In vitro Antioxidant Activities and Blood Protective Effects of Aqueous Extracts of Xylopia aethiopica L. Whole Seed and Pod

Authors

  • Habibu Tijjani Bauchi State University Gadau https://orcid.org/0000-0001-5466-322X
  • Aliyu Abdulhamid Omar
  • Abubakar Mohammed
  • Fatima Muhammad Habibu
  • Yohanna Benjamen Maina
  • Ali Musa

DOI:

https://doi.org/10.2659/njbmb.2022.8

Keywords:

Xylopia aethiopica, Antioxidant activities, Plasma, Oxidation, whole Seed, Pod, GCMS analysis

Abstract

Xylopia aethiopica L. (Annonaceae) is medicinally important in the treatment of a wide range of diseases. In Nigeria, X. aethiopica whole seed are locally used in the treatment of constipation. They are used as a tonic tea and beverages, and after pregnancy delivery to facilitate the removal of clotting blood in the system. The study evaluated in vitro antioxidant activities and blood protective effects of X. aethiopica aqueous extracts. The 2,2-diphenyl-1-picrylhydrazyl scavenging activities of the seed (IC50 = 0.19±0.03 mg/mL) and pod (IC50 = 0.24±0.02 mg/mL) extract were not significantly (p>0.05) different with butylated hydroxytoluene BHT (IC50 = 0.17±0.04 mg/mL) and ascorbic acid (IC50 = 0.13±0.05 mg/mL). Furthermore, total antioxidant capacity and nitric oxide reducing power of the seed, pod and whole seed were similar with no significant difference (p>0.05) when compared with BHT. However, the seed (IC50 = 0.89±0.09 mg/mL), pod (IC50 = 0.35±0.03 mg/mL) and whole seed (IC50 = 0.32±0.03 mg/mL) expressed significantly (p<0.05) lower hydrogen peroxide decomposing activity when compared with BHT (IC50 = 0.15±0.00 mg/mL) and ascorbic acid (IC50 = 0.15±0.01 mg/mL). The extract expresses no erythrocyte lysis activities at a concentration range of 6.25 – 100 µg/mL and protect against accumulation of CuSO4-induced conjugated dienes in plasma. The present study demonstrated the protective nature of X. aethiopica L. seed, pod and whole seed extracts against the accumulation of conjugate dienes, thrombolytic activities and membrane stability effects.  X. aethiopica L. is recommended for consideration as a significant natural antioxidant source.

References

Adom, K.K. and Liu, R.H. (2005). Rapid peroxyl radicalscavenging capacity (PSC) assay for assessing both hydrophilic and lipophilic antioxidants. Journal of Agricultural and Food Chemistry, 53 (17): 6572–6580.

Ahrens, T., Rutherford, K. and Basham, K.A.R.(1993). Essentials of oxygenation: implication for clinical practice. Jones and Bartlett Publishers.

Ali, M.R., Kuri, S., Das, A. and Islam, M.A. (2013). Preliminary phytochemical screening and in vitro thrombolytic potential of the methanolic extract of Enhydra fluctuans Lour (leaves). International Journal of Pharmamedix India, 1: 270- 280.

Amudha, M. and Rani, S. (2016). Evaluation of in vitro antioxidant potential of Cordia retusa. Indian Journal of Pharmaceutical Science, 78(1): 80–86.

Aruoma, O. I. (1998). Free radicals, oxidative stress, and antioxidants in human health and disease. Journal of the American Oil Chemists Society, 75 (2): 199-212.

Bick, R.L. and Kaplan, H. (1998). Syndromes of thrombosis and hypercoagulability: congenital and acquired causes of thrombosis. Medical Clinics of North America, 82: 409–458.

Bosman, J., Tangelder, G.J., Egbrink, M.G., Reneman, R.S. and Slaaf, D.W. (1995). Red blood cell flow cessation and diameter reductions in skeletal muscle capillaries in vivo - the role of oxygen. Pflugers Archiv : European Journal of Physiology, 430(5): 852–861.

Burkill, H. (1995). The useful plants of west tropical Africa. Families JL. Kew. Royal Gardens kew xi ISBN 1995;3:857.

Chebouat, E., Dadamoussa, B., Gharabli, S., Gherraf, N., Allaoui, M., Cheriti, A., Lahham, A. and and Zellagui, A. (2011). Assessment of antimicrobial activity of flavonoids extract from Ephedra alata. Der Pharmacia Lettre, 6(3):27-30.

Cheung, L.M., Cheung, P.C. andOoi, V.E.(2003). Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry, 81: 249−255.

Doggen, C.J., Smith, N.L., Lemaitre, R.N., Heckbert, S.R., Rosendaal, F.R. and Psaty, B.M.(2004). Serum lipid levels and the risk of venous thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 24:1970–5.

Dröge, W. (2002). Free radicals in the physiological control of cell function. Physiological Reviews, 82(1): 47–95.

Eze, K.N. (2012). Antifertility effects of ethanolic extract of Xylopia aethiopica on male reproductive organ of wistar rats. American Journal of Medicine and Medical Sciences, 2(1): 12-15.

Fiorentino, A., Ricci, A., D’Abrosca, B., Pacifico, S., Golino, A., Letizia, M., et al. (2008). Potential food additives from Carexdistachya roots: Identification and in vitro antioxidant properties. J Agric Food Chem., 56:8218-25.

Furie, B. and Furie, B.C. (2008). Mechanisms of thrombus formation. The New England Journal of Medicine, 359: 938–949.

Girasole, M., Pompeo, G., Cricenti, A., Congiu-Castellano, A., Andreola, F., Serafino, A., Frazer, B.H., Boumis, G. and Amiconi, G. (2007). Roughness of the plasma membrane as an independent morphological parameter to study RBCs: a quantitative atomic force microscopy investigation. Biochimica et Biophysica Acta, 1768: 1268-1276.

Gülçin, I. (2012). Antioxidant Activity of Food Constituents: An Overview. Archives of Toxicology, 86 (3): 345–91.

Halliwell, B, and Whiteman, M. (2004). Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? British Journal of Pharmacology, 142:231–55.

Jirovetz, L., Buchbauer, G. andNgassoum, M. (1997). Investigation of the essential oils from the dried fruits of Xylopia aethiopica (West African "Peppertree") and Xylopia parviflora from Cameroun. Ernährung/Nutrition, 21: 324-325.

Karioti, A., Hadjipavlou-Litina, D., Mensah, M.L., Fleischer, T.C. and Skaltsa, H. (2004). Composition and antioxidant activity of the essential oils of Xylopia aethiopica (Dun) A. Rich. (Annonaceae) leaves, stem bark, root bark, and fresh and dried fruits, growing in Ghana. Journal of Agricultural and Food Chemistry, 29;52(26):8094-8.

Katzung, B.G. (2008). Basic and clinical pharmacology. McGraw-Hill Education (Asia), 10th Edition. P. 545.

Koroch, A.R., Juliani, H.R. and Zygadlo, J.A. (2007). Bioactivity of essential oils and their components. In: Berger R.G (ed) flavours and fragrances. Chemistry, bioprocessing and sustainability. Springer-Verlag Berlin Heidelberg.

Kumar, R.S., Sivakuma, T., Sunderem, R.S., Gupta, M., Murugesh, K., Rajeshwa, Y., Kumar, M.S. and Kumar, K.A. (2005). Antioxidant and antimicrobial activities of Bauhinia recemosaL. stem bark. Brazilian Journal of Medical and Biological Research, 38: 1015 1024.

Liao, W., Ning, Z., Chen, L., Wei, Q., Yuan, E., Yang, J. and Ren, J. (2014). Intracellular antioxidant detoxifying effects of Diosmetin on 2,2- azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress through inhibition of reactive oxygen species generation. Journal of Agricultural and Food Chemistry, 62: 8648−8654.

Nabavi, S.M., Ebrahimzadeh, M.A., Nabavi, S.F., Fazelian, M. and Eslami, B. (2009). In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacognosy Magazine, 5:122-6.

Odukoya, O.A., Inya-Agba, S.I., Segun, F.I., Sofidiya, M.O. and Ilori, O.O. (2007). Antioxidant activity of selected Nigerian green leafy vegetables. American Journal of Food Technology, 2:169-175.

Olanlokun, J.O., Lawal, O., Babarinde, C.O. and Olorunsogo, O.O.(2020). erythrocyte membrane stabilisation, protease activities and antioxidant properties of the stem bark extract of Alstoniaboonei (DC). Archive of Basic and Applied Medicine, 8:35 – 44.

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. and Simons, A. (2009). Agroforestree database a tree reference and selection guide version 4.0.

Paraiso, L.F., Gonçalves, E., Oliveira, A.F.M., Cunha, L.M., De Almeida Neto, O.P., Pacheco, A.G., Araújo, K.B.G., GarroteFilho, M.S., Neto, M.B. and Penha-Silva, N. (2017). Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers. PLoS ONE, 12: e0171318.

Pham-Huy, L.A., He, H. and Pham-Huy, C. (2008). Free radicals, antioxidants in disease and health. International Journal of Biomedical Science : IJBS, 4(2), 89–96.

Prasad, S., Kashyap, R.S., Deopujari, J.Y., Purohit, H.J., Taori, G.M. andDaginawala, H.F. (2007). Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis. BMC Complementary and Alternative Medicine, 7:36.

Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269: 337-41.

Rathee, P., Chaudhary, H., Rathee, S., Rathee, D., Kumar, V. and Kohli, K. (2009). Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflammation and Allergy - Drug Targets, 8:229–35.

Rifici, V.A. and Khachadurian, A.K. (1996). Effects of dietary vitamin C and E supplementation on the copper mediated oxidation of HDL and on HDL mediated cholesterol efflux. Atherosclerosis, 127: 19−26.

Ruch, R.J., Cheng, S.J. and Klaunig, J.E.(1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10: 1003–1008.

Shinde, U.A., Phadke, A.S., Nair, A.M., Mungantiwar, A.A., Dikshit, V.J. and Saraf, M.N. (1999). Membrane stabilizing activity — a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70:251–7.

Sikder, M., Millat, M. and Sultana, A. (2012). In vitro membrane stabilizing activity total phenolic content, cytotoxic, thrombolytic and antimicrobial activities of Calliandrasurinamensis (Wall.). Journal of Pharmacognosy and Phytochemistry, 1:40–44.

Tatsadjieu, L. N., Essia Ngang, J. J., Ngassoum, M. B. and Etoa, F. X. (2003). Antibacterial and antifungal activity of Xylopia aethiopica, Monodora myristica, Zanthoxylum xanthoxyloides and Zanthoxylum leprieurii from Cameroon. Fitoterapia, 74(5), 469–472.

Tijjani, H., Egbuna, C. and Luka, C.D. (2018). Biosynthesis of phytochemicals, in phytochemistry, volume 1, chapter 2, fundamentals, modern techniques, and applications, Apple Academic Press Inc. Canada. Pp 37-78.

Turkoglu, A., Duru, M.E., Mercan, N., Kivrak, I. and Gezer, K. (2007). Antioxidant and antimicroial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry. 101: 267–273.

Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M. and Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology, 39(1): 44–84.

Woode, E., Alhassan, A. and Chrissie, S.A. (2011). Effect of ethanolic fruit extract of Xylopia aethiopica on reproductive function of male rats. International Journal of Pharmaceutical and Biomedical Research, 2(3): 161-165.

Zou, C.G., Agar, N.S. and Jones, G.L. (2001). Oxidative insult to human red blood cells induced by free radical iniator AAPH and its inhibition by a commercial antioxidant mixture. Life Sciences, 69(1): 75-86.

Published

2022-03-01

How to Cite

Tijjani, H., Omar, A. A., Mohammed, A., Habibu, F. M., Maina, Y. B., & Musa, A. (2022). In vitro Antioxidant Activities and Blood Protective Effects of Aqueous Extracts of Xylopia aethiopica L. Whole Seed and Pod. Nigerian Journal of Biochemistry and Molecular Biology, 37(1), 48–57. https://doi.org/10.2659/njbmb.2022.8