Assessments of the Safety of Arsenicals on Dyslipidaemia and Reproductive Organ Morphology of Albino Rats
DOI:
https://doi.org/10.4314/njbmb.v39i2.3Keywords:
Arsenic, lipid profile, sub-chronic exposure, gender, histology, lipoproteinsAbstract
Epidemiological studies have implicated Arsenic (As) an environmental toxicant in the etiology of many diseases which have been associated with dyslipidaemia and cardiovascular abnormalities. This study investigated the comparative effect of sub-chronic exposure to two arsenicals on the lipid profiles and morphology of the reproductive organs. Fifty albino rats were divided into five groups of 10 animals each (5 male and 5 female) were exposed to different doses of arsenic (As) either as sodium arsenite or sodium arsenate for 5 weeks. Lipids [triacylglycerol (TAG), cholesterol (CHOL) and phospholipids (PHOS)] concentrations were determined in the plasma, lipoprotein fractions, hepatic, renal, cardiac and brain tissues. In the male rats’ tissues, both arsenicals generally elicited a hormetic response, while in the tissues of female rats; both arsenicals increased the CHOL concentration. Furthermore, the perturbations in TAG concentration in female animals did not follow any regular pattern; although, depletion of TAG characterized these arsenic-induced perturbations in male rats except in the kidney, where TAG was accumulated. The arsenicals generally increased PHOS concentration in exposed animals irrespective of the sex. While HDL-TAG and HDL-CHOL concentrations were significantly reduced in As-exposed groups, changes observed in VLDL+LDL-TAG and VLDL+LDL-CHOL varied with no regular pattern. Histopathology of the sex organs revealed altered morphology in arsenite-exposed rats. Results from this study further associated these arsenicals as potential agents that can cause dyslipidaemia in tissues and also possess the ability to alter the architecture of sex organs in albino rats.
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Adeyi, O. E. (2021). Arsenic-induced perturbations in cellular respiration in male albino rats. A Ph.D. Thesis submitted to the Department of Biochemistry, Federal University of Agriculture, Abeokuta. Nigeria.
Adeyi, O. E., Babayemi, D. O., Ajayi, B. O., Adeyi, A. O., Ayodeji, A. H., Oguntayo, A. O., Adeyemi, A. T., Olaiyapo, O. E. and Adeoye, S. T. (2021). Co-administration of sodium selenite and sodium arsenite exacerbates hepatic, renal, pulmonary and splenic inflammation in rats. Scientific African, 11(2021), e00708.
Afolabi, O. K., Ugbaja, R. N., Arinola, A., Olorunnisola, O. S., and Adeleke, G. E. (2016). Catechin protects against dyslipidemia and nephro-hepatototoxicity induced in rats exposed to arsenic. Jordan Journal of Biological Sciences, 9, 173-177.
Afolabi, O. K., Wusu, A. D., Ogunrinola, O. O., Abam, E. O., Babayemi, D. O., Dosumu, O. A., Onunkwor, O. B., Balogun, E. A., Odukoya, O. O. and Ademuyiwa, O. (2015). Arsenic-induced dyslipidemia in male albino rats: comparison between trivalent and pentavalent inorganic arsenic in drinking water. BMC Pharmacology and Toxicology, 16, 1-15.
Chi, L., Lai, Y., Tu, P., Liu, C. W., Xue, J., Ru, H. and Lu, K. (2019). Lipid and cholesterol homeostasis after arsenic exposure and antibiotic treatment in mice: Potential role of the microbiota. Environmental Health Perspective, 12, 097002.
Cohen, S. M., Arnold, L. L., Eldan, M., Lewis, A. S. and Beck, B. D. (2006). Methylated arsenicals: the implications of metabolism and carcinogenicity studies in rodents to human risk assessment. Critical Reviews in Toxicology, 36, 99 –133.
Cypriani, B. Tabacik, C. and Descomps, B. (1988). Effect of estradiol and antiestrogens on cholesterol biosynthesis in hormone-dependent and independent breast cancer cell lines. Biochimica et Biophysica Acta, 972, 167-178.
De Marinis, E., Martini, C., Trentalance, A. and Pallottini, V. (2008). Sex differences in hepatic regulation of cholesterol homeostasis. Journal of Endocrinology, 198, 635-643.
Ellen, T. P. and Costa, M. (2010). 14.08–Carcinogenic inorganic chemicals A2-McQueen, Charlene A. In: Comprehensive Toxicology, Elsevier Oxford. pp. 139-160.
Folch, J., Lees, M. and Sloane, S. G. H (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509.
Hanukoglu, I. (1992). Steroidogenic enzymes: structure, function, and role in regulation of steroid hormone biosynthesis. The Journal of Steroid Biochemistry and Molecular Biology, 43, 779-804.
Hui, S., Ghergurovich, J. M., Morscher, R. J., Jang, C., Teng, X., Lu, W., Esparza, L. A., Reya, T., Zhan, L. and Guo, J.Y. (2017). Glucose feeds the TCA cycle via circulating lactate. Nature, 551, 115–118.
Jansen, R. J., Argos, M., Tong, L., Li, J., Rakibuz-Zaman, M., Islam, M. T., Slavkovich, V., Ahmed, A., Navas-Acien, A., Parvez, F., Chen, Y., Gamble, M. V., Graziano, J. H., Pierce, B. L. and Ahsan, H. (2016). Determinants and consequences of arsenic metabolism efficiency among 4,794 individuals: Demographics, lifestyle, genetics, and toxicity. Cancer Epidemiology Biomarkers Prevention, 25, 381–90.
Kang, H. M., Ahn, S. H., Choi, P., Ko, Y. A., Han, S. H., Chinga, F., Park, A. S. D., Tao, J., Sharma, K. and Bottinger, E. P. (2015). Defective fatty acid oxidation in renal tubular epithelial cells has a key role in kidney fibrosis development. Nature Medicine, 21, 37-46.
Mao, Z., Li, J. and Zhang, W. (2018). Hormonal regulation of cholesterol homeostasis. Open access peer reviewed chapter. In: Intechopen. DOI: 10.5772/intechopen.76375
Masuda, H. (2018). Arsenic cycling in the Earth crust and hydrosphere: Interaction between naturally occurring arsenic and human activities. Progress in Earth and Planetary Science, 5, 68-79.
Maxwell, S. R. J. (1998). Women and heart disease. Basic Research in Cardiology, 93, 79–84.
Mennecozzi, M., Landesmann, B., Palosaari, T., Harris, G. and Whelan, M (2015). Sex differences in liver toxicity Do female and male human primary hepatocytes react differently to toxicants in vitro? PLoS ONE, 10(4), e0122786
Muhetaer, M., Yang,, M., Xia, R., Lai, Y. and Wu, J. (2022). Gender difference in arsenic biotransformation is an important metabolic basis for arsenic toxicity. BMC Pharmacology and Toxicology, 23,15-28.
Nelson, D. L. and Cox, M. M. (2009). Lehninger principles of biochemistry. New York: WH Freeman.
Nicolson, T. J., Mellor, H. R. and Roberts, R. A. (2010). Gender differences in drug toxicity. Trends in Pharmacological Sciences, 31 (3), 108-114.
Qamar, A and Bhatt, D. L. (2015). Effect of low cholesterol on steroid hormones and vitamin E levels. Circulation Research, 117, 662-664.
Rai, A., Maurya, S., Khare, P., Srivastava A. and Bandyopadhyay, S. (2010). Characterization of developmental neurotoxicity of As, Cd, and Pb mixture: synergistic action of metal mixture in glial and neuronal functions. Toxicological Sciences, 118, 586–601.
Sarkar, D. and Rupali, D. (2007). Biogeochemistry of arsenic in contaminated soils of superfund sites. EPA. United States Environmental Protection Agency.
Scerbo, D., Son, N. H., Sirwi, A., Zeng, L., Sas, K. M., Cifarelli, V., Schoiswohl, G., Huggins, L. A., Gumaste, N., Hu, Y. and Pennathur, S. (2017). Kidney triglyceride accumulation in the fasted mouse is dependent upon serum free fatty acids. Journal of Lipid Research, 58, 1132-1142.
Singh, M. K., Singh, P. K., Yadav, S. S., Singh, U. S., Dwivedi, P. and Yadav, R. S. (2018). Attenuation of arsenic-induced dyslipidemia by fruit extract of Emblica officinalis in mice. International Journal of Nutrition, Pharmacology, Neurological Diseases, 8, 1-9.
Smith, A. H. and Steinmaus, C. M. (2011). Arsenic in drinking water. British Medical Journal, 342: d2248–d2248. doi:10.1136/bmj.d2248.
Steinberg, D. (2006). The Pathogenesis of atherosclerosis. An interpretive history of the cholesterol controversy, Part IV: The 1984 Coronary primary prevention trial ends it-almost. Journal of Lipid Research, 47, 1-14.
Stewart, J. C. M. (1980). Colourimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry, 104, 10-14.
Ugbaja, R. N., Akinloye, D. I., Akamo, A. J., Ugwor, E. I., IIesanmi, O. O., Tytler, O., Ogunbona, M. and Ademuyiwa, O. (2016). Ameliorative effects of Aloe vera gel extract on circulatory and tissue lipid profile status in streptozotocin-induced diabetic rats. Bangladesh Journal of Medical Biochemistry, 9, 11-26.
Wang, C. H., Hsiao, C. K., Chen, C. L., Hsu, L. I., Chiou, H. Y., Chen, S. Y., Hsueh, Y. M., Wu, M. M. and Chen, C. J. (2007). A review of the epidemiologic literature on the role of environmental arsenic exposure and cardiovascular
diseases. Toxicology Applied Pharmacology, 222, 315-326.
Wengrofsky, P., Lee, J. and Makaryus, A. N. (2019). Dyslipidemia and its role in the pathogenesis of atherosclerotic cardiovascular disease: implications for evaluation and targets for treatment of dyslipidemia based on recent guidelines. In Dyslipidemia. IntechOpen. Doi:10.5772/intechopen.85772.
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Copyright (c) 2024 Olubisi E. Adeyi, Oluwatobi T. Somade, Emmanuel I. Ugwor, Olayinka A. Oladimeji, Happiness O. Ozoemena, Akindele O. Adeyi (Author)
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