The Leaf Extract of Carica papaya alleviates Benign Prostate Hyperplasia in male Albino rats by Antioxidative Mechanisms
Keywords:
Antioxidants, benign prostate hyperplasia, Inflammation, Carica papaya.Abstract
The pathology of many disorders in humans such as inflammation has been linked with free radical activities. This study was done to evaluate the potential ameliorative action of Carica papaya leaf extract on benign prostatic hyperplasia which is highly prevalent today. The animals were grouped randomly into six with fiverats in each group. Group 1 served as normal control, while groups (2–6) were induced with prostate inflammation using 0.08 mg/kg of testosterone and 0.04mg/kg of oestradiol, parenterally for 28 days. Group 2 and 3 served as positive and negative control. The test groups (4-6) were treated with 200, 400 and 800 mg/kg body weight of Carica papaya extract for another 28 days. At the end of the treatment period, blood samples were collected for required biochemical analysis; PSA, testosterone, Dihydrotestosterone (DHT), malondialdehyde, (MDA), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD). The results showed significant (p<0.05) increase in the serum PSA, testosterone, DHT and MDA levels of the animals exposed to the inducing agents, whereas there was a significant (p>0.05) decrease in the activities of GR, CAT and SOD as compared with the normal control. However, treatment with ethanol leaf extract of C. papaya caused significant (p >0.05) decrease in PSA, testosterone, DHT and MDA levels, and there was a significant (p>0.05) increase in the activities of the antioxidant enzymes in a dose dependent manner as comparable to the normal control. These findings are indication that the extract has potential remedial effects on benign prostatic hyperplasia through antioxidant mechanism.
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References
Aebi, H. E. (1983). Methods of Enzymatic Analysis. 3rd ed Florida; Weinheim Deerfield Beach: p 273- 285.
Anitha, B., Ragbu, N., Gopenath, T. S., Karthikeyan, M., Gnanasekaran, A., Chandrashekrappa, G. K. and
Basalingappa, K. M. (2018). Medicinal uses of Carica papaya. Journal of Natural and Ayurvedic Medicine, 2 (6), 1 – 11.
Aydin, A., Arsoro-Sarafinovska, Z., Sayal, A., Eken, A., Erdem, O., Erten, O., Ozgot, V. and Dimorski, A. (2006). Oxidative Stress and Antioxidant status in non-metastatic prostate cancer and benign prostatic hyperplasia. Clinical Biochemistry, 39(2), 176-179.
Chandra, K., Salman, A. S., Mohd, A., Sweety, R., & Ali, K. N. (2015). Protection against FCA induced oxidative stress induced DNA damage as a model of arthritis and In vitro anti-arthritic potential of costusspeciosus rhizome extract. International Journal of Pharmacognosy and Phytochemical Research, 7(2), 383-389.
Ekeyi, Y., Uchendu, N. O., Anaduaka, E. G. and Ezeanyika, L.U.S. (2021). Ethanol extract of Cassia sieberiana leaves ameliorates deviances associated with benign prostate hyperplasia in rats. All Life, 14(10, 473-483.
Foo, K. T. (2017). Pathophysiology of clinical benign prostatic hyperplasia. Asian Journal of Urology, 4(3), 152 – 157.
Fridovich, I. (1989). Superoxide dismutase: An adaptation to a pragmatic gas. Journal of Biological Chemistry, 264914), 7761 – 7764.
Goldberg, D., & Spooner, R. (1983). Assay of glutathione reductase In: Bergmeyer H., Bergmeyer J. and Grabl M., editors. Methods of Enzymatic Analysis.
Joshua, P. E., Ezugwu, C. H., Chilaka, F. C., Nwodo, O.F.C., Dasofunjo, K. and Ezuwu, M. U. (2018). Effect of Ethanol extract of Zapotecaportoricensis stem on testosterone-induced benign prostate hyperplasia (BPH) in adult male albino rats. Australian Journal of Basic and Applied Science, 12 (12), 9 – 18.
Joseph, N., Zhang-James, Y., Perl, A., &Faraone, S. V. (2015). Oxidative stress and ADHD: a meta-analysis. Journal of Attention Disorders, 19(11), 915-924.
Lim, K. B. (2017). Epidemiology of clinical benign prostatic hyperplasia. Asian Journal of Urology, 4(3), 148 – 151.
Liu, J., Fang, T., Li, M., Song, Y., Li, J., Xue, Z., Li, J., Bu, D., Liu, W and Zeng Q. (2019). Pao Pereira extract attenuates testosterone induced benign prostatic hyperplasia in rats by inhibiting 5α-reductase. Scientific Report, 9(1), 19703 – 19711.
Mbaka, G.O., Ogbonnia, S.O., Olarewaju, O. T. and Duru, F. L. (2013). The Effects of Ethanol seed extract of Raphia hookeri(palmaceae) on exogenous testosterone and estradiol induced benign prostatic hyperplasia in adult male rats. Journal of Morphological Science, 30(4), 235-243.
Minutoli, L., Rinaldi, M. and Marini, H. (2016). Apoptic pathways linked to Endocrine system as potential Therapeutic Targets for Benign Prostatic Hyperplasia. International Journal of Molecular Sciences, 17(8), 1311.
Nunes, R. V., Manzano, J., Truzzi, J. C., Nard, A., Silvinato, A. and Bernardo, W. M. (2017). Treatment of Benign Prostatic hyperplasia. Revista da Associacao Medica Brasileira, 63(2), 95 – 99.
Roehrborn, C.G., Nuckolls, J. G., Wei, J. T. and Steers, W. (2007). The benign prostatic hyperplasia registry and patient survey: Study design, methods and patient baseline characteristics. British Journal of Urology International, 100(4), 813-819.
Singh, R. K., Verma, P. K., Kumar, A., Kumar, S., Singh, R. P. and Acharya, A. (2020). Therapeutic Application of Carica papaya leaf extract in the management of human diseases. DARU Journal of Pharmaceutical Sciences, 28(2), 735 – 744.
Teixeria de silva, T. J., Zarse, K., Voigt, A., Urban, N., Birringer, M. and Ristow, M. (2014). Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress. Journal of Cell Metabolism, 6:280 – 293.
Traish, A. M. and Morgentalar, A. (2013). Effect of Finasteride on serum levels of androgenedione, testosterone and their 5 α-reductase metabolites in men at risk for prostate cancer. Journal of Steroid Biochemistry and Molecular Biology, 138, 462 – 463.
Wallin, B., Rosengren, B., Shertzer, H. G. and Camejo, G. (1993). Lipoprotein Oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtiter plate: it’s use for evaluation of Antioxidants. Analytical Biochemistry, 208(1), 10 – 15.
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Copyright (c) 2022 Lilian Ebenyi, Mathias Ominyi, Chidinma Anyanwu, Onwuchekwa Ogah, Moses Ogbanshi
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