Comparative Evaluation of the Nutritional Composition and Antioxidant Activity of Roasted and Unroasted Date Seed (Phoenix dactylifera) Powder Extracts
Keywords:
Phoenix dactylifera, Roasting, Proximate compositionAbstract
The date seed (Phoenix dactylifera L.), a member of the Arecaceae family, produces seeds rich in dietary fiber, phenolics and essential nutrients. This study investigated the nutritional composition and antioxidant potential of roasted and unroasted Phoenix dactylifera seed powder extracts. Proximate, vitamin and mineral analyses were conducted using standardized methods. Roasting decreased moisture, and protein while carbohydrate and fat increased (carbohydrate: 73.92 ± 0.13 to 76.80 ± 0.06 %; fat: 5.54 ± 0.02 to 5.90 ± 0.04 %). while ash and crude fiber showed minor variations. Roasting reduced vitamins C, B1, B2, B3 and A. Mineral analysis showed high potassium in both samples, while roasted samples had more iron and zinc; with magnesium, sodium, calcium and phosphorus also well represented. Antioxidant activity by DPPH assay revealed an IC₅₀ of 26.91 ± 0.68 µg/ml (unroasted) and 44.97 ± 0.63 µg/mL (roasted), while standard ascorbic acid showed 1.62 ± 0.04 µg/ml. FRAP peaked at 93.60 ± 0.46 % (unroasted), 92.00 ± 0.28 % (roasted) and 98.40 ± 0.27 % (ascorbic acid). Unroasted seed powder retained more nutritional and antioxidant value, supporting its use in functional foods and nutraceuticals.
Downloads
References
Ahmed, I. A. M., Al- Juhaimi, F., Karrar, E., Uslu, N., & Özcan, M. M. (2024). The effect of roasting on bioactive properties, phenolic compounds and fatty acid profiles of pumpkin (Cucurbita spp.) seeds. Journal of Oleo Science, 73(12), 1457-1465. https://doi.org/10.5650/jos.ess24153
Al-Farsi, M., Alasalvar, C., Morris, A., Baron, M., & Shahidi, F. (2005). Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. Journal of Agricultural and Food Chemistry, 53(19), 7592 – 7599. https://doi.org/10.1021/jf050579q
Al-Farsi, M., & Lee, C. Y. (2008). Nutritional and Functional Properties of Dates: A Review. Critical Reviews in Food Science and Nutrition, 48(10), 877-887. https://doi.org/10.1080/10408390701724264
Al-Juhaimi, F. Y., Ghafar, A. A., & Özcan, M. M. (2018). Effect of roasting on physical properties and fatty acid composition of date (Phoenix dactylifera L.) seeds. Food Science and Technology, 38(suppl 1), 146-151.
Ali-Mohamed, A. Y., & Khamis, S. H. (2004). Mineral ion content of the seeds of six cultivars of Bahraini date palm (Phoenix dactylifera). Journal of Agricultural and Food Chemistry, 52(21), 6522-6525. https://doi.org/10.1021/jf030518x
Allaith, A. A. A. (2008). Antioxidant activity of Bahraini date palm (Phoenix dactylifera L.) fruit of various cultivars. International Journal of Food Science & Technology, 43(6), 1033–1040. https://doi.org/10.1111/j.1365-2621.2007.01558.x
AOAC. (2016). Official Methods of Analysis of AOAC International (20th ed.). Association of Official Analytical Chemists. Method 920.39. AOAC International.
Baliga, M. S., Baliga, B. R. V., Kandathil, S. M., Bhat, H. P., & Vayalil, P. K. (2011). A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Research International, 44(7), 1812–1822. https://doi.org/10.1016/j.foodres.2010.07.004
Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239 (1), 70-76. https://doi.org/10.1006/abio.1996.0292
Bhanu, L., Goudar, G., Gogoi, P., Mahajan, A., Subhah, K., Chandragiri, A. K., Sreedhar, M., Dutta, H., Roy, A., Hmar, B. Z., Sharma, P., Sasikumar, R., & Longvah, T. (2024). Effect of roasting on nutritional composition, polyphenols and antioxidant properties of chironji (Buchanania lanzan Spreng) kernels. Journal of Food Measurement and Characterization, 18, 7003-7018. https://doi.org/10.1007/s11694-024-02710-8
Borochov-Neori, H., Judeinstein, S., Greenberg, A., Volkova, N., Rosenblat, M., & Aviram, M. (2015). Antioxidant and antiatherogenic properties of phenolic acid and flavonol fractions of date (Phoenix dactylifera L., Medjool and Hallawi varieties) fruit at different degrees of ripening. Journal of Agricultural and Food Chemistry, 63(12), 3189–3195. https://doi.org/10.1021/jf506489p
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Davey, M. W., Montagu, M. V., Sanmartin, Kanelis, A., Smirnoff, N., Benzie, J. J. JStrain, J. J., Favel, D., & Fletcher, J. (2000). Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. Journal of the Science of Food and Agriculture, 80(7), 825-855.
Elleuch, M., Besbes, S., Roiseux, O., Blecker, C., Deroanne, C., & Attia, H. (2011). Date seeds and kernels: Chemical composition and potential applications. Comprehensive Reviews in Food Science and Food Safety, 10(4), 264-277.
Faleiro, J. R., & Kumar, N. K. K (2023). Date palm- A gift for health and nutrition: National and International scenario. Journal of Horticultural Sciences, 18(2). Food and Agriculture Organization of the United Nations. https://doi.org/10.24154/jhs.v18i2.2508
Fennema, O. R. (1996). Food Chemistry (Vol. 76). CRC press.
Fikry, M., Yusof, Y. A., M. Al-Awaadh, A., Abdul Rahman, R., Chin, N. L., Ghazali, H. M. (2019). Antioxidative and quality properties of full-fat date seeds brew as influenced by the roasting conditions. Antioxidants, 8(7), 226.
Ghafoor, K., Sarker, M. Z. I., Al-Juhaimi, F. Y., Babiker, E. E., & Alkaltham, M. S., Almubarak, A. K. (2022). Extraction and evaluation of bioactive compounds from date (Phoenix dactylifera) seed using supercritical and subcritical CO2 techniques. Foods, 11(12), 1806.
Habib, H. M., Ibrahim, W. H., Jafar, T. T., Rizk, E. M., & Kamal-Eldin, A. (2013). Nutritional quality of date seeds as affected by variety. Emirates Journal of Food and Agriculture, 25(7), 516-522.
Himanshu, K. N., Khangwal, I., & Ashutosh, U. (2024). Assessment of nutritional composition, phytochemical screening, antioxidant and antibacterial activities of date palm (Phoenix dactylifera) seeds. Discover Food, 4, Article 151.https://doi.org/10.1007/s44187-024-00234-0
Morgan, M. T., Ismail, B., & Hayes, K. (2006). Relative importance of the Institute of Food Technologists (IFT) core competencies—A case study survey. Journal of Food Science Education, 5(2), 35-39.
Muñoz-Tebar, N., Candela-Salvador, L., Pérez-Álvarez, J. Á., Lorenzo, J. M., Fernández-López, J., & Viuda-Martos, M. (2024). Date (Phoenix dactylifera L. cv. Medjool) seed flour, a potential ingredient for the food industry: effect of particle size on its chemical, technological, and functional properties. Plants, 13(3), 335.
Ndaw, S., Bergaentzlé, M., Aoudé-Werner, D., & Hasselmann, C. (2000). Extraction procedures for the liquid chromatographic determination of thiamine, riboflavin and vitamin B6 in foodstuffs. Food Chemistry, 71(1), 129–138. https://doi.org/10.1016/S0308-8146(00)00142-7
Nooshkam, M., Varidi, M., & Bashash, M. (2019). The Maillard reaction products as food-born antioxidant and antibrowning agents in model and real food systems. Food Chemistry, 275, 644-660. https://doi.org/10.1016/j.foodchem.2018.09.083
Oboh, G., Ademiluyi, A.O., & Akindahunsi, A. A. (2010). The effect of roasting on the nutritional and antioxidant properties of yellow and white maize varieties. International Journal of Food Science & Technology, 45(6), 1236-1242.https://doi.org/10.1111/j.1365-2621.2010.02263x
Peng, M., Lu, D., Liu, J., Jiang, B., & Chen, J. (2021). Effect of roasting on the antioxidant activity, phenolic composition, and nutritional quality of pumpkin (Cucurbita pepo L.) seeds. Frontiers in Nutrition, 8, 647354.
Rodriguez-Amaya, D. B. (2001). A guide to carotenoid analysis in foods. Washington, DC: International Life Sciences Institute (ILSI) Press. 41 - 47
Rodriguez-Amaya, D. B., & Kimura, M. (2004). HarvestPlus Handbook for Carotenoid Analysis. International Food Policy Research Institute (IFPRI). https://hdl.handle.net/10568/157384
Shahidi, F., & Naczk, M. (2004). Phenolics in food and nutraceuticals. Boca Raton, FL: CRC Press. https://doi.org/10.1201/9780203508732Yousif, G. J., Al-ghamdi, S. S., & Ghnimi, S. (2018). Effect of roasting on the antioxidant activity and total phenolic content of different date seed varieties. Journal of Food Measurement and Characterization, 12(4), 2549-2557.
AOAC. (2016). Official Methods of Analysis of AOAC International (20th ed.). Association of Official Analytical Chemists. Method 923.03. AOAC International.
AOAC. (2016). Official Methods of Analysis of AOAC International (20th ed.). Association of Official Analytical Chemists. Method 925.10. AOAC International.
AOAC. (2016). Official Methods of Analysis of AOAC International (20th ed.). Association of Official Analytical Chemists. Method 962.09. AOAC International.
AOAC. (2016). Official Methods of Analysis of AOAC International (20th ed.). Association of Official Analytical Chemists. Method 984.13. AOAC International.
Baliga, M. S., Baliga, B. R. V., Kandathil, S. M., Bhat, H. P., & Vayalil, P. K. (2011). A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Research International, 44(7), 1812–1822. https://doi.org/10.1016/j.foodres.2010.07.004
Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239 (1), 70-76. https://doi.org/10.1006/abio.1996.0292
Bhanu, L., Goudar, G., Gogoi, P., & Longvah, T. (2024). Effect of roasting on nutritional composition, polyphenols and antioxidant properties of chironji (Buchanania lanzan Spreng) kernels. Journal of Food Measurement and Characterization, 18, 7003-7018. https://doi.org/10.1007/s11694-024-02710-8
Borochov-Neori, H., Judeinstein, S., Greenberg, A., Volkova, N., Rosenblat, M., & Aviram, M. (2015). Antioxidant and antiatherogenic properties of phenolic acid and flavonol fractions of date (Phoenix dactylifera L., Medjool and Hallawi varieties) fruit at different degrees of ripening. Journal of Agricultural and Food Chemistry, 63(12), 3189–3195. https://doi.org/10.1021/jf506489p
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Davey, M. W., Montagu, M. V., Sanmartin, M., Debruyne, L., & Swennen, R. (2000). Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. Journal of the Science of Food and Agriculture, 80(7), 825-855.
Elleuch, M., Besbes, S., Roiseux, O., Blecker, C., Deroanne, C., & Attia, H. (2011). Date seeds and kernels: Chemical composition and potential applications. Comprehensive Reviews in Food Science and Food Safety, 10(4), 264-277.
Faleiro, J. R., & Kumar, N. K. (2024). Date palm- A gift for health and nutrition: National and International scenario. Journal of Horticultural Sciences, 18(2). Food and Agriculture Organization of the United Nations. https://doi.org/10.24154/jhs.v18i2.2508
Fennema, O. R. (Ed.). (1996). Food chemistry (3rd ed.). New York, NY: Marcel Dekker.
Fikry, M., Yusof, Y. A., Al- Awaadh, A. M., Abdul Rahman, R., Chin, N. L., Mousa, E., & Chang, L. S. (2019). Effect of the roasting conditions on the physicochemical, quality and sensory attributes of coffee-like powder and brew from defatted palm date seeds. Foods, 8(2), 61. https://doi.org/10.3390/foods8020061
Ghafoor, K., Sarker, M. Z. I., Al- Juhaimi, F., Y., Babiker, E. E., Alkaltham, M.S., & Almubarak, A. K. (2022). Extraction and evaluation of bioactive compounds from date (Phoenix dactylifera) seed using supercritical and subcritical CO2 techniques. Foods, 11(12), 1806. https://doi.org/10.3390/foods11121806
Habib, H. M., Ibrahim, W. H., Jafar, T. T., Rizk, E. M., & Kamal-Eldin, A. (2013). Nutritional quality of date seeds as affected by variety. Emirates Journal of Food and Agriculture, 25(7), 516-522.
Himanshu, K. N., Khangwal, I., & Ashutosh, U. (2024). Assessment of nutritional composition, phytochemical screening, antioxidant and antibacterial activities of date palm (Phoenix dactylifera) seeds. Discover Food, 4, Article 151. https://doi.org/10.1007/s44187-024-00234-0
Institute of Food Technologists. (2006). Minerals in food: Analysis and bioavailability. Blackwell Publishing.
Mañoz-Tebar, N., Candela-Salvador, L., Pérez-Álvarez, J. Á., Lorenzo, J. M., Fernández-López, J., & Viuda-Martos, M. (2024). Date (Phoenix dactylifera L. cv Medjool) seed flour: Effect of particle size on its chemical, technological and functional properties. Plants, 13(3), 335. https://doi.org/10.3390/plants13030335
Ndaw, S., Bergaentzlé, M., Aoudé-Werner, D., & Hasselmann, C. (2000). Extraction procedures for the liquid chromatographic determination of thiamine, riboflavin and vitamin B6 in foodstuffs. Food Chemistry, 71(1), 129–138. https://doi.org/10.1016/S0308-8146(00)00142-7
Nooshkam, M., Varidi, M., & Bashash, M. (2019). The Maillard reaction products as food-born antioxidant and antibrowning agents in model and real food systems. Food Chemistry, 275, 644-660. https://doi.org/10.1016/j.foodchem.2018.09.083
Oboh, G., Ademiluyi, A.O., & Akindahunsi, A. A. (2010). The effect of roasting on the nutritional and antioxidant properties of yellow and white maize varieties. International Journal of Food Science & Technology, 45(6), 1236-1242. https://doi.org/10.1111/j.1365-2621.2010.02263x
Peng, M., Lu, D., Liu, J., Jiang, B., & Chen. J. (2021). Effect of roasting on the antioxidant activity, phenolic composition and nutritional quality of pumpkin (Cucurbita pepo L.) seeds. Frontiers in Nutrition, 8, Article 647354. https://doi.org/10.3389/fnut.2021.647354
Rodriguez-Amaya, D. B. (2001). A guide to carotenoid analysis in foods. Washington, DC: International Life Sciences Institute (ILSI) Press.
Rodriguez-Amaya, D. B., & Kimura, M. (2004). HarvestPlus Handbook for Carotenoid Analysis. International Food Policy Research Institute (IFPRI).
Shahidi, F., & Naczk, M. (2004). Phenolics in food and nutraceuticals. Boca Raton, FL: CRC Press. https://doi.org/10.1201/9780203508732
Yousif, G. J., Al-ghamdi, S. S., & Ghnimi, S. (2018). Effect of roasting on the antioxidant activity and total phenolic content of different date seed varieties. Journal of Food Measurement and Characterization, 12(4), 2549-2557.
Additional Files
Published
Data Availability Statement
All data generated or analyzed during this study are included in this published article.
Issue
Section
Categories
License
Copyright (c) 2026 Jamila A. Omale, Bintu M. Mofio, Aminu Omale, Funmilola Audu, Palmah M. Nyadar, Suleiman M. Salisu, Sonia O. George, Godwin O. Mohammed, Wisdom O. Oniwon, Samuel A. Egu (Author)

This work is licensed under a Creative Commons Attribution 4.0 International License.