Upregulation of Bdnf-Trkb and Mapk1/Erk Gene Expressions Improve Skeletal Muscle Strength in High Fat Diet-Fed Swiss Mice
Keywords:
High fat diet, Cannabidiol, Omega-3Abstract
Persistent consumption of foods rich in fats, sugars and calories have been implicated in a number of diseases including those of the nervous systems. This study investigated the role of co-administration of cannabidiol (CBD) and omega-3 in mice fed on high fat diet (HFD) for a period of 12 weeks. The animals were grouped as follows: Control (water and feed), HFD fed, HFD+CBD, HFD+omega-3, HFD+CBD+omega-3 and Omega-3 for 2 weeks post-HFD. They were subjected to wire hanging testing after the interventions. In the end, the animals fed HFD alone, had a statistically significantly shorter time hanging on the wire (p<0.05), when compared to the control, CBD and Omega treated animals. The amyloid deposits in the brains of the HFD-fed mice, after congo staining, was also significantly higher. The combination of CBD and omega-3 significantly cleared the brain of amyloid plaques, which is a pathological protein, that causes neuromuscular damage. qPCR gene expressions, for BDNF and MAPK, were significantly higher in the CBD plus omega-3 treated group compared to the HFD-fed group. This benefit was more pronounced in the post HFD group which had omega-3 for two weeks. Co-administration of CBD and Omega-3, in HFD-induced neuromuscular injury, is highly ameliorative. They are noted to increase endurance levels, during strenuous muscular exercise in mice. This is due to the clearance of amyloid proteins from the brain and upregulation of BDNF and MAPK genes. Also, stoppage of the HFD and administering omega-3 is of immense benefit.
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Copyright (c) 2026 Innocent Abi, Olasupo S. Adeniyi, Mustapha U. Imam (Author)
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