Bioassay Guided Evaluation and Characterization of Solanum macrocarpon Fruits and Leaf Fractions via In Vitro Antiglycation, Radical Scavenging and Molecular Docking Analyses
DOI:
https://doi.org/10.4314/njbmb.v40i1.6Keywords:
Antiglycation , Radical Scavenging, GC-MS, Molecular Docking, Solanum macrocarponAbstract
Plants, both in their natural state and as extracts/fractions, have provided significant benefits to human health for centuries. The pharmacological benefits of these plants is attributed to the diversity of primary and secondary metabolites. This study evaluated the in vitro DPPH scavenging capacity, antiglycation activity and bioactive constituents of Solanum macrocarpon fruit and leaf fractions using Chromatographic, Spectrophotometric, Spectro-fluorescence, Spectrometry assays and Molecular docking methods. Results revealed that ethylacetate fruit fraction exhibited the highest activity overall, regarding both radical scavenging and antiglycation activities. Such potent dual bioactivity can be ascribed to the presence of some prominent compounds identified from GC-MS analysis, which includes tetradecanoic acid, n-hexadecanoic acid and cycloeicosane. Molecular docking studies highlighted five compounds (methoxyacetic acid, 3-pentadecyl ester; tetradecanoic acid, n-hexadecanoic acid, cycloeicosane- and 3-eicosene, (E)-) having a pronounced affinity with bovine serum albumin (BSA), indicating their potential to impede binding of AGEs to BSA. Findings from this study encourages further investigations on other biological activities of this plant besides its antioxidant and antiglycation activity.
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Copyright (c) 2025 Hauwa S. Usman, Umar A. Umar, Funmilola Audu, Mukhtar A. Suleiman, Aishat Yakubu, Abdullahi B. Sallau (Author)
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