). Comparison of the proximate composition of peanut milk and milk powder using different methods. International Journal of Chemical Studies, 6(5), 2424-2427.

Phoebe, L., Anna, L., Margaret, A., Richard, D. and Mattes, R. D. (2007). Regular peanut consumption improves plasma lipid levels in healthy Ghanaians. International Journal of Food Sciences and Nutrition; 58 (3), 190-200.

Rabiu, A. M., Wale, H., Garba, K., Sabo, A. M., Hassan, Z., Shugaba, A. I., ... & Odeh, S. O. (2017). Body mass index of male and female Wistar rats following administration of leptin hormone after a dietary regime. Annals of Bioanthropology, 5(1), 22.

Regiane, L., Sales, S. B., Coelho, Neuza M. B., Costa, J. B., Swaminathan, I., Linda, A. B., Phoebe, L. and Richard, D.M. (2008). The effect of peanut oil on lipid on lipid profile of normolipidemic adults: A three country collaborative study. Journal of Applied Research; 8(3), 216-225.

Semrad, C.E. (2012). Approach to the patient with diarrhea and malabsorption. Goldmanscecil medicine. 2012:895-913.

Shalini S. Arya. Akshata R. Salve and S. Chauhan (2016). Peanuts as functional food: A Review Journal Food Science Technology. 53(1), 31–41.

Sze-Yen, T., Siew L. T. and Rachel B. (2018). Can nuts mitigate malnutrition in older adults? A conceptual framework. Nutrients; 10: 1448

Tobin West, M. D. and Baraka, R. E. (2018). Effects of methods of processing groundnut (Arachis hypogaea) on the nutritional composition and storage life of the seed. International Journal of Agriculture and Earth Science; 4(6), 43-49.

Usoro, C. A. O., Adikwuru, C. C., Usoro, I. N., &Nsonwu, A. C. (2006). Lipid profile of postmenopausal women in Calabar, Nigeria. Pakistan Journal of Nutrition, 5(1), 79-82.

Xue-Dong, C., Yong-Feng, W., Yu-Long, W., and Shi-Qing, X. (2018). Induced hyperproteinemia and its effects on the remodeling of fat bodies in silkworm, bombyx. Frontiers in Physiology, 9;302

Zhu, L., Luo, X. and Jin, Z. (2008). Effect of resveratrol on serum and liver lipid profile and antioxidant activity in hyperlipidemia rats. Asian-Australian Journal of Animal Science; 21(6), 890 – 95.



How to Cite

Aliyu, M. G., Alhassan, A. J., Sani, A., Babandi, A., Umar, A. M., & Babagana, K. (2023). Assessment of Groundnut Milk Intake on Nutritional Status and some Biochemical Indices of Normal Rats. Nigerian Journal of Biochemistry and Molecular Biology, 38(4), 172–183. Retrieved from