Antioxidant and Enzyme Inhibition Properties of Okra-Orange (Abelmoschus esculentus–Citrus sinensis) Juice Blend: Development, Stability, and Potential Function
DOI:
https://doi.org/10.4314/njbmb.v40i1.2Keywords:
Antioxidant, Okra-Orange juice blend, Functional beverageAbstract
Functional beverages are a sort of drinks that are free of alcohol but contain bioactive components from plant or microorganism that contribute to strengthening of human health. This work evaluates the antioxidant and antihyperglycemic potential of a functional beverage developed from okra (Abelmoschus esculentus) orange (Citrus sinensis) juice blend. Orange juice and okra fruit juice were prepared and was blended in the following ratios 98:2 (2%), 96:4 (4%), 94:6 (6%), 92:8 (8%), 90:10 (10%) and control 100:0 (0%) respectively. The okra-orange juice blend was also stored for 14 days. The total phenolic content (TPC), Total flavonoid content (TFC), Antioxidant Capacity, α-amylase and α-glucosidase inhibitory activities were assessed. The total phenol and flavonoid content of orange juice increased significantly (P < 0.05) from 135.8 mg (GAE) / g to 231.6 mg (GAE) / g and 45.6 mg QE / g to 100.0 mg QE / g respectively as the percentage of okra juice added increased. In storage, the total phenol and flavonoid content of the okra-orange juice blend was significantly higher than the orange juice at different storage days and they were stable. The juice blend scavenged DPPH and ABTS radicals, the glucose enzyme inhibitory activity increased from 23.9% to 75.4%; α-amylase and 11.4% to 63.7% α-glucosidase enzymes, these activities increased directly as the added okra juice percentage increased. Okra-orange juice blend has the potential of stabilizing free radicals, control postprandial blood glucose level and be used in formulating functional beverage for the mitigation of hyperglycemia.
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Copyright (c) 2025 Foluso O Adetuyi, Emmanuel S. Akintimehin, Kayode O. Karigidi, Peter A. Makinde, Samuel T. Omogunwa, Faith E Ogunbameru, Anuoluwapo P. Fapetu (Author)
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