The Effect of Concurrent Administration of Cadmium and Arsenic through the Food Chain on some Testicular Toxicity Indicators of Rats
Keywords:
BAX, BCL, CADMIUM, ARSENIC, TESTESAbstract
This study examined the effect of concurrent administration of cadmium and arsenic through the food chain on testicular antioxidant status, protein concentration and expression of mRNA of Bax and Bcl-2 genes in rats. Methods: Catfish exposed to both metals at a concentration of 0.4 mg/metal/100mL for 1 month served as source of protein for the experimental diet which rats were exposed for 1 and 3 months. The metal burden on the feed and testes, activities of antioxidant enzymes, mRNA expression of Bax and Bcl-2 in the testes of rats were then determined using standard procedures. Result: The activities of the antioxidants enzymes and reduced glutathione (GSH) were significantly (p<0.05) decreased after 3 months. Testes total protein and MDA levels were significantly (p< 0.05) increased after both periods of exposure. Increase in the level of mRNA expression of Bax gene and a decrease in Bcl-2 gene in the test groups compared to the control after 3 months of exposure were recorded in rats. Conclusion: These results showed exposure to these metals through the food chain increased oxidative damage leading to alteration in the expression of Bax/Bcl-2 ratio which could have significant consequences in the induction of apoptosis and other associated biochemical cascade.
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Anyanwu, B. O., Ezejiofor, A. N., Igweze, Z. N. and Orisakwe, O. E. (2018). Heavy metal mixture exposure and effects in developing nations: An update. Toxics, 6(4), 65, doi:10.3390/toxics6040065
Akinboro, A., Adedosu, O. T., Badmus, J. A., Adegbola, P. I., Abubakar, M. Y., James, R.O. and Aborisade, A. B. (2022). Aspalathus Linearis extract ameliorate haematological disorder, dyslipidaemia and tissue toxicity associated with arsenic exposure in rats. Phytomedicine Plus, 2(1), 100171.
Beutler, E., Duron, O. and Kelly, B. M. (1963). Improved method for determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888.
Casalino, E., Calzaretti, G., Sblano, C. and Landriscina, C. (2002). Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicology, 179(1-2), 37–50.
Chaudhari, R. T., Harshad, R. K. and Thorat, S. R. (2015). Effect of cadmium chloride on the biochemical charecteristics of fresh water fish, Cyprinus carpio. International Journal of Management and Social Science, 3(3), 85-89.
Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidiniumthiocyanate–phenol–chloroform extraction. Analytical Biochemistry, 162(1), 156-159.
Choudhury, H. and Mudipalli, A. (2008). Potential considerations and concerns in the risk characterization for the interaction profiles of metals. Indian Journal of Medical Research, 128(4), 462-483.
Christian, E. I., Olatoye, T. R., Bamidele, F. S., Ojo, O. E. and Ademoye, K. A. (2016). Cadmium-induced testicular toxicity, oxidative stress and histopathology in Wistar rats: sustained effects of polyphenol-rich extract of Vernonia amygdalina leaf. Journal of Interdisciplinary Histopathology, 4(3), 54-62.
Dooley, P. A., Tsarouhtsis, D., Korbel, G. A., Nechev, L. V., Shearer, J., Zegar, I. S., Harris, C. M., Stone, M. P. and Harris, T. M. (2001). Structural studies of an oligodeoxynucleotide containing a trimethylene interstrand cross-link in a 5'-(CpG) motif: model of a malondialdehyde cross-link. Journal of the American Chemical Society, 123(8), 1730-1739.
Ezedom, T., Asagba, S. and Tonukari, N. J. (2020). Toxicological effects of the concurrent administration of cadmium and arsenic through the food chain on the liver and kidney of rats. Journal of Basic and Applied Zoology, 81(1), 1-12.
Ezedom, T. and Asagba, S.O. (2016). Effect of a controlled food-chain mediated exposure to cadmium and arsenic on oxidative enzymes in the tissues of rats. Toxicology Reports, 3, 708–715.
Giaccio, L., Cicchella, D. De Vivo, B., Lombardi, G. and De Rosa, M. (2012). Does heavy metals pollution affects semen quality in men? A case of study in the metropolitan area of Naples (Italy). Journal of Geochemical Exploration, 112, 218–225.
Gornall, A., Bardsmill, C. T. and David, M. M. (1949). Determination of serum protein by means of biuret reaction. Journal of Biological Chemistry, 177(2), 751-766.
Gutteridge, J. M. C. and Wilkins, C. (1982). Copper dependent hydroxyl radical damage to ascorbic acid. Formation of a thiobarbituric acid-reactive product. FEBS Letters, 137(2), 327-340.
Habig, W. H., Pabst, M.J. and Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249 (22), 7130-7139.
Hata, A. N., Engelman, J.A. and Faber, A. C. (2015). The BCL2 Family: Key mediators of the apoptotic response to targeted anticancer therapeutics. Cancer Discovery, 5(5), 475–87.
Horiguchi, H., Sato, M., Konno, N. and Fukushima, M. (1996). Long-term cadmium exposure induces anemia in rats through hypoinduction of erythropoietin in the kidneys. Archives of Toxicology, 71, 11–9.
Khan, M.A., Khan, S., Khan, A. and Alam, M. (2017). Soil contamination with cadmium, consequences and remediation using organic amendments. The Science of the Total Environment, 601–602: 1591–1605.
Kumar, K. M. K., Kumar, N. M., Nagesh, R., Patil, R. H., Babu, R. L., Sharma, S.C. and Manjunath, K. (2017). Arsenic induces oxidative stress and apoptosis in human lung cancer cells. International Journal of Life Sciences, 5 (4): 551-560.
Lehrach, H., Diamond, D., Wozney, J. M. and Boedtker, H. (1977). RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical re-examination. Biochemistry, 16(21), 4743–51.
Leibowitz, B. and Yu J. (2010). Mitochondrial signaling in cell death via the Bcl-2 family. Cancer Biology and Therapy, 9(6):417–22.
Mahaffey, K. R., Capar, S. G., Gladen, B. C. and Fowler, B. A. (1981). Concurrent exposure to lead, cadmium, and arsenic. Effects on toxicity and tissue metal concentrations in the rat. Journal of Laboratory and Clinical Medicine, 98(4), 463-81.
Mandal, P. (2017). An insight of environmental contamination of arsenic on animal health. Emerging Contaminants, 3(10), 17-22.
Misra, H. P., and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of Biological Chemistry, 247(10), 3170–3175.
Moazamian, R., Polhemus, A., Connaughton, H., Fraser, B., Whiting, S., Gharagozloo, P. and Aitken, R. J. (2015). Oxidative stress and human spermatozoa: diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation. Molecular Human Reproduction, 21(6), 502–515.
Nachmias, B., Ashhab, Y. and Ben-Yehuda, D. (2004). The inhibitor of apoptosis protein family (IAPs): an emerging therapeutic target in cancer. Seminars in Cancer Biology, 14(4), 231–43.
Naseri, M. H., Mahdavi, M., Davoodi, J., Tackallou, S. H., Goudarzvand, M., & Neishabouri, S. H. (2015). Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells. Cancer Cell International, 15(1).doi:10.1186/s12935-015-0204-2.
Nna, V. U., Bakar, A. B. A., Ahmad, A., Eleazu, C. O. and Mohamed, M. (2019). Oxidative stress, NF-κB-mediated inflammation and apoptosis in the testes of streptozotocin–induced diabetic rats: combined protective effects of malaysian propolis and metformin. Antioxidants, 8(10), 465.
Pavlova, E. and Atanassova, N. (2018). Impact of cadmium on male fertility. Acta Morphologica et Anthropologica, 25(1-2), 108-116.
Rana, S. V. S. (2018). Protection of metal toxicity by melatonin-recent advances. EC Pharmacology and Toxicology, 6.9, 851-864.
Schmolke, G., Elsenhans, B., Ehtechami, C. and Forth, W. (1992). Arsenic-copper interaction in the kidney of the rat. EC Pharmacology and Toxicology, 11(5), 315-21.
Sexton, K. and Hattis, D. (2007). Assessing cumulative health risks from exposure to environmental mixtures - three fundamental questions. Environmental health perspectives, 115(5), 825–832. https://doi.org/10.1289/ehp.9333
Sinha, A. K. (1972). Colorimetric assay of catalase. Analytical Biochemistry, 47(2), 389-394.
Sukhn, C., Awwad, J., Ghantous, A. and Zaatari, G. (2018). Associations of semen quality with non-essential heavy metals in blood and seminal fluid: data from the environment and male infertility (EMI) study in Lebanon. Journal of Assisted Reproduction and Genetics, 35:1691–1701.
Zhang, F. Q., Wang, Y. S., Lou, Z. P. and Dong, J. D. (2007). Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere, 67(1), 44-50
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