Effects of Black Seed (Nigella Sativa) Oil on Hepatic Function Parameters in Rats Induced with Diabetes Mellitus and Periodontitis
DOI:
https://doi.org/10.4314/njbmb.v39i3.7Keywords:
Diabetes, Periodontitis, Liver, Nigella sativaAbstract
This study evaluated the effect of Nigella sativa (NS) seed oil on hepatic function in rat model of periodontitis and diabetes mellitus. Forty-eight Wistar rats were divided into eight groups of six rats each as follows: Group I rats were fed with normal rat chow ad libitum without any induction and served as Control. Group II rats were fed with normal rat chow, water and administered NS oil. Group III rats were induced with diabetes without treatment. Group IV rats were treated with 1 ml/kg bwt NS oil intraperitoneally after diabetes induction (DB + NS.). Group V were induced with periodontitis without treatment. Group VI rats were treated with 1 ml/kg bwt NS oil intraperitoneally after periodontitis induction. Group VII were induced with diabetes and periodontitis without treatment and finally Group VIII rats were treated with 1 ml/kg bwt NS oil after diabetes and periodontitis induction (DB+PD+NS) intraperitoneally. The result showed that treatment with Nigella sativa oil significantly reduced Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) activities in Diabetes mellitus and periodontitis group compared with untreated groups. In addition, diabetes and periodontitis disrupted the normal architecture of the liver in the untreated group while the oil ameliorated these effects significantly in the treatment groups. The histology of the jaw showed varying degrees of inflammation in the untreated diabetes and periodontitis group, but these effects were reduced in the treated groups. In conclusion, black seed oil ameliorated the effect of diabetes mellitus and periodontitis in the liver of the Diabetes mellitus and periodontitis induced rats.
Downloads
References
Abd-Elkareem, M., Soliman, M, Abd El-Rahman, M. A. M. and Abou Khalil NS (2022) Effect of Nigella sativa l. Seed on the kidney of monosodium glutamate challenged rats. Frontiers in. Pharmacology, 13:789988. doi: 10.3389/fphar.2022.789988
Adewole, S. O., Caxton-Martins, E. A. and Ojewole, J. A. O. (2006). Histochemical and biochemical effects of melatonin on pancreatic-cells on streptozocin –treated diabetic rats. Pharmacology-Online, 2: 1-21
Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A. and Anwar, F (2013). A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3, 337–352.
Ahmad, M.F., Ahmad, F.A., Ashraf, S.A., Saad, H.H., Wahab, S., Khan, M.I., Ali, M., Mohan, S., Hakeem, K.R. and Athar, M.T. (2020). An updated knowledge of black seed (Nigella sativa Linn): Review of phytochemical constituents and pharmacological properties. Journal of Herb Medicine, 2: 100-110.
Akhtar, M. T., Qadir, R. Bukhari, I., Ashraf1, R. A, Zahoor, Z. M., Murtaza, M. A., Siddique, F., Shah, S. N. H. and Saadia, M. (2020). Antidiabetic potential of Nigella sativa L seed oil in alloxan-induced diabetic rabbits. Tropical Journal of Pharmaceutical Research; 19 (2): 283-289.
Al-Bayaty F., Kamaruddin A., Ismail M. and Abdulla M. (2013). Formulation and Evaluation of a New Biodegradable Periodontal Chip Containing Thymoquinone in a Chitosan Base for the Management of Chronic Periodontitis. Journal of Nanomaterials, 39(7):308-320.
Alimohammadi, S, Hobbenaghi, R. and Javanbakht, J. (2013). Protective and antidiabetic effect of extract from Nigella sativa on blood glucose concentrations against streptoztocin (STZ)-induced diabetic rats: an experimental study with histopathological evaluation. Diagnostic Pathology, 8:137-143.
Al-Logmani, A. and Zari, T. (2011). Long-term effects of Nigella sativa L. oil on some physiological parameters in normal and streptozotocin-induced diabetic rats. Journal of Diabetes Mellitus, 1: 46-53.
Al-Wafi, H. and Shaker A., (2014). Benefits of thymoquinone, a Nigella Sativa extract in preventing dental caries initiation and improving gingival health. ProQuest LLC 2014: 72.
Asgary S., Sahebkar A. and Goli-Malekabadi N. (2015). Ameliorative effects of Nigella sativa on dyslipidemia. Journal of Endocrinology Investigation, 38(10):1039-46.
Bancroft, S. L., Bancroft, J. and Gamble, M., (2013). Tissue processing. Bancroft's Theory and Practice of Histological Techniques. 7nd ed. Netherlands, Amsterdam: Elsevier Health Sciences, 105-123.
Baragob, A. E. A, (2015). Composition and hypoglycemic effect of CM in streptozotocin- diabetic rats. Biochemistry and Biotechnology Research; 3:38-42.
Belgaumi, U.I., Patil, S. and Gandhi, J. M. (2020). The many therapeutic applications of Nigella sativa- a review of literature. Journal of Evolution Medicine and Dental Science, 9(30):2151-2157.
Bilgic, S., Tastemir, D. K., Azirak, S., Guvenc A. N., Kocaman, N. and Ozer M. K. (2017). The protective effect of thymoquinone over olanzapine-induced side effects in liver, and metabolic side effects. Bratisl Lek Listy, 118(10): 618-625.
Bujanda, L., Hijona, E. and Larzabal, M. (2008). Resveratol inhibits nonalcoholic fatty liver disease in rats. BMC Gastroentrology, 8:40-8.
Busari, A. A., Orororo, O. C., Opute, U. A., Efejene, I. O., Badmos, K., Obadiah, C. C., Akinshipo, W. A. and Egbune, E. O. (2023). Effects of black seed oil on oxidative stress parameters and gingival expression of inducible nitric oxide synthase in diabetes and periodontitis-induced rats. African Scientist, 24(3): 376-386.
Carvalho, J.S., Di Lenardo, D., Alves, E.H.P., Carvalho, A.S., Silva, F.R.P., França, L.F.C., Nóbrega Neto, A.P.R., Vasconcelos, A.C.C.G., Souza, L.K.M., Barbosa, A.L.R., Medeiros J.V.R., Oliveira, J.S. and Vasconcelos, D.F.P. (2016) Steatosis caused by experimental periodontitis is reversible after removal of ligature in rats. Journal of Periodontal Research; 8(3):13-25.
Chavarry, N.G.M., Vettore, M.V., Sansone C. and Sheiham, A (2009). The relationship between diabetes mellitus and destructive periodontal disease: A meta-analysis. Oral Health and Preventive Dental, 7:107–127
Cheng, D., Liang B. and Li, Y. (2013). Antihyperglycemic effect of Ginkgo biloba extract in streptozotocin-induced diabetes in rats. Biomedical Research International, 6:1-7.
Ekakitie, L. I., Okpoghono, J., Orororo O. C. and Ekakitie, O. A. (2021) Ameliorative prowess of bee honey in the tissues of rats administered aluminium nitrate. Scientific African; 12 (2021) e00782:1-9
Elizabeth, H. and Harris, M. D. (2005). Elevated liver function tests in type 2 diabetes. Clinical Diabetes, 23: 115-11
Hadi, S., Mirmiran, P. and Hadi, V, (2016). Effects of Nigella sativa on cardiovascular risk factors. Journal of Mazandaran University of Medical Science, 25(134):430-442.
Hamad, E. M., Abdel-Rahim, E. A. and Romeih, E. A. (2011). Beneficial effect of CM on liver and kidneys function in diabetic Sprague-Dawley rats. International Journal of Dairy Science, 6:190-7.
Hamdan, A. Idrus R. H. and Mokhtar M. H. (2019). Effects of Nigella sativa on type-2 diabetes mellitus: A systematic review. International Journal of Environmental Research and Public Health, 16: 49-61.
Hatipoglu, M., Alptekin, N.Ö., Avunduk, M.C. (2016). Effects of alpha-tocopherol on gingival expression of inducible nitric oxide synthase in the rats with experimental periodontitis and diabetes. Nigerian Journal of Clinical Practice, 19:480-5. 2016.
Hung, H. Y., Qian, K., Morris-Natschke, S.L., Hsu, C.S. and Lee, K. H. (2012). Recent discovery of plant-derived anti-diabetic natural products. National Production Report; 29(5): 580-606.
Ilangovan, S. and Rajasekar, A. (2021). Evaluation of Efficacy of 20% Nigella sativa on Gingival Health. Journal of Pharmaceutical Research International; 33(61A): 354-363,
International Diabetes Federation (IDF) (2017). Diabetes atlas.8th ed. Brussels: International Diabetes Federation; 2017.
Kassab, R.B. and El-Hennamy, R.E. (2017). The role of thymoquinone as a potent antioxidant in ameliorating the neurotoxic effect of sodium arsenate in female rat. Egyptian Journal of Basic and Applied Sciences, 4, 160–167.
Keith, G. and Tolman, M.D. (2004). Hepatotoxicity of non-narcotic analgesics. The American Journal of Medicine; 105(1): S213-S219.
Keith G., Tolman M.D., Fonseca, A.V., Meng, H. and Dalpiaz, A. (2004). Narrative review hepatobiliary disease in type 2 diabetes mellitus. Animals of internal Medicine; 12, 141, 946 – 952.
Kemink, S.A.G., Hermus A.R.M.M., Swinkels L.M.J.W, Lutterman J.A. and Smals, A.G.H (2000). Osteopenia in insulin-dependent diabetes mellitus; prevalence and aspects of pathophysiology. Journal of Endocrinology Investigation, 23: 295-303.
Kiari, F. Z., Meddah, B. and Meddah, A. T. T. (2018) In vitro study on the activity of essential oil and methanolic extract from Algerian Nigella sativa L. Seeds on the growth kinetics of micro-organisms isolated from the buccal cavities of periodontal patients. Saudi Dental Journal; 30, 312–323.
Madianos, P. N., Bobetsis, Y. A. and Kinane, D. F. (2005). Generation of inflammatory stimuli: How bacteria set up inflammatory responses in the gingiva. Journal of Clinical Periodontology, 32:57-71.
Mahmoud, Y.K. and Abdelrazek, H.M.A. (2019). Cancer: Thymoquinone antioxidant/pro-oxidant effect as potential anticancer remedy. Biomedical Pharmacotherapy, 115, 108783.
Maideen, N.M.P. (2020). Prophetic Medicine-Nigella Sativa (Black cumin seeds) - Potential herb for COVID-19? Journal of Pharmacopuncture, 23, 62–70.
Mansour, M.A., Nagi, M.N. and El-Khatib, A.S, (2002). Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: A possible mechanism of action. Cell Biochemical Functions, 20:143–151.
Marchesan, J.T., Girnary, M.S., Moss, K.; Monaghan, E.T., Egnatz, G.J., Jiao, Y.; Zhang, S.;,Beck, J. and Swanson, K.V. (2020). Role of inflammasomes in the pathogenesis of periodontal disease and therapeutics. Periodontology, 82, 93–114.
Mazumdar, M., Chatterjee, A., Majumdar, S., Chandrika, M. and Patki, P. S. (2013). Evaluation of the safety and efficacy of complete care herbal toothpaste in controlling dental plaque, gingival bleeding and periodontal diseases. Journal of Homeop Ayurv Medicine, 2: 124.
Mealey, B. L. and Ocampo, G. L. (2007). Diabetes mellitus and periodontal disease. Periodontology; 44:127–153.
Mehana, E. E., Meki, A. R. and Fazili, K. M. (2012). Ameliorated effects of green tea extract on lead induced liver toxicity in rats. Expert Toxicology and Pathology, 64(4): 291-295
Mekhemar M., Hassan Y. and Dörfer C. (2020). Nigella sativa and thymoquinone: A natural blessing for periodontal therapy. Antioxidants, 9:12-30
Meyer S.A. and Kulkarni A.P. (2001). Hepatotoxicity. In: Introduction to biochemical toxicology. 3rd Edn. Newyok: John Wiley and Sons, p487.
Mohamed, E. (2010). Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Journal of Pathology, 200:647–655.
Mohebbati, R. and Abbasnezhad, A. (2020). Effects of Nigella sativa on endothelial dysfunction in diabetes mellitus: A review. Journal of Ethnopharmacology, 25 (2):112-120.
Morakinyo, A. O., Adekunbi, D. A., Dada, K. A. and Adegoke, O. A. (2014). Testosterone promotes glucose intolerance, lipid disorder and oxidative stress in type 1 diabetic rats. Journal of Basic Clinical Physiolology and Pharmacology, 25:13 20
Olfert, E. D., Cross, B. M. and McWilliams, A. A. (1993) Guide to the care and use of experimental animals. 2nd ed. Ottawa, Canada: Canadian Council on Animal Care; p. 82-93.
Orororo, O. C., Asagba, S. O., Oghri, E. and Egbune Egoamaka Oliseneku (2017) Comparative effect of garden egg, carrot and oat on biochemical parameters in cadmium exposed rats. African Journal of Biochemistry Research, 12(3): 28-34.
Orororo, O. C., Efekemo, O. and Udi, O. A. (2022). Changes in liver histology, hematological parameters and lipid profile of cadmium-exposed rats treated with combined leaf extract of Vernonia amygdalina and Occimum gratissimum. Asian Journal of Medicine and Health; 20(11):195-203
Orororo, O. C., Mordi, J. C., Opute, U. A., Efejene, I. O., Egbune, E. O., Busari, A. A., Badmos, K., Obadiah, C. C. and Akinshipo, W. A. (2023). Black seed oil-induced amelioration of renal dysfunction in a rat model of diabetes mellitus and periodontitis. Tropical Journal of Natural Product Research, 7(7):3524-3531.
Osawa T and Kato Y. (2005). Protective role of antioxidative food factors in oxidative stress caused by hyperglycemia. Annals of New York Academy of Science, 1043:440–451.
Ozdemir, H., Kara, M. I., Erciyas, K., Ozer, H. and Ay, S. (2012). Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. Journal of Periodontal Research, 47: 74-80.
Page, R. C. and Kornman, K. (2000). The pathogenesis of human periodontitis: An introduction. Periodontology, 14:9-11.
Papapanou, P.N., Sanz, M., Buduneli, N., Dietrich, T., Feres, M., Fine, D.H., Flemmig, T.F., Garcia, R., Giannobile, W.V. and Graziani, F. (2018). Periodontitis: Consensus report of
workgroup 2 of the 2017 world workshop on the classification of periodontal and peri-implant diseases and conditions. Journal of Periodontology, 89 (Suppl. S1): S173–S182.
Preshaw P.M., Alba L.A., Herrera D., Jepsen S., Konstantinidis A., Makrilakis K. and Taylor R. (2012). Periodontitis and diabetes: a two-way relationship. Diabetologia, 55:21–31.
Rajasekar, A. and Mathew, M. G. (2021). Prevalence of periodontal disease among individuals between 18-30 years of age: A retrospective study. Annals of Medical Health Science Research, 5:25-35.
Reitman, S. and Frankel, S. A. (1957). Colomentric method for determination of serum glutamic oxalacetic acid and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28: 56–63.
Salvi, G. E., Carollo-Bittel, B. and Lang, N. P. (2008). Effects of diabetes mellitus on periodontal and peri-implant conditions:Udate on associations and risks. Journal of Clinical Periodontology, 35:398–409.
Shaterzadeh-Yazdi, H., Noorbakhsh, M.F., Hayati, F., Samarghandian, S. and Farkhondeh, T. (2018). Immunomodulatory and anti-inflammatory effects of thymoquinone. Cardiovascular and Hematological Disorder Drug Targets, 18:52–60.
Shori, A.B, (2015). Camel milk as a potential therapy for controlling diabetes and its complications: A review of in vivo studies. Journal of Food and Drug Analysis, 23:609-618.
Silva, N., Abusleme, L., Bravo, D., Dutzan, N., Garcia-Sesnich, J., Vernal, R., Herna´ndez, M. and Gamonal, J. (2015). Host response mechanisms in periodontal diseases. Journal of Applied Oral Science, 23, 329–355.
Usman, M., Ali, M. Z., Qureshi, A. S., Ateeq, M. K. and Nisa, F. U. (2017). Short term effect of dose dependent camel milk in alloxan induced diabetes in female albino rats. Journal of Animal and Plant Science, 23 (3):25-38
Van Leeuwen, M. P., Slot, D. E., and Van der Weijden, G. A. (2011). Essential moils compared to chlorhexidine with respect to plaque and parameters of gingival inflammation: a systematic review. Journal of Periodontology, 82: 174-194.
Wahid, A., Chaudhry, S., Ehsan, A., Butt, S. and Khan, A. A. (2013). Bidirectional relationship between chronic kidney disease and periodontal disease. Pakistan Journal of Medical Science, 29: 211-215.
Yimer, E.M., Tuem, K.B., Karim, A., Ur-Rehman, N., Anwar, F. (2019). Nigella sativa L. (Black Cumin): A promising natural remedy for wide range of illnesses. Evidences Based Complementary and Alternative Medicine, 16:23-40
Additional Files
Published
Data Availability Statement
NA
Issue
Section
Categories
License
Copyright (c) 2025 Ugbome A. Opute, Osuvwe C. Orororo, Ofejiro I. Efejene, Abdulwasiu A. Busari, Kabir Badmos, Cynthia C. Obadiah, Warith A. Akinshipo, Ejiro P. Awhin, Othuke B. Odeghe, Edith O. Okoro, Egoamaka O. Egbune (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
