Evaluation of Advanced Glycation End Products and DPPH (1-diphenyl-2-picryl-hydrazyl) Inhibitory Potential of Solanum macrocarpon Fruits and Leaves: An In vitro Study
Keywords:
Antiglycation, Free radical, Phytochemicals, Solanum macrocarponAbstract
Advanced Glycation End Products (AGEs), are produced when sugars and free amino groups in proteins undergo a non-enzymatic event known as glycation. A key therapeutic strategy against the advancement of glycation and its associated health issues, such as hyperglycemia and atherosclerosis, is the inhibition of AGE generation. The present study was carried out to evaluate the qualitative phytochemical constituent as well as in vitro antioxidant and antiglycation potential of extracts from fruit and leaves of Solanum macrocarpon. AGEs derived from incubation of bovine serum albumin (BSA) and glucose was characterized by spectrofluorescence. Fruit and leaf samples were extracted with chloroform, ethyl acetate and methanol, followed by phytochemical constituent evaluation, antioxidant and antiglycation determination. Our results indicated the presence of alkaloids, saponins, tannins and flavonoids in all the plant extracts. Results obtained from the antioxidant assay showed highest percentage antioxidant activity for both the fruit and leaf extracts of S. macrocarpon were found in the methanolic extract (98.95%) and chloroform extract (92.09%) respectively. Similarly, the highest percentage antiglycation activity for both fruit and leaf extracts were found in methanolic extract (71.84%) and ethyl acetate extract (62.91%) respectively. The plant thus, exhibit the ability to significantly decrease 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical levels, alongside advanced glycation end products (AGES), implying its potential as an ameliorating agent.
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